Development of new unstructured model for simultaneous saccharification and fermentation of starch to ethanol by recombinant strain

Abstract The development of simultaneous saccharification and fermentation of starch to ethanol (SSFSE) by genetically modified microbial strains has been studied intensively [M.M. Altintas, B. Kirdar, Z.I. Onsan, K.O. Ulgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445; G. Birol, Z.I. Onsan, B. Kirdar, S.G. Oliver, Ethanol production and fermentation characteristics of recombinant Saccharomyces cerevisiae strains grown on starch, Enzyme Microb. Technol. 22 (1998) 672–677; F. Kobayashi, Y. Nakamura, Effect of repressor gene on stability of bioprocess with continuous conversion of starch into ethanol using recombinant yeast, Biochem. Eng. J. 18 (2004) 133–141; F. Kobayashi, Y. Nakamura, Mathematical model of direct ethanol production from starch in immobilized recombinant yeast culture, Biochem. Eng. J. 21 (2004) 93–101; M.M. Altintas, K.O. Ulgen, B. Kirdar, Z.I. Onsan, S.G. Oliver, Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors, Enzyme Microb. Technol. 31 (2002) 640–647; K.O. Ulgen, B. Saygili, Z.I. Onsan, B. Kirdar, Bioconversion of starch into ethanol by a recombinant Saccharomyces cerevisiae strain YPG-AB, Process Biochem. 37 (2002) 1157–1168]. Saccharomyces cerevisiae YPB-G strain secretes a bifunctional fusion protein containing enzymatic activity of the B. subtilis alpha-amylase and of the Aspergillus awamori glucoamylase [M.M. Altintas, B. Kirdar, Z.I. Onsan, K.O. Ulgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445], and therefore is distinguished in relation to SSFSE step. In this work we have used the experimental data, presented in the paper [M.M. Altintas, B. Kirdar, Z.I. Onsan, K.O. Ulgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445] to develop two-hierarchic-level unstructured mathematical model describing kinetics of direct bioconversion of starch to ethanol. The first level has modeled enzymatic hydrolysis of starch to glucose by bifunctional protein and the second level includes utilization and bioconversion of glucose to ethanol by yeasts. The second level has unified the enzymatic degradation of starch, and glucose metabolization to ethanol by microorganisms. The response surface analysis was used to develop the rates models. A hybrid genetic algorithm and a decomposition approach were used in the nonlinear parameters identification procedure. The proposed model demonstrated excellent flexibility for different operational conditions of SSFSE process, and can be used successfully to describe microbial physiology of genetically modified strains.

[1]  Sing Kiong Nguang,et al.  Modelling and optimization of fed-batch fermentation processes using dynamic neural networks and genetic algorithms , 2004 .

[2]  P. Patnaik Microbial Metabolism as an Evolutionary Response: The Cybernetic Approach to Modeling , 2001, Critical reviews in biotechnology.

[3]  C. A. Reddy,et al.  Fermentation of starch to ethanol by a complementary mixture of an amylolytic yeast andSaccharomycescerevisiae , 2005, Biotechnology Letters.

[4]  S. Oliver,et al.  Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors , 2002 .

[5]  D. Wolf,et al.  Estimating rate constants of heterogeneous catalytic reactions without supposition of rate determining surface steps — an application of a genetic algorithm , 1997 .

[6]  A. Dourado,et al.  Modeling and static optimization of the ethanol production in a cascade reactor. I. Modeling , 1987, Biotechnology and bioengineering.

[7]  Gülnur Birol,et al.  Ethanol production and fermentation characteristics of recombinant saccharomyces cerevisiae strains grown on starch , 1998 .

[8]  Miss A.O. Penney (b) , 1974, The New Yale Book of Quotations.

[9]  R. Amutha,et al.  Production of ethanol from liquefied cassava starch using co-immobilized cells of Zymomonas mobilis and Saccharomyces diastaticus. , 2001, Journal of bioscience and bioengineering.

[10]  Enrico Zio,et al.  Solving the inverse problem of parameter estimation by genetic algorithms: the case of a groundwater contaminant transport model , 2002 .

[11]  H. Kurosawa,et al.  Ethanol production from starch by a coimmobilized mixed culture system of Aspergillus awamori and Zymomonas mobilis , 1986, Biotechnology and bioengineering.

[12]  S. Kilian,et al.  Starch fermentation characteristics of Saccharomyces cerevisiae strains transformed with amylase genes from Lipomyces kononenkoae and Saccharomycopsis fibuligera , 2004 .

[13]  C. Wyman Handbook on bioethanol : production and utilization , 1996 .

[14]  P. Nigam,et al.  Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21 , 2000 .

[15]  C. A. Reddy,et al.  Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae , 1986, Applied and environmental microbiology.

[16]  Venkat Venkatasubramanian,et al.  A hybrid genetic algorithm for efficient parameter estimation of large kinetic models , 2004, Comput. Chem. Eng..

[17]  Subba Rao Somalanka,et al.  Optimization of fermentation conditions for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology. , 2006 .

[18]  A N,et al.  The Dynamics of Microbial Growth on Mixtures of Substrates in Batch Reactors , 1997 .

[19]  M. Polakovič,et al.  Modelling of potato starch saccharification by an Aspergillus niger glucoamylase , 2004 .

[20]  Wang,et al.  A method of graphically analyzing substrate-inhibition kinetics. , 1999, Biotechnology and bioengineering.

[21]  K. Ülgen,et al.  Bioconversion of starch into ethanol by a recombinant Saccharomyces cerevisiae strain YPG-AB , 2002 .

[22]  Transfer function approach in structured modeling of recombinant yeast utilizing starch , 2004 .

[23]  Rudolf Scitovski,et al.  Solving the parameter identification problem of mathematical models using genetic algorithms , 2004, Appl. Math. Comput..

[24]  Betul Kirdar,et al.  Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein , 2002 .

[25]  F. Kobayashi,et al.  Mathematical model of direct ethanol production from starch in immobilized recombinant yeast culture , 2004 .

[26]  F. Kobayashi,et al.  Effect of repressor gene on stability of bioprocess with continuous conversion of starch into ethanol using recombinant yeast , 2003 .

[27]  W. Wiechert,et al.  Experimental design for the identification of macrokinetic models and model discrimination. , 1997, Biotechnology and bioengineering.

[28]  Zsófia Kádár,et al.  Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol , 2004 .

[29]  K. Ulgen,et al.  Flux analysis of recombinant Saccharomyces cerevisiae YPB-G utilizing starch for optimal ethanol production , 2004 .

[30]  P R Patnaik,et al.  Are microbes intelligent beings?: An assessment of cybernetic modeling. , 2000, Biotechnology advances.

[31]  K. Ulgen,et al.  Mathematical description of ethanol fermentation by immobilised Saccharomyces cerevisiae , 1998 .

[32]  G. T. Tsao,et al.  Investigation of bacterial growth on mixed substrates: Experimental evaluation of cybernetic models , 1986, Biotechnology and bioengineering.

[33]  C. Brandama,et al.  An original kinetic model for the enzymatic hydrolysis of starch during mashing , 2022 .

[34]  Colin Webb,et al.  The Biochemical Engineering Journal , 1994 .

[35]  B. Özbek,et al.  σ-Amylase inactivation during corn starch hydrolysis process , 2004 .

[36]  Jiti Zhou,et al.  Simultaneous saccharification and fermentation of potato starch wastewater to lactic acid by Rhizopus oryzae and Rhizopus arrhizus , 2005 .

[37]  K. Schügerl,et al.  Utilization of potato pulp from potato starch processing. , 1993 .

[38]  M. Ueda,et al.  Efficient ethanol production from starch through development of novel flocculent yeast strains displaying glucoamylase and co-displaying or secreting α-amylase , 2002 .

[39]  J. Mielenz,et al.  Ethanol production from biomass: technology and commercialization status. , 2001, Current opinion in microbiology.

[40]  B. Dale,et al.  Global potential bioethanol production from wasted crops and crop residues , 2004 .

[41]  G. T. Tsao,et al.  Cybernetic modeling of microbial growth on multiple substrates , 1984, Biotechnology and bioengineering.

[42]  M. Rodrigues,et al.  Response surface analysis and simulation as a tool for bioprocess design and optimization , 2000 .

[43]  I. Dunn,et al.  Analysis of the performance of a two-stage fermentor with cell recycle for continuous ethanol production using different kinetic models , 1999 .

[44]  T. Peeples,et al.  Kinetic enhancement of starch bioconversion in thermoseparating aqueous two-phase reactor systems , 2002 .

[45]  Brian H. Davison,et al.  Economic analysis of fuel ethanol production from corn starch using fluidized-bed bioreactors , 2000 .

[46]  Manish K. Singh,et al.  Genetic algorithm to estimate interaction parameters of multicomponent systems for liquid-liquid equilibria , 2005, Comput. Chem. Eng..