Histochemical studies on the Leydig-cell-leucine aminopeptidase activity in the guinea-pig testis.

The activity pattern and functional significance of leucine aminopeptidase (LAP) have been studied in the testes of oestrogen\x=req-\ treated, cryptorchid and HCG-treated guinea-pigs. The enzyme was present only in the Leydig cells and showed marked fluctuations in its activity pattern, which correlated closely with alterations in the functional activities of the Leydig cells. Hyperfunctioning Leydig cells exhibited enhanced LAP activity, whereas hypofunctioning of these intertubular cells resulted in a diminution in the enzyme activity. The LAP activity in the intertubular Leydig cells may be regarded as a cytochemical marker enzyme in the assessment of Leydig cell function in the guinea-pig testes under varying experimental conditions.

[1]  S. Dey,et al.  Testicular leucine aminopeptidase in different vertebrates. , 1973, Acta histochemica.

[2]  V. Gay,et al.  Effects of testosterone propionate and estradiol benzoate--alone or in combination--on serum LH and FSH in orchidectomized rats. , 1971, Endocrinology.

[3]  A. Kaneyoshi.  [Effect of gonadotropin on aminopeptidase activities in the rat ovary]. , 1969, Nihon Naibunpi Gakkai zasshi.

[4]  H. Inano,et al.  Effect of experimental bilateral cryptorchidism on testicular enzymes related to androgen formation. , 1968, Endocrinology.

[5]  L. Lerner,et al.  The effect of antagonists of androgen- and estrogen-induced changes in the male accessory sex organs of the rat. , 1968, Transactions of the New York Academy of Sciences.

[6]  J. Morehead,et al.  Cryptorchidism. Its pre- and postpubertal effects on the hypophysis of the rat. , 1967, Fertility and sterility.

[7]  H. Oshima,et al.  The effect of synthetic estrogen upon the biosynthesis in vitro of androgeng and luteinizing hormone in the rat. , 1967, Biochimica et biophysica acta.

[8]  J. Skinner,et al.  Effect of unilateral cryptorchidism on sexual development in the pubescent male animal. , 1967, Journal of reproduction and fertility.

[9]  L. Ewing,et al.  On the formation of testosterone by the perfused rabbit testis. , 1966, Canadian journal of biochemistry.

[10]  K. Eik‐Nes.  Secretion of testosterone by the eutopic and the cryptorchid testes in the same dog. , 1966, Canadian journal of physiology and pharmacology.

[11]  E. Tonutti,et al.  [The reaction of some dehydrogenases in the testis of the rat, guinea-pig and mouse after estrogen treatment]. , 1966, Acta histochemica.

[12]  M. Kormano,et al.  EFFECT OF EXPERIMENTAL CRYPTORCHIDISM ON THE HISTOCHEMICALLY DEMONSTRABLE DEHYDROGENASES OF THE RAT TESTIS. , 1964, Endocrinology.

[13]  J. G. Llaurado,et al.  Effect of cryptorchidism on testicular enzymes involved in androgen biosynthesis. , 1963, Endocrinology.

[14]  S. Talanti,et al.  Leucine Aminopeptidase in the Mammary Gland of the Cow and Rat , 1961, Nature.

[15]  H. W. Deane,et al.  Enzymic correlates of physiological regression of follicles and corpora lutea in ovaries of normal rats. , 1961, Endocrinology.

[16]  S. Talanti,et al.  Leucine aminopeptidase in the gastric muscosa of the adrenalectomized rat. , 1961, Endocrinology.

[17]  S. Talanti,et al.  The influence of methyl-thiouracil on the leucine aminopeptidase activity in the thyroid gland of the rat. A histochemical study. , 1960, Acta endocrinologica.

[18]  A. Seligman,et al.  Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, L-Leucyl-4-Methoxy-2-Naphthylamide , 1960, The Journal of biophysical and biochemical cytology.

[19]  A. Pearse,et al.  Leucine Aminopeptidase in Rat Parathyroid and its Relation to Parathyroid Hormone Production , 1958, Nature.

[20]  T. Brinck-Johnsen,et al.  Effect of human chorionic gonadotropin on the secretion of testosterone and 4-androstene-3,17-dione by the canine testis. , 1957, Endocrinology.

[21]  W. C. Young,et al.  Internal secretory capacity of the abdominal testis in the guinea pig. , 1957, Endocrinology.

[22]  L. Samuels,et al.  The influence of chorionic gonadotropin on the 3beta-ol dehydrogenase activity of testes and adrenals. , 1956, Endocrinology.

[23]  K. Lynch.  RECOVERY OF THE RAT TESTIS FOLLOWING ESTROGEN THERAPY , 1952, Annals of the New York Academy of Sciences.

[24]  W. Scott,et al.  Lipid distribution in the Sertoli cell and Leydig cell of the rat testis as related to experimental alterations of the pituitary-gonad system. , 1951, Endocrinology.

[25]  C. R. Moore.  Hormone Secretion by Experimental Cryptorchid Testes * , 1944, The Yale journal of biology and medicine.