论文信息 - Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas as an alternative to Soxhlet and ultrasound-assisted extraction.

Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas as an alternative to Soxhlet and ultrasound-assisted extraction.

Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 degrees C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.

[1] J. Clayton,et al. INVESTIGATIONS RELATIVE TO THE BREEDING OF COUMARIN-FREE SWEET CLOVER, MELILOTUS , 1936 .

[2] M. Pârvu,et al. Effects of Melilotus officinalis on acute inflammation , 2002, Phytotherapy research : PTR.

[3] F. Lanças,et al. Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves , 2001 .

[4] T. Dzido,et al. Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems. , 2000, Journal of chromatography. A.

[5] A. Guckert,et al. Extraction of coumarins from plant material (Leguminosae) , 1994 .

[6] M. Pfister,et al. HPLC analysis of tonka bean extracts , 1995, Zeitschrift fur Lebensmittel-Untersuchung und -Forschung.

[7] C. Levy,et al. The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase. , 1966, The Journal of biological chemistry.

[8] G. Innocenti,et al. Furocoumarin contents in the vegetative organs of cultivated Psoralea species , 1991 .

[9] P. Christen,et al. Focused microwave-assisted extraction of cocaine and benzoylecgonine from coca leaves. , 2002, Phytochemical analysis : PCA.

[10] D. Wianowska,et al. Effect of extraction method on the yield of furanocoumarins from fruits of Archangelica officinalis Hoffm. , 2004, Phytochemical analysis : PCA.