Use of Chlorella vulgaris and Ulva lactuca as Biostimulant on Lettuce

The important purpose of this work is to evaluate the biostimulant activity of the algae Chlorella vulgaris and Ulva lactuca extracts on the crop plants Lactuca sativa, to compare the effect of these two green algae on plant growth and development as a part of a sustainable plant production method and show that these extracts can be a promissory source for replacing chemical fertilization. The study faces all the phases of plant growth, from the germination of the seeds to the greenhouse plant growth and treatment, matched with the chemical characterization of both the green algae used and the lettuce plants. This work is meant to define a tool to be improved by more experiments and studies in order to suggest a sustainable method that could ensure an adequate use of organic fertilizer. After the identification of the best concentration of the respective extracts of C. vulgaris and U. lactuca, the experiment affirmed that a low concentration (15% C. vulgaris and 25% U. lactuca) of the extracts contributed to the production of plants with a satisfying nutritional profile, while a high concentration (75% U. lactuca) is conducive to lettuce production with some parameters not suitable for human diet and health.

[1]  R. Kapoore,et al.  Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices. , 2021, Biotechnology advances.

[2]  David Rodrigues,et al.  Agenda 2030 , 2020, Quaestio - Revista de Estudos em Educação.

[3]  A. Mathys,et al.  Biochemical and Nutritional Evaluation of Chlorella and Auxenochlorella Biomasses Relevant for Food Application , 2020, Frontiers in Nutrition.

[4]  Y. Rouphael,et al.  Metabolomic Responses of Maize Shoots and Roots Elicited by Combinatorial Seed Treatments With Microbial and Non-microbial Biostimulants , 2020, Frontiers in Microbiology.

[5]  A. Razin,et al.  Analysis of the world lettuce market , 2019, IOP Conference Series: Earth and Environmental Science.

[6]  P. Cunniff Official Methods of Analysis of AOAC International , 2019 .

[7]  H. Domínguez,et al.  Ulva lactuca, A Source of Troubles and Potential Riches , 2019, Marine drugs.

[8]  C. M. Geraldson,et al.  Plant Analysis as an Aid in Fertilizing Vegetable Crops , 2018, SSSA Book Series.

[9]  Theodore Karyotis,et al.  Zinc in soils, water and food crops. , 2018, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[10]  M. Ruzzi,et al.  Biostimulant Action of Protein Hydrolysates: Unraveling Their Effects on Plant Physiology and Microbiome , 2017, Front. Plant Sci..

[11]  A. Rasyid Evaluation of Nutritional Composition of The Dried Seaweed Ulva lactuca from Pameungpeuk Waters, Indonesia , 2017, Tropical life sciences research.

[12]  O. I. Yakhin,et al.  Biostimulants in Plant Science: A Global Perspective , 2017, Frontiers in plant science.

[13]  Moo Jung Kim,et al.  Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.) , 2016 .

[14]  R. Nys,et al.  The protein content of seaweeds: a universal nitrogen-to-protein conversion factor of five , 2016, Journal of Applied Phycology.

[15]  Carlos Vaca-Garcia,et al.  Morphology, composition, production, processing and applications of Chlorella vulgaris: A review , 2014 .

[16]  M. Ruiz-López,et al.  Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.) , 2014, Journal of Applied Phycology.

[17]  H. Khairy,et al.  Seasonal variations in the biochemical composition of some common seaweed species from the coast of Abu Qir Bay, Alexandria, Egypt , 2013 .

[18]  H. Attia,et al.  Chemical composition and functional properties of Ulva lactuca seaweed collected in Tunisia , 2011, Food Chemistry.

[19]  A. Farid,et al.  Rapid bioassays to evaluate the plant growth promoting activity of Ascophyllum nodosum (L.) Le Jol. using a model plant, Arabidopsis thaliana (L.) Heynh , 2008, Journal of Applied Phycology.

[20]  O. Tokuşoglu,et al.  Biomass Nutrient Profiles of Three Microalgae: Spirulina platensis, Chlorella vulgaris, and Isochrisis galbana , 2003 .

[21]  S. Djuric,et al.  Influence of green algae Chlorella vulgaris on initial growth of different agricultural crops , 2016 .

[22]  S. Savcı Investigation of Effect of Chemical Fertilizers on Environment , 2012 .

[23]  S. Hassan,et al.  Bioactivity of Ulva lactuca L. acetone extract on germination and growth of lettuce and tomato plants , 2009 .