1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay.

A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.

[1]  H. DeLuca,et al.  High-pressure liquid chromatography: separation of the metabolites of vitamins D2 and D3 on small-particle silica columns. , 1975, Journal of lipid research.

[2]  H. DeLuca,et al.  The mobilization of bone mineral by 1,25-dihydroxyvitamin D3 in hypophosphatemic rats. , 1975, Endocrinology.

[3]  M. Haussler,et al.  Specific binding of 1alpha,25-dihydroxycholecalciferol to nuclear components of chick intestine. , 1975, The Journal of biological chemistry.

[4]  M. Haussler,et al.  Filter assay for 1alpha, 25-dihydroxyvitamin D3. Utilization of the hormone's target tissue chromatin receptor. , 1974, Biochemistry.

[5]  H. DeLuca,et al.  Role of vitamin D metabolites in phosphate transport of rat intestine. , 1974, The Journal of nutrition.

[6]  H. DeLuca,et al.  Role of 1,25-dihydroxyvitamin D3 in maintaining serum phosphorus and curing rickets. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[7]  H. DeLuca,et al.  Intestinal Calcium Transport: Stimulation by Low Phosphorus Diets , 1973, Science.

[8]  H. DeLuca,et al.  The control of 25-hydroxyvitamin D metabolism by inorganic phosphorus. , 1973, Archives of biochemistry and biophysics.

[9]  H. DeLuca,et al.  Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[10]  H. DeLuca,et al.  The response of intestinal calcium transport to 25-hydroxy and 1,25-dihydroxy vitamin D in nephrectomized rats. , 1972, Endocrinology.

[11]  H. DeLuca,et al.  Bone mineral mobilization activity of 1,25-dihydroxycholecalciferol, a metabolite of vitamin D. , 1971, Archives of biochemistry and biophysics.

[12]  H. DeLuca,et al.  Regulation by calcium of in vivo synthesis of 1,25-dihydroxycholecalciferol and 21,25-dihydroxycholecalciferol. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[13]  H. DeLuca,et al.  Synthesis of (26,27- 3 H)-25-hydroxycholecalciferol. , 1971, Analytical biochemistry.

[14]  H. DeLuca,et al.  Biological activity of 1,25-dihydroxycholecalciferol. , 1971, Biochemistry.

[15]  H. DeLuca,et al.  Vitamin D Metabolism: The Role of Kidney Tissue , 1971, Science.

[16]  A. Norman,et al.  Vitamin D: A Cholecalciferol Metabolite Highly Active in Promoting Intestinal Calcium Transport , 1971, Science.

[17]  D. Fraser,et al.  Unique Biosynthesis by Kidney of a Biologically Active Vitamin D Metabolite , 1970, Nature.

[18]  H. DeLuca,et al.  Metabolites of vitamin D3 and their biologic activity. , 1969, The Journal of nutrition.

[19]  M. Haussler,et al.  A rapidly acting metabolite of vitamin D3. , 1971, Proceedings of the National Academy of Sciences of the United States of America.