Etude de structures peptidiques à l'aide du phénylisothiocyanate-[35S]

The use of [35S]-phenylisothiocyanate (EDMAN's reagent, with radioactif tracer) for the determination of the sequence of amino acids in peptides is described. With an activity of 15 mc/mM in this reagent, quantities of 0.005 to 0.01 micromole of peptide (di- and tri-peptides) can be used. With certain amino acids, parasite pics of still unknown nature appear.