Molecular cloning of canine RCAS1 cDNA.

Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen, strongly expressed in invasive cancers. RCAS1 inhibited the in vitro growth of immunocytes, and induced apoptotic cell death. The cloning of canine RCAS1 cDNA was carried out and identified from the mammary gland tumor of a dog. A canine RCAS1 cDNA of 864 bp in length has an open reading frame of 642 bp nucleotides encoding a protein of 213 deduced amino acids. The predicted amino acid sequence of canine RCAS1 showed 96.2% and 96.7% homologies with those of human and mouse RCAS1 respectively. Canine RCAS1 has an N-terminal transmembrane segment and a coiled-coil structure in the C-terminal protein, which are highly conserved in mouse and human RCAS1.

[1]  T. Oshikiri,et al.  The prognostic significance of RCAS1 expression in squamous cell carcinoma of the oesophagus. , 2002, Cancer letters.

[2]  R. Osamura,et al.  Immunohistochemical Analysis of RCAS1 in Human Pituitary Adenomas , 2001, Modern Pathology.

[3]  S. Inoue,et al.  EBAG9/RCAS1 in human breast carcinoma: a possible factor in endocrine–immune interactions , 2001, British Journal of Cancer.

[4]  H. Nawata,et al.  Aberrant intracellular localization of RCAS1 is associated with tumor progression of gastric cancer. , 2001, International journal of oncology.

[5]  K. Sugimachi,et al.  Expression of a tumor-associated antigen RCAS1 in hepatocellular carcinoma. , 2001, Cancer letters.

[6]  Y. Nakanishi,et al.  Expression of tumor‐associated antigen RCAS1 correlates significantly with poor prognosis in nonsmall cell lung carcinoma , 2001, Cancer.

[7]  K. Ohshima,et al.  Expression of RCAS1 and FasL in human trophoblasts and uterine glands during pregnancy: the possible role in immune privilege , 2001, Clinical and experimental immunology.

[8]  T. Kaku,et al.  The clinical significance of tumor-associated antigen RCAS1 expression in the normal, hyperplastic, and malignant uterine endometrium. , 2000, Gynecologic oncology.

[9]  M. Nakashima,et al.  Expression and prognostic significance in lung cancer of human tumor‐associated antigen RCAS1 , 2000, International journal of cancer.

[10]  M. Nakashima,et al.  Inhibition of cell growth and induction of apoptotic cell death by the human tumor-associated antigen RCAS1 , 1999, Nature Medicine.

[11]  T. Kaku,et al.  The prognostic significance of tumor-associated antigen 22-1-1 expression in adenocarcinoma of the uterine cervix. , 1999, Clinical cancer research : an official journal of the American Association for Cancer Research.

[12]  K. Tham,et al.  A preliminary study of the immunohistochemical detection of a novel tumour marker, 22-1-1 antigen, in gynaecological cancer specimens. , 1999, Annals of the Academy of Medicine, Singapore.

[13]  T. Kaku,et al.  Tumor-associated antigen 22-1-1 expression in the uterine cervical squamous neoplasias. , 1998, Clinical cancer research : an official journal of the American Association for Cancer Research.

[14]  S. Inoue,et al.  Isolation of Estrogen-Responsive Genes with a CpG Island Library , 1998, Molecular and Cellular Biology.

[15]  T. Kaku,et al.  A novel tumor‐associated antigen expressed in human uterine and ovarian carcinomas , 1996, Cancer.

[16]  Kay Hofmann,et al.  Tmbase-A database of membrane spanning protein segments , 1993 .