Mechanical force response of single living cells using a microrobotic system

In this paper, we investigate mechanical force response of single living cells at different conditions using a microrobotic system. Zebrafish eggs at different developmental stages were collected and an integrated biomanipulation system was employed to measure cellular force during penetrating the egg envelope, the chorion. First, the biomanipulation system integrated with cellular force sensing instrument is implemented to measure the penetration force of the chorion envelope and then to characterize mechanical properties of zebrafish embryos. Second, the cellular force sensing of penetrating the chorion envelope at each developmental stages was experimentally performed. The results demonstrated that the biomanipulation system with force sensing capability can measure cellular force in real-time while the injection operation is undergoing. The magnitude of the measured cellular force decrease as an embryo develops. This result quantitatively describes the chorion softening in zebrafish embryos. Experimental results also demonstrate that subtle modification of the chorion, the extracellular matrix of the egg, can be monitored physically using the developed real-time force sensing system.

[1]  Kok-Kiong Tan,et al.  Optimal intra-cytoplasmic sperm injection with a piezo micromanipulator , 2002, Proceedings of the 4th World Congress on Intelligent Control and Automation (Cat. No.02EX527).

[2]  H. Fujiwara,et al.  A new assisted hatching technique using a piezo-micromanipulator. , 1998, Fertility and sterility.

[3]  B. H. Campbell,et al.  A multi-station culture force monitor system to study cellular contractility. , 2003, Journal of biomechanics.

[4]  K.K. Tan,et al.  Computer controlled piezo micromanipulation system for biomedical applications , 2001 .

[5]  Didier Y. R. Stainier,et al.  Zebrafish genetics and vertebrate heart formation , 2001, Nature Reviews Genetics.

[6]  R Yanagimachi,et al.  Intracytoplasmic sperm injection in the mouse. , 1995, Biology of reproduction.

[7]  N. Xi,et al.  A high sensitivity force sensor for microassembly: design and experiments , 2003, Proceedings 2003 IEEE/ASME International Conference on Advanced Intelligent Mechatronics (AIM 2003).

[8]  N. Caille,et al.  Contribution of the nucleus to the mechanical properties of endothelial cells. , 2002, Journal of biomechanics.

[9]  F Sachs,et al.  The breakdown of cell membranes by electrical and mechanical stress. , 1998, Biophysical journal.

[10]  Kok Kiong Tan,et al.  Optimal Intra-Cytoplasmic Sperm Injection with a Piezo Micromanipulator , 2004, Control. Intell. Syst..

[11]  M Radmacher,et al.  Measuring the elastic properties of biological samples with the AFM. , 1997, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society.

[12]  김병규,et al.  Cellular force measurement for force feedback-based biomanipulation , 2003 .

[13]  Bradley J. Nelson,et al.  Biological Cell Injection Using an Autonomous MicroRobotic System , 2002, Int. J. Robotics Res..

[14]  H. Katayose,et al.  The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. , 1999, Human reproduction.

[15]  Lewis Wolpert,et al.  Principles of Development , 1997 .

[16]  Imad H. Elhajj,et al.  A 2-D PVDF force sensing system for micro-manipulation and micro-assembly , 2002, Proceedings 2002 IEEE International Conference on Robotics and Automation (Cat. No.02CH37292).