Quantitative visualization of pectin distribution maps of peach fruits

Pectin content is an important quality index of fruits, as pectin content undergoes significant changes during the peach ripening process. The commonly used carbazole colorimetry method measures only the total content value of each kind of pectin for each pulp sample and cannot provide distribution maps of the pectin contents for the whole fruit pulp. This work used the hyperspectral imaging technique to quantitatively visualize the distribution maps of pectin contents inside peach pulp at the pixel level. The protopectin contents were well predicted, with the best residual predictive deviation of 2.264, whereas the predictions of the water-soluble pectin and the total pectin contents were not satisfied. On the basis of the best predictive model, the distribution maps of the protopectin contents were quantitatively visualized. A histogram of an example protopectin distribution revealed the existence of a wide range of protopectin contents in peach pulp. Our results show that hyperspectral imaging holds promise as a powerful alternative to the carbazole colorimetry method for measuring the spatial variations in the protopectin distribution inside peach pulp. The distribution maps could be used as a maturity indicator to understand and evaluate the ripening process of peach fruit in depth.

[1]  Yong He,et al.  Comparison of Infrared Spectroscopy and Nuclear Magnetic Resonance Techniques in Tandem with Multivariable Selection for Rapid Determination of ω-3 Polyunsaturated Fatty Acids in Fish Oil , 2014, Food and Bioprocess Technology.

[2]  Yidan Bao,et al.  Rapid prediction of moisture content of dehydrated prawns using online hyperspectral imaging system. , 2012, Analytica chimica acta.

[3]  R. Visser,et al.  If Homogalacturonan Were a Side Chain of Rhamnogalacturonan I. Implications for Cell Wall Architecture1 , 2003, Plant Physiology.

[4]  I. Brownlee The physiological roles of dietary fibre , 2011 .

[5]  B. T. Stokke,et al.  The relation of apple texture with cell wall nanostructure studied using an atomic force microscope. , 2013, Carbohydrate polymers.

[6]  Yuan-Chuen Wang,et al.  The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan , 2008 .

[7]  Jizhan Liu,et al.  Fruit biomechanics based on anatomy: a review , 2013 .

[8]  Di Wu,et al.  Advanced applications of hyperspectral imaging technology for food quality and safety analysis and assessment: A review — Part II: Applications , 2013 .

[9]  Hongshun Yang,et al.  Changes in firmness, pectin content and nanostructure of two crisp peach cultivars after storage , 2010 .

[10]  H. Jia,et al.  Effect of fertilizer application level on pectin composition of Hakuho peach (Prunus persica Batsch) during maturation , 2006 .

[11]  R. Lu,et al.  Analysis of spatially resolved hyperspectral scattering images for assessing apple fruit firmness and soluble solids content , 2008 .

[12]  E. Mehinagic,et al.  Relationship between texture and pectin composition of two apple cultivars during storage , 2008 .

[13]  G. Phillips,et al.  An introduction: Evolution and finalisation of the regulatory definition of dietary fibre , 2011 .

[14]  Gamal ElMasry,et al.  Non-destructive determination of water-holding capacity in fresh beef by using NIR hyperspectral imaging , 2011 .

[15]  Umezuruike Linus Opara,et al.  Estimating blueberry mechanical properties based on random frog selected hyperspectral data , 2015 .

[16]  Di Wu,et al.  Study on infrared spectroscopy technique for fast measurement of protein content in milk powder based on LS-SVM , 2008 .

[17]  Di Wu,et al.  Potential of time series-hyperspectral imaging (TS-HSI) for non-invasive determination of microbial spoilage of salmon flesh. , 2013, Talanta.

[18]  N. Blumenkrantz,et al.  New method for quantitative determination of uronic acids. , 1973, Analytical biochemistry.

[19]  Pengcheng Nie,et al.  Application of Time Series Hyperspectral Imaging (TS-HSI) for Determining Water Distribution Within Beef and Spectral Kinetic Analysis During Dehydration , 2013, Food and Bioprocess Technology.

[20]  E. Mellerowicz,et al.  New insights into pectin methylesterase structure and function. , 2007, Trends in plant science.

[21]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[22]  Shao-qian Cao,et al.  Pectin plays an important role on the kinetics properties of polyphenol oxidase from honeydew peach. , 2015, Food chemistry.

[23]  A. Malkin,et al.  Imaging Cell Wall Architecture in Single Zinnia elegans Tracheary Elements1[OA] , 2010, Plant Physiology.

[24]  Yong He,et al.  Potential of hyperspectral imaging and multivariate analysis for rapid and non-invasive detection of gelatin adulteration in prawn , 2013 .

[25]  Renfu Lu,et al.  Assessing Multiple Quality Attributes of Peaches Using Optical Absorption and Scattering Properties , 2012 .

[26]  Bernhard Spengler,et al.  High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas. , 2015, The Analyst.

[27]  Hongshun Yang,et al.  Atomic force microscopy of the water-soluble pectin of peaches during storage , 2005 .

[28]  A. Zdunek,et al.  The self-assembled network and physiological degradation of pectins in carrot cell walls , 2015 .

[29]  K. Tu,et al.  Detection of cold injury in peaches by hyperspectral reflectance imaging and artificial neural network. , 2016, Food chemistry.

[30]  J. Labavitch,et al.  Cell wall metabolism during maturation, ripening and senescence of peach fruit. , 2004, Journal of experimental botany.

[31]  A. Zdunek,et al.  Combining FT-IR spectroscopy and multivariate analysis for qualitative and quantitative analysis of the cell wall composition changes during apples development. , 2015, Carbohydrate polymers.

[32]  Elspeth MacRae,et al.  In vivo and in vitro swelling of cell walls during fruit ripening , 1997, Planta.

[33]  Di Wu,et al.  Potential of spectroscopic techniques and chemometric analysis for rapid measurement of docosahexaenoic acid and eicosapentaenoic acid in algal oil. , 2014, Food chemistry.

[34]  Ning Wang,et al.  Studies on banana fruit quality and maturity stages using hyperspectral imaging , 2012 .

[35]  Haijun Zhang,et al.  Melatonin promotes ripening and improves quality of tomato fruit during postharvest life , 2014, Journal of experimental botany.

[36]  Fan Zhao,et al.  Nondestructive Measurement of Soluble Solids Content of Kiwifruits Using Near-Infrared Hyperspectral Imaging , 2015, Food Analytical Methods.

[37]  J. Mikkelsen,et al.  Pectin: new insights into an old polymer are starting to gel , 2006 .