Carrots of many colors provide basic nutrition and bioavailable phytochemicals acting as a functional food.

ABSTRACT:  Hippocrates, a philosopher who lived from 460 to 359 BC is often quoted as saying, “Let your food be thy medicine and your medicine be thy food.” Having lived just shy of a century at a time when life expectancies were much less, he must have understood the importance of a healthy diet. A diet high in fruit and vegetables has been linked to optimal health in a variety of studies. One vegetable that has gained popularity is the carrot due in part to the introduction of “cut & peel” convenience packages. Although most people in the United States know carrots as an orange vegetable that can be eaten raw or in a variety of cooked dishes, original carrots were yellow and purple. These carrot varieties are currently undergoing phenotypic recurrent selection to improve the profile of compounds considered to be beneficial. This process is called biofortification, which has increased provitamin A content by >40% since 1970. The most novel carrot produced to date is an orange–purple–red variety that not only contains provitamin A activity as α- and β-carotene, but also contains anthocyanins and the nonprovitamin A carotenoid lycopene, of which both are potent antioxidants. A functional food is one that provides benefit beyond basic nutrition. Biofortified carrots of many colors not only provide vitamin A, but may contribute to optimal health. Because supplements have not been shown to be overly beneficial, except for correcting deficiencies, whole food-based approaches to enhance health by utilizing functional foods such as biofortified carrots should be considered.

[1]  S. W. Souci,et al.  Food composition and nutrition tables. , 2000 .

[2]  A. Ghiselli,et al.  Antioxidant Activity of Different Phenolic Fractions Separated from an Italian Red Wine. , 1998, Journal of agricultural and food chemistry.

[3]  R. C. Lindsay,et al.  Correlations between sensory and objective parameters of carrot flavor. , 1980 .

[4]  Bernhard Watzl,et al.  Supplementation of a Low-Carotenoid Diet with Tomato or Carrot Juice Modulates Immune Functions in Healthy Men , 2003, Annals of Nutrition and Metabolism.

[5]  Y. Shoenfeld,et al.  NEUTROPENIA INDUCED BY HYPERCAROTENAEMIA , 1982, The Lancet.

[6]  K. Scott,et al.  Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK , 1995 .

[7]  S. Tanumihardjo Assessing vitamin A status: past, present and future. , 2004, The Journal of nutrition.

[8]  F. Q. Schafer,et al.  Comparing beta-carotene, vitamin E and nitric oxide as membrane antioxidants. , 2002, Biological chemistry.

[9]  J. Hautvast,et al.  Orange fruit is more effective than are dark-green, leafy vegetables in increasing serum concentrations of retinol and beta-carotene in schoolchildren in Indonesia. , 1998, The American journal of clinical nutrition.

[10]  D. Barnes,et al.  Purple carrot (Daucus carota L.) polyacetylenes decrease lipopolysaccharide-induced expression of inflammatory proteins in macrophage and endothelial cells. , 2008, Journal of agricultural and food chemistry.

[11]  P. Jing,et al.  Structure-function relationships of anthocyanins from various anthocyanin-rich extracts on the inhibition of colon cancer cell growth. , 2008, Journal of agricultural and food chemistry.

[12]  L. Pike,et al.  A method for measuring anthocyanins after removing carotenes in purple colored carrots , 2001 .

[13]  P. Ramulu,et al.  Total, insoluble and soluble dietary fiber contents of Indian fruits , 2003 .

[14]  Xianzhong Wu,et al.  Antioxidant and antiproliferative activities of common vegetables. , 2002, Journal of agricultural and food chemistry.

[15]  S. Lafay,et al.  Chlorogenic acids from green coffee extract are highly bioavailable in humans. , 2008, The Journal of nutrition.

[16]  Lindsay H Allen,et al.  Estimating the potential for vitamin A toxicity in women and young children. , 2002, The Journal of nutrition.

[17]  Y. L. Tang,et al.  The bioavailability of beta-carotene in stir- or deep-fried vegetables in men determined by measuring the serum response to a single ingestion. , 2000, The Journal of nutrition.

[18]  B. Underwood Vitamin A deficiency disorders: international efforts to control a preventable "pox". , 2004, The Journal of nutrition.

[19]  D. Baer,et al.  Bioavailability of anthocyanins from purple carrot juice: effects of acylation and plant matrix. , 2009, Journal of agricultural and food chemistry.

[20]  S. Tanumihardjo Factors influencing the conversion of carotenoids to retinol: bioavailability to bioconversion to bioefficacy. , 2002, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[21]  P. Lea,et al.  The effect of temperature on sensory quality, chemical composition and growth of carrots (Daucus carota L.) I. Constant diurnal temperature , 1998 .

[22]  J. Herman,et al.  Carotenoids and the risk of developing lung cancer: a systematic review. , 2008, The American journal of clinical nutrition.

[23]  G. Rechkemmer,et al.  Consumption of vegetables reduces genetic damage in humans: first results of a human intervention trial with carotenoid-rich foods. , 1997, Carcinogenesis.

[24]  Delia B. Rodriguez-Amaya,et al.  Updated Brazilian database on food carotenoids: Factors affecting carotenoid composition , 2008 .

[25]  J. Erdman,et al.  Absorption and Transport of Carotenoids , 1993, Annals of the New York Academy of Sciences.

[26]  Agnieszka Nawirska,et al.  Dietary fibre fractions from fruit and vegetable processing waste , 2005 .

[27]  G. Lucier,et al.  Factors affecting carrot consumption in the United States. , 2011 .

[28]  P. Di Mascio,et al.  Lycopene as the most efficient biological carotenoid singlet oxygen quencher. , 1989, Archives of biochemistry and biophysics.

[29]  G. Frost,et al.  Increased sucrose intake is not associated with a change in glucose or insulin sensitivity in people with type 2 diabetes , 2007, International journal of food sciences and nutrition.

[30]  T. Hofmann,et al.  Quantitative studies and sensory analyses on the influence of cultivar, spatial tissue distribution, and industrial processing on the bitter off-taste of carrots (Daucus carota l.) and carrot products. , 2004, Journal of agricultural and food chemistry.

[31]  Jafar M. El-Qudah,et al.  Identification and Quantification of Major Carotenoids in Some Vegetables , 2009 .

[32]  Fereidoon Shahidi,et al.  Effect of chill storage and modified atmosphere packaging (MAP) on antioxidant activity, anthocyanins, carotenoids, phenolics and sensory quality of ready-to-eat shredded orange and purple carrots , 2005 .

[33]  R. Baranski,et al.  Tissue-specific accumulation of carotenoids in carrot roots , 2006, Planta.

[34]  J. Rivas,et al.  ISOLATION OF A YELLOW CAROTENOPROTEIN FROM CARROT , 1991 .

[35]  F. Granado,et al.  Seasonal and sex-related variations in six serum carotenoids, retinol, and alpha-tocopherol. , 1994, The American journal of clinical nutrition.

[36]  R. Welch,et al.  The effects of the fibre content and physical structure of carrots on satiety and subsequent intakes when eaten as part of a mixed meal. , 2006, The British journal of nutrition.

[37]  P. Baardseth,et al.  Evaluation of carrot varieties for production of deep fried carrot chips—IV. The influence of growing environment on carrot raw material , 1997 .

[38]  J. Reed Cranberry Flavonoids, Atherosclerosis and Cardiovascular Health , 2002, Critical reviews in food science and nutrition.

[39]  P. Lea,et al.  The effect of temperature on sensory quality, chemical composition and growth of carrots (Daucus carota L.). III. Different diurnal temperature amplitudes , 1999 .

[40]  J. Robertson,et al.  The effect of raw carrot on serum lipids and colon function. , 1979, The American journal of clinical nutrition.

[41]  J. Loscalzo,et al.  Dietary antioxidants preserve endothelium-dependent vessel relaxation in cholesterol-fed rabbits. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[42]  M. Gajewski,et al.  Some Aspects of Nutritive and Biological Value of Carrot Cultivars with Orange, Yellow and Purple-Coloured Roots , 2007 .

[43]  T. McGhie,et al.  The bioavailability and absorption of anthocyanins: towards a better understanding. , 2007, Molecular nutrition & food research.

[44]  J. Keaney,et al.  Antioxidants and atherosclerotic heart disease. , 1997, The New England journal of medicine.

[45]  G. Mazza Anthocyanins and heart health. , 2007, Annali dell'Istituto superiore di sanita.

[46]  Agnieszak Nawirska,et al.  Waste products from fruit and vegetable processing as potential sources for food enrichment in dietary fibre , 2008 .

[47]  S. K. Sarkar,et al.  PHYSICAL AND CHEMICAL CHANGES OCCURRING IN THE CARROT ROOT DURING STORAGE , 1973 .

[48]  G. Fahey,et al.  The accumulation of α- and β-carotene in serum and tissues of preruminant calves fed raw and steamed carrot slurries , 1993 .

[49]  J. Hautvast,et al.  Bioavailability of lutein from vegetables is 5 times higher than that of beta-carotene. , 1999, The American journal of clinical nutrition.

[50]  M. Boekel CRFSFS : Comprehensive Reviews in Food Science and Food Safety , 2008 .

[51]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.

[52]  C. Rock,et al.  Bioavailability of beta-carotene is lower in raw than in processed carrots and spinach in women. , 1998, The Journal of nutrition.

[53]  J. Castenmiller,et al.  Bioavailability and bioconversion of carotenoids. , 1998, Annual review of nutrition.

[54]  Linda C Tapsell,et al.  Food, not nutrients, is the fundamental unit in nutrition. , 2007, Nutrition reviews.

[55]  T. Suortti,et al.  Molecular weight distribution, measured by HPSEC, and viscosity of water-soluble dietary fiber in carrots following different types of processing , 1995 .

[56]  R. Faulks,et al.  Challenges to understanding and measuring carotenoid bioavailability. , 2005, Biochimica et biophysica acta.

[57]  S. Tanumihardjo Food-based approcahes for ensuring adequate vitamin A nutrition. , 2008 .

[58]  B. Bowman,et al.  Serum retinol distributions in residents of the United States: third National Health and Nutrition Examination Survey, 1988-1994. , 2001, The American journal of clinical nutrition.

[59]  J. Olson,et al.  Vitamin A and vitamin E statuses of preschool children of socioeconomically disadvantaged families living in the midwestern United States , 1997, European Journal of Clinical Nutrition.

[60]  B. Rao,et al.  Absorption of dietary carotenes in human subjects. , 1970, The American journal of clinical nutrition.

[61]  G. Rechkemmer,et al.  Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich foods , 1999, European journal of nutrition.

[62]  Lars Bode,et al.  Poverty, obesity, and malnutrition: an international perspective recognizing the paradox. , 2007, Journal of the American Dietetic Association.

[63]  R. Carle,et al.  Antiproliferation activity and metabolism of black carrot anthocyanins , 2007 .

[64]  P. Simon,et al.  Carotenes in typical and dark orange carrots , 1987 .

[65]  P. Simon,et al.  Beta-carotene from red carrot maintains vitamin A status, but lycopene bioavailability is lower relative to tomato paste in Mongolian gerbils. , 2007, The Journal of nutrition.

[66]  P. Simon,et al.  Biofortified carrot intake enhances liver antioxidant capacity and vitamin a status in mongolian gerbils. , 2008, The Journal of nutrition.

[67]  K. Gould,et al.  Nature's Swiss Army Knife: The Diverse Protective Roles of Anthocyanins in Leaves , 2004, Journal of biomedicine & biotechnology.

[68]  A. Edwards,et al.  Alpha- and beta-carotene from a commercial puree are more bioavailable to humans than from boiled-mashed carrots, as determined using an extrinsic stable isotope reference method. , 2002, The Journal of nutrition.

[69]  S. Tanumihardjo,et al.  Vitamin A in dietary supplements and fortified foods: too much of a good thing? , 2003, Journal of the American Dietetic Association.

[70]  M. Nyman,et al.  Dose-response effects of boiled carrots and effects of carrots in lactic acid in mixed meals on glycaemic response and satiety. , 1994, European journal of clinical nutrition.

[71]  R. Carle,et al.  Quantification of anthocyanins in black carrot extracts (Daucus carota ssp. sativus var. atrorubens Alef.) and evaluation of their color properties , 2004 .

[72]  N. Chiewchan,et al.  Production of antioxidant high dietary fiber powder from carrot peels , 2008 .

[73]  J. Marlett Content and composition of dietary fiber in 117 frequently consumed foods. , 1992, Journal of the American Dietetic Association.

[74]  P. Simon,et al.  Lycopene and β-carotene are bioavailable from lycopene ‘red’ carrots in humans , 2004, European Journal of Clinical Nutrition.

[75]  W. Vach,et al.  Inhibitory effects of feeding with carrots or (-)-falcarinol on development of azoxymethane-induced preneoplastic lesions in the rat colon. , 2005, Journal of agricultural and food chemistry.

[76]  B. Rani,et al.  Fibre constituents of some foods , 1994, Plant foods for human nutrition.

[77]  P. Finglas,et al.  Interlaboratory studies of HPLC procedures for the analysis of carotenoids in foods , 1996 .

[78]  K. Waldron,et al.  Characterization and distribution of phenolics in carrot cell walls. , 2008, Journal of agricultural and food chemistry.

[79]  Elizabeth J Johnson,et al.  Carotenoid actions and their relation to health and disease. , 2005, Molecular aspects of medicine.

[80]  R. Atkinson,et al.  Strategies to Increase Vegetable or Reduce Energy and Fat Intake Induce Weight Loss in Adults , 2009, Experimental biology and medicine.

[81]  P. Simon,et al.  Antioxidant phytochemicals and antioxidant capacity of biofortified carrots (Daucus carota L.) of various colors. , 2009, Journal of agricultural and food chemistry.

[82]  G. Beecher,et al.  Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption. , 2006, Journal of agricultural and food chemistry.

[83]  N. Binkley,et al.  Serum carotenoid concentrations in postmenopausal women from the United States with and without osteoporosis. , 2008, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[84]  G. Mazza,et al.  Anthocyanins exist in the circulation primarily as metabolites in adult men. , 2005, The Journal of nutrition.

[85]  L. P. Christensen,et al.  Bioactivity of falcarinol and the influenceof processing and storage on its content in carrots (Daucus carota L) , 2003 .

[86]  H. Lindegaard,et al.  [Carotinaemia in patient with excessive beta-carotene food-intake and dysregulated diabetes mellitus]. , 2009, Ugeskrift for laeger.

[87]  L. Yonekura,et al.  Intestinal absorption of dietary carotenoids. , 2007, Molecular nutrition & food research.

[88]  B. Bowman,et al.  Intraindividual variation in serum retinol concentrations among participants in the third National Health and Nutrition Examination Survey, 1988-1994. , 2004, The American journal of clinical nutrition.

[89]  P. Simon,et al.  Carrots and Related Vegetable Umbelliferae , 1999 .

[90]  Lars Porskjær Christensen,et al.  Health promoting compounds in vegetables and fruits: A systematic approach for identifying plant components with impact on human health , 2004 .

[91]  Catherine M Champagne,et al.  The evidence for dietary prevention and treatment of cardiovascular disease. , 2008, Journal of the American Dietetic Association.

[92]  T. Nishiyama,et al.  Induction of antioxidant and phase 2 drug-metabolizing enzymes by falcarindiol isolated from Notopterygium incisum extract, which activates the Nrf2/ARE pathway, leads to cytoprotection against oxidative and electrophilic stress. , 2009, Archives of biochemistry and biophysics.

[93]  W. Schnitzler,et al.  Evaluation of different coloured carrot cultivars on antioxidative capacity based on their carotenoid and phenolic contents , 2007, International journal of food sciences and nutrition.

[94]  Hartwig Schulz,et al.  In situ simultaneous analysis of polyacetylenes, carotenoids and polysaccharides in carrot roots. , 2005, Journal of agricultural and food chemistry.

[95]  Jian-Hua Fu,et al.  Greater vegetable and fruit intake is associated with a lower risk of breast cancer among Chinese women , 2009, International journal of cancer.

[96]  P. Simon,et al.  Lutein and beta-carotene from lutein-containing yellow carrots are bioavailable in humans. , 2004, The American journal of clinical nutrition.

[97]  B. Clevidence,et al.  Role of Color and Pigments in Breeding, Genetics, and Nutritional Improvement of Carrots , 2008 .

[98]  L. P. Christensen,et al.  Bioactive polyacetylenes in food plants of the Apiaceae family: occurrence, bioactivity and analysis. , 2006, Journal of pharmaceutical and biomedical analysis.

[99]  M. Parker,et al.  Solubilization of carotenoids from carrot juice and spinach in lipid phases: I. Modeling the gastric lumen , 2003, Lipids.

[100]  R. Shrestha,et al.  Recovery from impaired dark adaptation in nightblind pregnant Nepali women who receive small daily doses of vitamin A as amaranth leaves, carrots, goat liver, vitamin A-fortified rice, or retinyl palmitate. , 2005, The American journal of clinical nutrition.

[101]  De-Xing Hou,et al.  Potential mechanisms of cancer chemoprevention by anthocyanins. , 2003, Current molecular medicine.

[102]  P. A. van den Brandt,et al.  Intakes of Fruit, Vegetables, and Carotenoids and Renal Cell Cancer Risk: A Pooled Analysis of 13 Prospective Studies , 2009, Cancer Epidemiology Biomarkers & Prevention.

[103]  Jeana Gross,et al.  Pigments in Vegetables: Chlorophylls and Carotenoids , 1995 .

[104]  Tiina H Rissanen,et al.  Carotenoids and cardiovascular health. , 2006, The American journal of clinical nutrition.

[105]  Otto Hänninen,et al.  Serum β-carotene response to supplementation with raw carrots, carrot juice or purified β-carotene in healthy non-smoking women , 1996 .

[106]  S. Tanumihardjo,et al.  Biofortification of Staple Crops: An Emerging Strategy to Combat Hidden Hunger. , 2008 .

[107]  Augustin Scalbert,et al.  Effect of carrot intake on cholesterol metabolism and on antioxidant status in cholesterol-fed rat , 2003, European journal of nutrition.

[108]  M. A. Vieira,et al.  CHEMICAL, PHYSICAL AND SENSORY PARAMETERS OF DIFFERENT CARROT VARIETIES (DAUCUS CAROTA L.) , 2007 .

[109]  P. Simon,et al.  High Carotene Mass Carrot Population , 1989, HortScience.

[110]  A. Fillery-Travis,et al.  Solubilization of carotenoids from carrot juice and spinach in lipid phases: II. Modeling the duodenal environment , 2003, Lipids.

[111]  Sonia S Anand,et al.  A systematic review of the evidence supporting a causal link between dietary factors and coronary heart disease. , 2009, Archives of internal medicine.

[112]  L. Howard,et al.  Processing Effects on Carrot Phytonutrients , 2006 .

[113]  E. Frazão,et al.  How Much Do Americans Pay for Fruits and Vegetables , 2004 .

[114]  P. Simon,et al.  B7262, purple carrot inbred , 1997 .

[115]  R. Greil,et al.  Polyacetylenes from the Apiaceae vegetables carrot, celery, fennel, parsley, and parsnip and their cytotoxic activities. , 2005, Journal of agricultural and food chemistry.

[116]  Michael A Grusak,et al.  Spinach or carrots can supply significant amounts of vitamin A as assessed by feeding with intrinsically deuterated vegetables. , 2005, The American journal of clinical nutrition.

[117]  She-Ching Wu,et al.  Physicochemical changes upon micronization process positively improve the intestinal health-enhancement ability of carrot insoluble fibre , 2007 .

[118]  Jialiang Li,et al.  Lutein absorption is facilitated with cosupplementation of ascorbic acid in young adults. , 2005, Journal of the American Dietetic Association.

[119]  Reinhold Carle,et al.  Detection of peonidin and pelargonidin glycosides in black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) by high-performance liquid chromatography/electrospray ionization mass spectrometry. , 2003, Rapid communications in mass spectrometry : RCM.

[120]  P. Simon,et al.  Carotenoid profiles and consumer sensory evaluation of specialty carrots (Daucus carota, L.) of various colors. , 2004, Journal of agricultural and food chemistry.

[121]  D. Bell,et al.  Direct vasoactive and vasoprotective properties of anthocyanin-rich extracts. , 2006, Journal of applied physiology.

[122]  J. Potter,et al.  Plasma carotenoids as biomarkers of vegetable and fruit intake. , 1994, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[123]  P. Trumbo,et al.  Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. , 1998, Journal of the American Dietetic Association.

[124]  S B Roberts,et al.  Dietary fiber and weight regulation. , 2009, Nutrition reviews.

[125]  P. Simon DOMESTICATION, HISTORICAL DEVELOPMENT, AND MODERN BREEDING OF CARROT , 2000 .

[126]  B. Chew,et al.  Carotenoid action on the immune response. , 2004, The Journal of nutrition.

[127]  J. Erdman,et al.  Isolation and partial characterization of .alpha.- and .beta.-carotene-containing carotenoprotein from carrot (Daucus carota L.) root chromoplasts , 1992 .

[128]  G. Rechkemmer,et al.  Moderate intervention with carotenoid-rich vegetable products reduces lipid peroxidation in men. , 2000, The Journal of nutrition.

[129]  C. Chau,et al.  Carrot insoluble fiber-rich fraction lowers lipid and cholesterol absorption in hamsters , 2006 .

[130]  P. Simon,et al.  Bioavailability of β-carotene (βC) from purple carrots is the same as typical orange carrots while high-βC carrots increase βC stores in Mongolian gerbils(Meriones unguiculatus) , 2006, British Journal of Nutrition.

[131]  Thomas,et al.  Fibre-mediated physiological effects of raw and processed carrots in humans , 2005 .

[132]  J. Bertram,et al.  Cancer prevention by retinoids and carotenoids: independent action on a common target. , 2005, Biochimica et biophysica acta.

[133]  E. Ponce,et al.  World Cancer Research Fund, American Institute for Cancer Research. Second Expert Report, Food, Nutrition, Physical Activity and the Prevention of Cancer: A Global Perspective. United Kingdom: WCRF/AICR, 2001 , 2009 .

[134]  J. Erdman,et al.  Reversed phase HPLC analysis of α- and β-carotene from selected raw and cooked vegetables , 1988 .

[135]  B. Frei,et al.  Coffee and Health: A Review of Recent Human Research , 2006, Critical reviews in food science and nutrition.

[136]  G. Rechkemmer,et al.  Supplementation of a diet low in carotenoids with tomato or carrot juice does not affect lipid peroxidation in plasma and feces of healthy men. , 2004, The Journal of nutrition.

[137]  T. Greiner,et al.  Supplementing lactating women with puréed papaya and grated carrots improved vitamin A status in a placebo-controlled trial. , 2001, The Journal of nutrition.

[138]  J. C. Smith,et al.  Plasma carotenoid response to chronic intake of selected foods and beta-carotene supplements in men. , 1992, The American journal of clinical nutrition.

[139]  R. Ziegler,et al.  Seasonal variation in intake of carotenoids and vegetables and fruits among white men in New Jersey. , 1987, The American journal of clinical nutrition.

[140]  J. Gross Pigments in Vegetables , 1991, Springer US.

[141]  S. Ripatti,et al.  Randomised trial of α-tocopherol and β-carotene supplements on incidence of major coronary events in men with previous myocardial infarction , 1997, The Lancet.

[142]  J. Bulux,et al.  The plasma beta-carotene response to a single meal of carrots in Guatemalan schoolchildren. , 1998, International journal of food sciences and nutrition.

[143]  K. Gould,et al.  Anthocyanins in leaves: light attenuators or antioxidants? , 2003, Functional plant biology : FPB.

[144]  E. Rock,et al.  Genetic Variability Influences Carotenoid, Vitamin, Phenolic, and Mineral Content in White, Yellow, Purple, Orange, and Dark-orange Carrot Cultivars , 2004 .

[145]  Gary Williamson,et al.  Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. , 2005, The American journal of clinical nutrition.

[146]  J. Erdman,et al.  The crystalline form of carotenes and the food matrix in carrot root decrease the relative bioavailability of beta- and alpha-carotene in the ferret model. , 1996, Journal of the American College of Nutrition.

[147]  M. Clifford,et al.  Dietary phenolics: chemistry, bioavailability and effects on health. , 2009, Natural product reports.

[148]  Joint Fao,et al.  Human energy requirements : report of a Joint FAO/WHO/UNU Expert Consultation : Rome, 17-24 October 2001 , 2004 .

[149]  J. Olson Recommended dietary intakes (RDI) of vitamin A in humans. , 1987, The American journal of clinical nutrition.

[150]  M. Nyman,et al.  Influence of processing and cooking of carrots in mixed meals on satiety, glucose and hormonal response. , 1995, International journal of food sciences and nutrition.

[151]  G. Stoner,et al.  Anthocyanins and their role in cancer prevention. , 2008, Cancer letters.

[152]  A. Edwards,et al.  Bioavailability of carotenoids in human subjects , 1999, Proceedings of the Nutrition Society.

[153]  P. Hollman,et al.  Chlorogenic acid, quercetin-3-rutinoside and black tea phenols are extensively metabolized in humans. , 2003, The Journal of nutrition.

[154]  J. Erdman,et al.  Isolation and partial characterization of an 18 kDa carotenoid-protein complex from carrot roots , 1994 .

[155]  L. Holmberg,et al.  Excessive Dietary Intake of Vitamin A Is Associated with Reduced Bone Mineral Density and Increased Risk for Hip Fracture , 1998, Annals of Internal Medicine.

[156]  C. Chau,et al.  Comparison of the characteristics, functional properties, and in vitro hypoglycemic effects of various carrot insoluble fiber-rich fractions , 2004 .

[157]  Christophe Nguyen-The,et al.  Changes in Phenolic Content in Fresh Ready-to-use Shredded Carrots during Storage , 1993 .

[158]  S. Tanumihardjo,et al.  CAROTENOIDS | Epidemiology of Health Effects , 2005 .

[159]  Organización Mundial de la Salud Global prevalence of vitamin A deficiency , 1995 .

[160]  廣畑 富雄,et al.  Food, Nutrition, Physical Activity, and the Prevention of Cancer: a Global Perspective , 2007 .

[161]  E. Giovannucci A Review of Epidemiologic Studies of Tomatoes, Lycopene, and Prostate Cancer , 2002, Experimental biology and medicine.

[162]  E. Barrett-Connor,et al.  Retinol Intake and Bone Mineral Density in the Elderly: The Rancho Bernardo Study , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[163]  Joint Fao Carbohydrates in human nutrition : report of a joint FAO/WHO expert consultation, Rome, 14-18 April 1997 , 1998 .

[164]  F. Shahidi,et al.  Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. , 2001, Journal of agricultural and food chemistry.

[165]  A. Thybo,et al.  The role of volatile compounds on aroma and flavour perception in coloured raw carrot genotypes , 2008 .

[166]  E. Feskens,et al.  Both α- and β-Carotene, but Not Tocopherols and Vitamin C, Are Inversely Related to 15-Year Cardiovascular Mortality in Dutch Elderly Men , 2008 .

[167]  G. Rechkemmer,et al.  Effects of carrot and tomato juice consumption on faecal markers relevant to colon carcinogenesis in humans , 2008, British Journal of Nutrition.

[168]  Julia A. Ello-Martin,et al.  Dietary energy density in the treatment of obesity: a year-long trial comparing 2 weight-loss diets. , 2007, The American journal of clinical nutrition.

[169]  C. Chau,et al.  Influence of insoluble fiber fractions from carambola and carrot on intestinal enzymes and fecal bacterial enzymes in hamsters , 2005 .

[170]  V. Wray,et al.  Anthocyanins from cell suspension cultures of Daucus carota. , 1992, Phytochemistry.

[171]  R. Reifen,et al.  β-carotene bioavailability from differently processed carrot meals in human ileostomy volunteers , 2003, European journal of nutrition.

[172]  P. Simon,et al.  Serum α- and β-Carotene Concentrations Qualitatively Respond to Sustained Carrot Feeding , 2009, Experimental biology and medicine.

[173]  L. Pollak,et al.  Plant Breeding for Human Nutritional Quality , 2009 .

[174]  J. Olson,et al.  Recommended dietary intakes (RDI) of vitamin K in humans. , 1987, The American journal of clinical nutrition.

[175]  L. P. Christensen,et al.  Determination of polyacetylenes in carrot roots (Daucus carota L.) by high-performance liquid chromatography coupled with diode array detection. , 2007, Journal of separation science.

[176]  Véra Lucia,et al.  Polyphenol, Ascorbic Acid and Total Carotenoid Contents in Common Fruits and Vegetables Teores de Polifenóis, Ácido Ascórbico e Carotenóides Totais em Frutas e Hortaliças Usualmente Consumidas , 2006 .

[177]  G. Yen,et al.  Antioxidant and cognitive promotion effects of anthocyanin-rich mulberry (Morus atropurpurea L.) on senescence-accelerated mice and prevention of Alzheimer's disease. , 2010, The Journal of nutritional biochemistry.

[178]  R. Parker Absorption, metabolism, and transport of carotenoids , 1996, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[179]  A. Gallacher,et al.  ISOLATION AND PARTIAL CHARACTERIZATION , 1976 .

[180]  B. Clevidence,et al.  Plasma and urine responses are lower for acylated vs nonacylated anthocyanins from raw and cooked purple carrots. , 2005, Journal of agricultural and food chemistry.

[181]  F. Granado,et al.  Bioavailability of α-+β-carotene in Type 1 diabetic patients , 2001 .

[182]  A. Rao,et al.  Carotenoids and human health. , 2007, Pharmacological research.

[183]  Cesarettin Alasalvar,et al.  Method for the static headspace analysis of carrot volatiles , 1999 .

[184]  J. C. Smith,et al.  Identification, quantification, and relative concentrations of carotenoids and their metabolites in human milk and serum. , 1997, Analytical chemistry.

[185]  Julia A. Ello-Martin,et al.  What can intervention studies tell us about the relationship between fruit and vegetable consumption and weight management? , 2004, Nutrition reviews.

[186]  P. Simon Carrots and Other Horticultural Crops as a Source of Provitamin A Carotenes , 1990 .

[187]  J. C. Smith,et al.  Plasma carotenoids in normal men after a single ingestion of vegetables or purified beta-carotene. , 1989, The American journal of clinical nutrition.

[188]  J. Cummings,et al.  Hypocholesterolemic action of dietary fiber unrelated to fecal bulking effect. , 1979, The American journal of clinical nutrition.

[189]  R. Andersson,et al.  Effects of Boiling and Storage on Dietary Fibre and Digestible Carbohydrates in Various Cultivars of Carrots , 1997 .

[190]  Elio Riboli,et al.  Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk. , 2003, The American journal of clinical nutrition.

[191]  M. Heinonen Carotenoids and provitamin A activity of carrot (Daucus carota L.) cultivars , 1990 .