Volumetric Bar-Chart Chips for Biosensing.

The volumetric bar-chart chip (V-Chip) is a microfluidics-based, point-of-care (POC) device for the multiplexed and quantitative measurement of biomarkers. Volumetric readouts, based on the measurement of oxygen generated by a reaction between catalase and hydrogen peroxide, allow instant visual quantitation of target biomarkers and provide visualized bar charts without any assistance from instruments and without the need for data processing or graphics plotting. V-Chip shows potential capabilities in POC and personalized diagnostics; for instance, it can be utilized for making high-throughput, multiplexed, and quantitative measurements. Further, this system is highly portable and can be performed at low cost. The development of the V-Chip thus marks a POC milestone and opens up the possibility of instrument-free personalized diagnostics. Here, we describe the protocols for the fabrication of V-Chip and the use of silica nanoparticles as the probe carrier for the V-Chip-based enzyme-linked immunosorbent assay (ELISA) for the detection of biomarkers.

[1]  Lidong Qin,et al.  Self-powered microfluidic chips for multiplexed protein assays from whole blood. , 2009, Lab on a chip.

[2]  Y. K. Cheung,et al.  1 Supplementary Information for : Microfluidics-based diagnostics of infectious diseases in the developing world , 2011 .

[3]  L. Gervais,et al.  Microfluidic Chips for Point‐of‐Care Immunodiagnostics , 2011, Advanced materials.

[4]  David J. Mooney,et al.  Label-free biomarker detection from whole blood , 2009, 2010 10th IEEE International Conference on Solid-State and Integrated Circuit Technology.

[5]  Yujun Song,et al.  Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer. , 2016, ACS nano.

[6]  Mauro Ferrari,et al.  Point-of-care technologies for molecular diagnostics using a drop of blood. , 2014, Trends in biotechnology.

[7]  Yi Lu,et al.  Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. , 2011, Nature chemistry.

[8]  Youli Zu,et al.  Multiplexed volumetric bar-chart chip for point-of-care diagnostics , 2012, Nature Communications.

[9]  Samuel K Sia,et al.  Actuation of elastomeric microvalves in point-of-care settings using handheld, battery-powered instrumentation. , 2010, Lab on a chip.

[10]  Rustem F Ismagilov,et al.  Digital isothermal quantification of nucleic acids via simultaneous chemical initiation of recombinase polymerase amplification reactions on SlipChip. , 2011, Analytical chemistry.

[11]  Ping Wang,et al.  Integration of platinum nanoparticles with a volumetric bar-chart chip for biomarker assays. , 2014, Angewandte Chemie.

[12]  Anupam Singhal,et al.  Megapixel digital PCR , 2011, Nature Methods.

[13]  Yi Guo,et al.  Competitive volumetric bar-chart chip with real-time internal control for point-of-care diagnostics. , 2015, Analytical chemistry.

[14]  Rustem F Ismagilov,et al.  Nanoliter multiplex PCR arrays on a SlipChip. , 2010, Analytical chemistry.

[15]  B. Duan,et al.  3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. , 2013, Journal of biomedical materials research. Part A.

[16]  Liang Li,et al.  User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments. , 2010, Journal of the American Chemical Society.

[17]  Detlev Belder.  Screening in one sweep using the slipchip. , 2010, Angewandte Chemie.

[18]  Lidong Qin,et al.  A multistage volumetric bar chart chip for visualized quantification of DNA. , 2013, Journal of the American Chemical Society.

[19]  L. Hood,et al.  Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood , 2008, Nature Biotechnology.

[20]  Ying Li,et al.  A microfluidic platform with digital readout and ultra-low detection limit for quantitative point-of-care diagnostics. , 2015, Lab on a chip.

[21]  P. George,et al.  Reaction Between Catalase and Hydrogen Peroxide , 1947, Nature.

[22]  Chad A Mirkin,et al.  Large-area molecular patterning with polymer pen lithography , 2013, Nature Protocols.

[23]  Bing Sun,et al.  Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral load. , 2011, Journal of the American Chemical Society.