Counter-current chromatography: instrumentation, solvent selection and some recent applications to natural product purification

Abstract Modern instruments for counter-current chromatography (CCC) and centrifugal partition chromatography (CPC) are reviewed, and a description of biphasic solvent systems is provided, together with some simple rules for finding the best system for a given purification. Applications include the purification of acetogenins, 10-deacetyl-baccatin III, amphotericin B, anthocyanins, hop bitter acids, alkaloids and partially depolymerized fucans. CPC–NMR coupling is briefly discussed.

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