Schizosaccharomyces pombe Produces Novel Pyruvate-containing N-Linked Oligosaccharides*

The large N-linked oligosaccharides released by endo-β-N-acetylglucosaminidase H from Schizosaccharomyces pombe glycoproteins were analyzed for the presence of noncarbohydrate functional groups. No phosphate, sulfate, or acetate could be detected; however, approximately six molecules of pyruvic acid/molecule were found on 98% of the oligosaccharides. Pyruvate moieties were acetal (ketal)-linked to galactose residues in the R configuration to carbons 4 and 6. This is the first report of pyruvate functional groups being attached to Nlinked oligosaccharides in yeast and appears only to be the second documentation of this sugar modification in eukaryotes.

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