Do we have the auxin receptor yet

Several auxin-binding proteins (ABP) have now been identified using a variety of techniques. A 43-kDa glycoprotein thought to be a dimer of 22-kDa subunits has been identified as a strong candidate for the auxin receptor that mediates cell elongation in etiolated maize shoots. The primary sequence has been deduced and several interesting structural features have been discerned. There is indirect evidence that this 22-kDa ABP has a receptor function, the most compelling being that antibodies directed against the ABP can block an auxin-induced response. There is evidence that changes in auxin-induced growth capacity in shoots correlates with changes in the abundance of the 22-kDa ABP suggesting that in some cases the 22-kDa ABP may be limiting growth. Confirmation of receptor function for one of these newly-identified ABP's should open the way for genetic manipulation of crop growth.

[1]  G. Ephritikhine,et al.  Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[2]  S. Munro,et al.  A C-terminal signal prevents secretion of luminal ER proteins , 1987, Cell.

[3]  M. Kikuchi,et al.  Modulation of Gene Expression in Isolated Nuclei by Auxin-Binding Proteins , 1989 .

[4]  A. Jones,et al.  Photoaffinity labeling of indole-3-acetic acid-binding proteins in maize. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[5]  P. Ray,et al.  Specificity of Auxin-binding Sites on Maize Coleoptile Membranes as Possible Receptor Sites for Auxin Action. , 1977, Plant physiology.

[6]  R. Palmiter,et al.  Genetic engineering of livestock. , 1989, Science.

[7]  J. Vandekerckhove,et al.  Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin. , 1989, The EMBO journal.

[8]  U. Kutschera,et al.  Differential effect of auxin on in vivo extensibility of cortical cylinder and epidermis in pea internodes. , 1987, Plant physiology.

[9]  P. Ray.  Auxin-binding Sites of Maize Coleoptiles Are Localized on Membranes of the Endoplasmic Reticulum. , 1977, Plant physiology.

[10]  N. Inohara,et al.  Auxin-binding protein located in the endoplasmic reticulum of maize shoots: molecular cloning and complete primary structure. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Walton,et al.  Evidence for Receptor Function of Auxin Binding Sites in Maize : RED LIGHT INHIBITION OF MESOCOTYL ELONGATION AND AUXIN BINDING. , 1981, Plant physiology.

[12]  R. Hertel.  The Mechanism of Auxin Transport as a Model for Auxin Action , 1983 .

[13]  G. Hicks,et al.  Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[14]  G. Hicks,et al.  The Diageotropica Mutant of Tomato Lacks High Specific Activity Auxin Binding Sites , 1989, Science.

[15]  R. Aloni.  The Induction of Vascular Tissues by Auxin , 1987 .

[16]  S. Sakai,et al.  Purification of an Auxin-Binding Protein from Etiolated Mung Bean Seedlings by Affinity Chromatography , 1983 .

[17]  A. Trewavas.  An auxin induces the appearance of auxin-binding activity in artichoke tubers , 1980 .

[18]  M. Venis.  Solubilisation and partial purification of auxin-binding sites of corn membranes , 1977, Nature.

[19]  M Löbler,et al.  Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). I. Purification by immunological methods and characterization. , 1985, The Journal of biological chemistry.

[20]  G. Koch,et al.  Perturbation of cellular calcium induces secretion of luminal ER proteins , 1989, Cell.

[21]  K. Palme,et al.  cDNA clones of the auxin‐binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods. , 1989, The EMBO journal.

[22]  M. Futai,et al.  Purification and properties of an auxin-binding protein from maize shoot membranes. , 1986, Journal of biochemistry.