Functionalized 2PP structures for the BioPhotonics Workstation

In its standard version, our BioPhotonics Workstation (BWS) can generate multiple controllable counter-propagating beams to create real-time user-programmable optical traps for stable three-dimensional control and manipulation of a plurality of particles. The combination of the platform with microstructures fabricated by two-photon polymerization (2PP) can lead to completely new methods to communicate with micro- and nano-sized objects in 3D and potentially open enormous possibilities in nano-biophotonics applications. In this work, we demonstrate that the structures can be used as microsensors on the BWS platform by functionalizing them with silica-based sol-gel materials inside which dyes can be entrapped.

[1]  R. Kopelman,et al.  Nitrite- and chloride-selective fluorescent nano-optodes and in vitro application to rat conceptuses. , 1998, Analytical chemistry.

[2]  A. Ashkin.  Acceleration and trapping of particles by radiation pressure , 1970 .

[3]  David Grosso,et al.  An optical fibre pH sensor based on dye doped mesostructured silica , 2004 .

[4]  Jesper Glückstad,et al.  Sorting particles with light , 2004, Nature materials.

[5]  J Bengtsson.  Kinoform design with an optimal-rotation-angle method. , 1994, Applied optics.

[6]  E. Isacoff,et al.  Scanless two-photon excitation of channelrhodopsin-2 , 2010, Nature Methods.

[7]  Pál Ormos,et al.  Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies. , 2009, Optics express.

[8]  Kurt Seiler,et al.  Design of a novel calcium-selective optode membrane based on neutral ionophores , 1990 .

[9]  Jeppe Seidelin Dam,et al.  Independent trapping, manipulation and characterization by an all-optical biophotonics workstation , 2008 .

[10]  Yoshio Hayasaki,et al.  Holographic femtosecond laser processing using optimal-rotation-angle method with compensation of spatial frequency response of liquid crystal spatial light modulator. , 2007, Applied optics.

[11]  Takashi Inoue,et al.  LCOS spatial light modulator controlled by 12-bit signals for optical phase-only modulation , 2007, SPIE OPTO.

[12]  Satoshi Kawata,et al.  Finer features for functional microdevices , 2001, Nature.

[13]  Mattias Goksör,et al.  Grid-free 3D multiple spot generation with an efficient single-plane FFT-based algorithm. , 2009, Optics express.

[14]  B. D. Mac Craith,et al.  Optical chemical sensors based on sol-gel materials: Recent advances and critical issues , 1997 .

[15]  Masaaki Sakakura,et al.  Parallel Drawing of Multiple Bent Optical Waveguides Using a Spatial Light Modulator , 2009 .

[16]  Peter John Rodrigo,et al.  Optical 3D Manipulation and Observation in Real-Time , 2006, J. Robotics Mechatronics.