Combination HCV core antigen and antibody assay on a fully automated chemiluminescence analyzer

BACKGROUND: HCV exposure among blood donors is serologically determined by detection of antibodies to HCV (anti‐HCV); however, the recent development of an assay for the detection of HCV core antigen identifies infection before anti‐HCV development. Simultaneous detection of HCV core antigen and anti‐HCV would shorten the window period before seroconversion over conventional HCV antibody screening assays.

[1]  C. Seed,et al.  Improved efficiency of national HIV, HCV, and HTLV antibody testing algorithms based on sequential screening immunoassays , 2003, Transfusion.

[2]  J. Pawlotsky.  Use and interpretation of virological tests for hepatitis C , 2002, Hepatology.

[3]  R. Tedder,et al.  Quantification of HCV RNA levels and detection of core antigen in donations before seroconversion , 2002, Transfusion.

[4]  J. Desenclos,et al.  Trends in residual risk of transfusion‐transmitted viral infections in France between 1992 and 2000 , 2002, Transfusion.

[5]  J. Löwer,et al.  Sensitivity of HCV core antigen and HCV RNA detection in the early infection phase , 2002, Transfusion.

[6]  A. Neumann,et al.  Clinical utility of total HCV core antigen quantification: A new indirect marker of HCV replication , 2002, Hepatology.

[7]  G. Leckie,et al.  Detection of HCV core antigen in human serum and plasma with an automated chemiluminescent immunoassay , 2002, Transfusion.

[8]  P. Holland,et al.  Fluctuation of serum HCV‐RNA levels in untreated blood donors with chronic hepatitis C virus infection , 2002, Journal of viral hepatitis.

[9]  F. Ansaldi,et al.  Novel Approach To Reduce the Hepatitis C Virus (HCV) Window Period: Clinical Evaluation of a New Enzyme-Linked Immunosorbent Assay for HCV Core Antigen , 2001, Journal of Clinical Microbiology.

[10]  S. Laperche,et al.  Efficacy of HCV core antigen detection during the preseroconversion period , 2000, Transfusion.

[11]  B. Caldwell,et al.  Detection of Hepatitis C Core Antigen in the Antibody Negative ‘Window’ Phase of Hepatitis C Infection , 2000, Vox Sanguinis.

[12]  S. Kleinman,et al.  Committee report. Nucleic acid amplification testing of blood donors for transfusion-transmitted infectious diseases: Report of the Interorganizational Task Force on Nucleic Acid Amplification Testing of Blood Donors. , 2000, Transfusion.

[13]  S. Yagi,et al.  Development of a Simple and Highly Sensitive Enzyme Immunoassay for Hepatitis C Virus Core Antigen , 1999, Journal of Clinical Microbiology.

[14]  H J Alter,et al.  An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. , 1990, Science.

[15]  Jules L. Dienstag,et al.  An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis , 1989 .