Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation

Abstract The antioxidant activity (ferric reducing/antioxidant power, FRAP) and oxygen-scavenging system of pulps during the fruit ripening and maturation were investigated in three cultivars of sweet orange ( Citrus sinensis (L.) Osbeck). The highest FRAP values and activities of antioxidant enzymes and contents of nonenzymes were detected in ‘Red Flesh’ navel orange. The activities of oxygen-scavenging enzymes, superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (G-POD), decreased with ripening and maturation of fruit. Ascorbate peroxidase (AsA-POD) and dehydroascorbate reductase (DHAR) activities were also stably declined, whereas glutathione reductase (GR) activity and nonenzymes in the ascorbate–glutathione cycle displayed a single-peak pattern which paralleled the significant changes in FRAP values. In addition, the ratios of ascorbate/dehydroascorbate (AsA/DHAsA), reduced glutathione/oxidized glutathione (GSH/GSSG) were also decreased. Changes in the activities of antioxidant enzymes and the contents of nonenzymes during ripening indicated that the antioxidant system plays a fundamental role in the ripening of orange fruits.

[1]  W. Bramlage,et al.  Superoxide Dismutase Activities in Senescing Apple Fruit (Malus domestica Borkh.) , 1994 .

[2]  F. J. Corpas,et al.  Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. , 2001, Trends in plant science.

[3]  E. Lissi,et al.  Evaluation of total reactive antioxidant potential (TRAP) of tissue homogenates and their cytosols. , 2001, Archives of biochemistry and biophysics.

[4]  C. Rice-Evans,et al.  Structure-antioxidant activity relationships of flavonoids and phenolic acids. , 1996, Free radical biology & medicine.

[5]  C. Berset,et al.  Antioxidant activity and phenolic composition of citrus peel and seed extracts , 1998 .

[6]  P. Mullineaux,et al.  The presence of dehydroascorbate and dehydroascorbate reductase in plant tissues , 1998, FEBS letters.

[7]  R. Larson.  The antioxidants of higher plants , 1988 .

[8]  A. Rychter,et al.  Activity of Enzymes Involved in the Turnover of Hydrogen Peroxide during Fruit Senescence , 1979, Botanical Gazette.

[9]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[10]  R. Crawford,et al.  Superoxide Dismutase as an Anaerobic Polypeptide : A Key Factor in Recovery from Oxygen Deprivation in Iris pseudacorus? , 1987, Plant physiology.

[11]  K. Asada,et al.  THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons. , 1999, Annual review of plant physiology and plant molecular biology.

[12]  R. Prior,et al.  Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. , 2001, Journal of agricultural and food chemistry.

[13]  S. Gepstein,et al.  Abundance of the Major Chloroplast Polypeptides during Development and Ripening of Tomato Fruits: An Immunological Study. , 1988, Plant physiology.

[14]  R. Prior,et al.  Measurement of oxygen radical absorbance capacity in biological samples. , 1999, Methods in enzymology.

[15]  J. F. Ayala-Zavala,et al.  Effect of storage temperatures on antioxidant capacity and aroma compounds in strawberry fruit , 2004 .

[16]  D. Ziegler.  Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation. , 1985, Annual review of biochemistry.

[17]  M. Kar,et al.  Catalase, Peroxidase, and Polyphenoloxidase Activities during Rice Leaf Senescence. , 1976, Plant physiology.

[18]  F. J. Corpas,et al.  The activated oxygen role of peroxisomes in senescence , 1998, Plant physiology.

[19]  G. A. Hamilton.  10 – CHEMICAL MODELS AND MECHANISMS FOR OXYGENASES , 1974 .

[20]  I. K. Smith,et al.  Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithiobis(2-nitrobenzoic acid). , 1988, Analytical biochemistry.

[21]  S. Y. Wang,et al.  Changes in oxygen-scavenging systems and membrane lipid peroxidation during maturation and ripening in blackberry. , 2001, Journal of agricultural and food chemistry.

[22]  Freya Q. Schafer,et al.  Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. , 2001, Free radical biology & medicine.

[23]  H. Deigner,et al.  Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U‐937 macrophage‐like cells , 1996, FEBS letters.

[24]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[25]  S. Rogiers,et al.  Maturation and Ripening of Fruit of Amelanchier alnifolia Nutt. are Accompanied by Increasing Oxidative Stress , 1998 .

[26]  S. Kondo,et al.  Preharvest antioxidant activities of tropical fruit and the effect of low temperature storage on antioxidants and jasmonates , 2005 .

[27]  J. Sala.  Involvement of oxidative stress in chilling injury in cold-stored mandarin fruits , 1998 .

[28]  M. Lucas,et al.  Antioxidant Response to NaCl Stress in a Control and an NaCl-Tolerant Cotton Cell Line Grown in the Presence of Paraquat, Buthionine Sulfoximine, and Exogenous Glutathione , 1996, Plant physiology.

[29]  S. Shigeoka,et al.  Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase. , 1980, The Biochemical journal.

[30]  K. Asada,et al.  Separate Assays Specific for Ascorbate Peroxidase and Guaiacol Peroxidase and for the Chloroplastic and Cytosolic Isozymes of Ascorbate Peroxidase in Plants , 1994 .

[31]  H. Greppin,et al.  Extracellular ascorbic acid and enzyme activities related to ascorbic acid metabolism in Sedum album L. leaves after ozone exposure , 1988 .

[32]  R. Perl-Treves,et al.  The tomato Cu,Zn superoxide dismutase genes are developmentally regulated and respond to light and stress , 1991, Plant Molecular Biology.

[33]  G. Pastori,et al.  Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves , 1998, Plant physiology.

[34]  H. Schreiber,et al.  Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum , 1995, FEBS letters.

[35]  Shaohua Li,et al.  Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage , 2006 .

[36]  J. Hernández,et al.  Mitochondrial and peroxisomal ascorbate peroxidase of pea leaves , 1998 .

[37]  R. Legge,et al.  THE ROLE OF FREE RADICALS IN SENESCENCE AND WOUNDING. , 1987, The New phytologist.

[38]  N. R. Knowles,et al.  Changes in Lipid Peroxidation and Lipolytic and Free-Radical Scavenging Enzyme Activities during Aging and Sprouting of Potato (Solanum tuberosum) Seed-Tubers , 1993, Plant physiology.

[39]  P. Mullineaux,et al.  Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening , 2002, Planta.

[40]  C. Foyer,et al.  ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control. , 1998, Annual review of plant physiology and plant molecular biology.

[41]  C. Frenkél,et al.  Involvement of hydrogen peroxide in the regulation of senescence in pear. , 1977, Plant physiology.

[42]  W. Jongen,et al.  Activity and concentration of polyphenolic antioxidants in apple: effect of cultivar, harvest year, and storage conditions. , 2001, Journal of agricultural and food chemistry.

[43]  R. Dhindsa,et al.  Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase , 1981 .