Cytometric validation of immunocytochemical observations in developing lung cancer

Video‐enhanced optical microscopy objectively confirms that immunocytochemical biomarker supplementation of routine cytology permits separation of (1) “pre”‐cancer epithelial cells from JHLP screened individuals who subsequently developed lung cancer from (2) similarly atypical epithelial cells from JHLP participants who did not. The establishment of a bank of sputum cytology specimens along with a data respository of 5‐yr follow‐up and clinical outcome had permitted banked specimens to be immunostained and scored against a gold standard of subsequent histologic lung cancer. A highly skilled cytopathologist, interpreting immunostained screening sputum specimens and blinded to the clinical outcome, originally was able to correctly predict clinical outcome with 88.7% accuracy compared to this “gold standard” (Tockman et al. J Clin Oncol 1988;6:1685‐1693). This study presents an independent confirmation of that earlier immunocytochemical classification through feature extraction of digitally recorded, transmission optical microscope video images of immunostained, moderately atypical sputum cells from the original slides.

[1]  W. C. Ball,et al.  Early lung cancer detection: results of the initial (prevalence) radiologic and cytologic screening in the Johns Hopkins study. , 2015, The American review of respiratory disease.

[2]  H. Antoniades,et al.  Malignant epithelial cells in primary human lung carcinomas coexpress in vivo platelet-derived growth factor (PDGF) and PDGF receptor mRNAs and their protein products. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[3]  N. J. Pressman,et al.  Considerations in bringing a cancer biomarker to clinical application. , 1992, Cancer research.

[4]  K. Kinzler,et al.  Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. , 1992, Science.

[5]  P. Herrlich,et al.  Interference of pathway specific transcription factors. , 1992, Biochimica et biophysica acta.

[6]  M. Selman,et al.  Platelet-derived growth factor in idiopathic pulmonary fibrosis. , 1990, The Journal of clinical investigation.

[7]  M. Sporn,et al.  Autoinduction of transforming growth factor beta 1 is mediated by the AP-1 complex , 1990, Molecular and cellular biology.

[8]  J. Mulshine,et al.  Antibody 624H12, which detects lung cancer at early stages, recognizes a sugar sequence in the glycosphingolipid difucosylneolactonorhexaosylceramide (V3FucIII3FunLc6Cer). , 1989, Archives of biochemistry and biophysics.

[9]  M. Birrer,et al.  A transfected L-myc gene can substitute for c-myc in blocking murine erythroleukemia differentiation , 1989, Molecular and cellular biology.

[10]  J. Minna,et al.  Deregulated expression of human c-jun transforms primary rat embryo cells in cooperation with an activated c-Ha-ras gene and transforms rat-1a cells as a single gene. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[11]  S. Hakomori.  Biochemical basis and clinical application of tumor-associated carbohydrate antigens: current trends and future perspectives. , 1989, Gan to kagaku ryoho. Cancer & chemotherapy.

[12]  M. Tockman,et al.  Sensitive and specific monoclonal antibody recognition of human lung cancer antigen on preserved sputum cells: a new approach to early lung cancer detection. , 1988, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[13]  R. Elashoff,et al.  Multiple markers for lung cancer diagnosis: validation of models for localized lung cancer. , 1988, Journal of the National Cancer Institute.

[14]  M Terada,et al.  Loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[15]  S. Rodenhuis,et al.  Mutational activation of the K-ras oncogene. A possible pathogenetic factor in adenocarcinoma of the lung. , 1987, The New England journal of medicine.

[16]  J. Minna,et al.  Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. , 1986, Cancer research.

[17]  A. Gazdar,et al.  Improved antigen detection in ethanol‐fixed cytologic specimens. A modified avidin‐biotin‐peroxidase complex (ABC) method , 1985, Diagnostic cytopathology.

[18]  S. Hakomori,et al.  Localization and alteration of mono-, di-, and trifucosyl alpha 1----3 type 2 chain structures during human embryogenesis and in human cancer , 1984, The Journal of experimental medicine.

[19]  J. Minna,et al.  Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies. , 1984, Cancer research.

[20]  J. Minna,et al.  Monoclonal antibodies that distinguish non-small cell from small cell lung cancer. , 1983, Journal of immunology.

[21]  M. Barbacid,et al.  Oncogenes in solid human tumours , 1982, Nature.

[22]  J. Minna,et al.  Monoclonal antibodies that demonstrate specificity for several types of human lung cancer. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[23]  O. Auerbach,et al.  Development of carcinoma of the lung as reflected in exfoliated cells , 1974, Cancer.

[24]  M. Tockman,et al.  Rational targets for the early detection of lung cancer. , 1992, Journal of the National Cancer Institute. Monographs.

[25]  Eun Sil Kim,et al.  Chest radiology. , 1992, Current opinion in radiology.

[26]  C. Harris,et al.  Oncogenes and tumor suppressor genes involved in human lung carcinogenesis. , 1990, Basic life sciences.