One-Step Fabrication of Droplet Arrays Using a Biomimetic Structural Chip.

In the field of one-step efficient preparation of dewetting droplet arrays, the process is hampered by the requirement for low chemical wettability of solid surfaces, which restricts the complete transition of wetting state and its broad prospects in biological applications. Inspired by the physical structure of the lotus leaf, enabling it to promote the change of the infiltration state of an aqueous solution on the surface, we developed a method of one-step fabrication of droplet arrays on the biomimetic structural chip designed in the present work. This greatly reduces the need for chemical modification techniques to achieve low wettability and reduces the reliance on complex and sophisticated surface preparation techniques, thus improving the fabrication efficiency of droplet arrays fully generated on a chip by one-step operation without the need for extra liquid phase or the control of harsh barometric pressure. We also studied the influence of dimensions of the biomimetic structure and the preparation process parameters such as number of smears and speed of smearing on the preparation rate and uniformity of the droplet arrays. The amplification of templating DNA molecules in the droplet arrays prepared in a one-step fabrication way is also performed to verify its application potential for DNA molecular diagnosis.

[1]  Yunlu Pan,et al.  Fouling‐Proof Cooling (FP‐Cool) Fabric Hybrid with Enhanced Sweat‐Elimination and Heat‐Dissipation for Personal Thermal Regulation , 2022, Advanced Functional Materials.

[2]  Yunlu Pan,et al.  Durable Superoleophobic Janus Fabric with Oil Repellence and Anisotropic Water-Transport Integration toward Energetic-Efficient Oil-Water Separation. , 2022, ACS applied materials & interfaces.

[3]  Hao Wang,et al.  Ultra-sensitive and rapid screening of acute myocardial infarction using 3D-affinity graphene biosensor , 2022, Cell Reports Physical Science.

[4]  Hsueh-Chia Chang,et al.  Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load. , 2021, Analytical chemistry.

[5]  Seok Joon Mun,et al.  Discontinuous Dewetting in a Degassed Mold for Fabrication of Homogeneous Polymeric Microparticles. , 2020, ACS applied materials & interfaces.

[6]  Tianzhun Wu,et al.  Ultrafast Microdroplet Generation and High-Density Microparticle Arraying Based on Biomimetic Nepenthes Peristome Surfaces. , 2020, ACS applied materials & interfaces.

[7]  Jia Zhou,et al.  Picoliter droplet array based on bioinspired microholes for in situ single-cell analysis , 2020, Microsystems & nanoengineering.

[8]  Qun Fang,et al.  Automated, flexible and versatile manipulation of nanoliter-to-picoliter droplets based on sequential operation droplet array technique , 2020 .

[9]  Pengfei Zhang,et al.  Customizing droplet contents and dynamic ranges via integrated programmable picodroplet assembler , 2019, Microsystems & Nanoengineering.

[10]  P. Levkin,et al.  Marrying chemistry with biology by combining on-chip solution-based combinatorial synthesis and cellular screening , 2019, Nature Communications.

[11]  Saeid Nahavandi,et al.  A self-sufficient micro-droplet generation system using highly porous elastomeric sponges: A versatile tool for conducting cellular assays , 2018, Sensors and Actuators B: Chemical.

[12]  P. Levkin,et al.  Droplet Microarrays: From Surface Patterning to High‐Throughput Applications , 2018, Advanced materials.

[13]  Min Yu,et al.  Droplet Array-Based 3D Coculture System for High-Throughput Tumor Angiogenesis Assay. , 2018, Analytical chemistry.

[14]  Mingjie Liu,et al.  Nature-inspired superwettability systems , 2017 .

[15]  J. Lammertyn,et al.  Single-Step Imprinting of Femtoliter Microwell Arrays Allows Digital Bioassays with Attomolar Limit of Detection. , 2017, ACS applied materials & interfaces.

[16]  Huiyu Low,et al.  Clarity™ digital PCR system: a novel platform for absolute quantification of nucleic acids , 2017, Analytical and Bioanalytical Chemistry.

[17]  G. Sui,et al.  High-Throughput Microfluidic Device for LAMP Analysis of Airborne Bacteria , 2016 .

[18]  Wenqian Feng,et al.  Single‐Step Fabrication of High‐Density Microdroplet Arrays of Low‐Surface‐Tension Liquids , 2016, Advanced materials.

[19]  Allon M. Klein,et al.  Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells , 2015, Cell.

[20]  Evan Z. Macosko,et al.  Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets , 2015, Cell.

[21]  Q. Fang,et al.  Swan probe: A nanoliter-scale and high-throughput sampling interface for coupling electrospray ionization mass spectrometry with microfluidic droplet array and multiwell plate. , 2014, Analytical chemistry.

[22]  A. I. Neto,et al.  Biomimetic Miniaturized Platform Able to Sustain Arrays of Liquid Droplets for High‐Throughput Combinatorial Tests , 2014 .

[23]  Weiqing Ren,et al.  Wetting transition on patterned surfaces: transition states and energy barriers. , 2014, Langmuir : the ACS journal of surfaces and colloids.

[24]  P. Levkin,et al.  Emerging Applications of Superhydrophilic‐Superhydrophobic Micropatterns , 2013, Advanced materials.

[25]  C. V. van Blitterswijk,et al.  Spheroid culture as a tool for creating 3D complex tissues. , 2013, Trends in biotechnology.

[26]  Charles N. Baroud,et al.  Droplet microfluidics driven by gradients of confinement , 2013, Proceedings of the National Academy of Sciences.

[27]  Pengyu Lv,et al.  Importance of hierarchical structures in wetting stability on submersed superhydrophobic surfaces. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[28]  Bing Sun,et al.  Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral load. , 2011, Journal of the American Chemical Society.

[29]  P. Levkin,et al.  A Facile Approach to Superhydrophilic–Superhydrophobic Patterns in Porous Polymer Films , 2011, Advanced materials.

[30]  K. Isselbacher,et al.  Isolation of circulating tumor cells using a microvortex-generating herringbone-chip , 2010, Proceedings of the National Academy of Sciences.

[31]  James J. Feng,et al.  Enhanced slip on a patterned substrate due to depinning of contact line , 2009 .

[32]  Andrew D Griffiths,et al.  Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. , 2009, Analytical chemistry.

[33]  Michael G. Roper,et al.  A fully integrated microfluidic genetic analysis system with sample-in–answer-out capability , 2006, Proceedings of the National Academy of Sciences.

[34]  Kazuhito Hashimoto,et al.  Effects of Surface Structure on the Hydrophobicity and Sliding Behavior of Water Droplets , 2002 .