Preparation and Maintenance of Organotypic Cultures for Multi‐Electrode Array Recordings

Preparation and Maintenance of Organotypic Cultures for Multi‐Electrode Array Recordings (Veronica C. Karpiak and Dietmar Plenz, National Institutes of Mental Health, NIH, Bethesda, Maryland). Recording from neuronal cultures with multi‐electrode arrays (MEAs) provides a powerful tool for studying neuronal activity with many neurons simultaneously in vitro. This unit describes the detailed steps necessary for growing organotypic cultures on MEAs and the typical neuronal activity that is obtained with this methodology.

[1]  U. Egert,et al.  A thin film microelectrode array for monitoring extracellular neuronal activity in vitro. , 1994, Biosensors & bioelectronics.

[2]  G. Gross,et al.  Multielectrode analysis of coordinated, multisite, rhythmic bursting in cultured CNS monolayer networks , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[3]  L. Stoppini,et al.  A new extracellular multirecording system for electrophysiological studies: application to hippocampal organotypic cultures , 1997, Journal of Neuroscience Methods.

[4]  G. Gross,et al.  Stimulation of monolayer networks in culture through thin-film indium-tin oxide recording electrodes , 1993, Journal of Neuroscience Methods.

[5]  N.F. de Rooij,et al.  Microelectrode arrays for electrophysiological monitoring of hippocampal organotypic slice cultures , 1997, IEEE Transactions on Biomedical Engineering.

[6]  L. Paninski,et al.  Information about movement direction obtained from synchronous activity of motor cortical neurons. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Plenz,et al.  Up and Down States in Striatal Medium Spiny Neurons Simultaneously Recorded with Spontaneous Activity in Fast-Spiking Interneurons Studied in Cortex–Striatum–Substantia Nigra Organotypic Cultures , 1998, The Journal of Neuroscience.

[8]  U. Egert,et al.  A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays. , 1998, Brain research. Brain research protocols.

[9]  D. Plenz,et al.  Morphological organization of the globus pallidus‐subthalamic nucleus system studied in organotypic cultures , 1998, The Journal of comparative neurology.

[10]  A. Aertsen,et al.  Dynamics of neuronal interactions in monkey cortex in relation to behavioural events , 1995, Nature.

[11]  D. Debanne,et al.  Organotypic slice cultures: a technique has come of age , 1997, Trends in Neurosciences.

[12]  Christopher R. Stambaugh,et al.  Simultaneous encoding of tactile information by three primate cortical areas , 1998, Nature Neuroscience.