Genetic, metabolomic and transcriptomic analyses of the de novo L-cysteine biosynthetic pathway in the enteric protozoan parasite Entamoeba histolytica

[1]  G. Schneider,et al.  Inhibitors of the Cysteine Synthase CysM with Antibacterial Potency against Dormant Mycobacterium tuberculosis. , 2016, Journal of Medicinal Chemistry.

[2]  T. Nozaki,et al.  Heterogeneity of the serine synthetic pathway in Entamoeba species. , 2016, Molecular and biochemical parasitology.

[3]  T. Nozaki,et al.  Entamoeba thiol-based redox metabolism: A potential target for drug development. , 2016, Molecular and biochemical parasitology.

[4]  S. Ōmura,et al.  Identification of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites , 2015, Front. Microbiol..

[5]  A. Mozzarelli,et al.  Moonlighting O-acetylserine sulfhydrylase: New functions for an old protein. , 2015, Biochimica et biophysica acta.

[6]  U. Singh,et al.  Dimethylated H3K27 Is a Repressive Epigenetic Histone Mark in the Protist Entamoeba histolytica and Is Significantly Enriched in Genes Silenced via the RNAi Pathway* , 2015, The Journal of Biological Chemistry.

[7]  T. Nozaki,et al.  Entamoeba mitosomes play an important role in encystation by association with cholesteryl sulfate synthesis , 2015, Proceedings of the National Academy of Sciences.

[8]  T. Nozaki,et al.  Phenotypic and transcriptional profiling in Entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance , 2015, Front. Microbiol..

[9]  P. Kao,et al.  Update on Diagnosis and Management , 2015 .

[10]  Haruo Watanabe,et al.  Mass Spectrometric Analysis of l-Cysteine Metabolism: Physiological Role and Fate of l-Cysteine in the Enteric Protozoan Parasite Entamoeba histolytica , 2014, mBio.

[11]  T. Nozaki,et al.  Metabolomic analysis of Entamoeba: applications and implications. , 2014, Current opinion in microbiology.

[12]  J. Tarábek,et al.  Novel Functions of an Iron-Sulfur Flavoprotein from Trichomonas vaginalis Hydrogenosomes , 2014, Antimicrobial Agents and Chemotherapy.

[13]  L. Romero,et al.  Cysteine and cysteine-related signaling pathways in Arabidopsis thaliana. , 2014, Molecular plant.

[14]  Pietro Cozzini,et al.  Isozyme-Specific Ligands for O-acetylserine sulfhydrylase, a Novel Antibiotic Target , 2013, PloS one.

[15]  T. Nozaki,et al.  Transcriptome Analysis of Encystation in Entamoeba invadens , 2013, PloS one.

[16]  M. Suematsu,et al.  Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica. , 2013, Biochimie.

[17]  C. Nowicki,et al.  Functional characterization of enzymes involved in cysteine biosynthesis and H(2)S production in Trypanosoma cruzi. , 2012, Molecular and biochemical parasitology.

[18]  T. Soga,et al.  Dramatic Increase in Glycerol Biosynthesis upon Oxidative Stress in the Anaerobic Protozoan Parasite Entamoeba histolytica , 2012, PLoS neglected tropical diseases.

[19]  Alejandro E Leroux,et al.  Low-molecular-mass antioxidants in parasites. , 2012, Antioxidants & redox signaling.

[20]  M. Suematsu,et al.  Metabolic Profiling of the Protozoan Parasite Entamoeba invadens Revealed Activation of Unpredicted Pathway during Encystation , 2012, PloS one.

[21]  T. Nozaki,et al.  A novel class of cysteine protease receptors that mediate lysosomal transport , 2012, Cellular microbiology.

[22]  N. Subbarao,et al.  Virtual Screening, Identification and In Vitro Testing of Novel Inhibitors of O-Acetyl-L-Serine Sulfhydrylase of Entamoeba histolytica , 2012, PloS one.

[23]  T. Nozaki,et al.  Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica , 2011, BMC Genomics.

[24]  N. Subbarao,et al.  Structural and Biochemical Studies of Serine Acetyltransferase Reveal Why the Parasite Entamoeba histolytica Cannot Form a Cysteine Synthase Complex* , 2011, The Journal of Biological Chemistry.

[25]  T. Nozaki,et al.  Conservation and function of Rab small GTPases in Entamoeba: annotation of E. invadens Rab and its use for the understanding of Entamoeba biology. , 2010, Experimental parasitology.

[26]  M. Suematsu,et al.  Metabolome Analysis Revealed Increase in S-Methylcysteine and Phosphatidylisopropanolamine Synthesis upon l-Cysteine Deprivation in the Anaerobic Protozoan Parasite Entamoeba histolytica* , 2010, The Journal of Biological Chemistry.

[27]  M. Suematsu,et al.  Two Atypical l-Cysteine-regulated NADPH-dependent Oxidoreductases Involved in Redox Maintenance, l-Cystine and Iron Reduction, and Metronidazole Activation in the Enteric Protozoan Entamoeba histolytica* , 2010, The Journal of Biological Chemistry.

[28]  A. Grossman,et al.  Binding of Cysteine Synthase to the STAS Domain of Sulfate Transporter and Its Regulatory Consequences* , 2010, The Journal of Biological Chemistry.

[29]  Neil Hall,et al.  New Assembly, Reannotation and Analysis of the Entamoeba histolytica Genome Reveal New Genomic Features and Protein Content Information , 2010, PLoS neglected tropical diseases.

[30]  Michele Sallese,et al.  The KDEL receptor: New functions for an old protein , 2009, FEBS letters.

[31]  Liliana Rojas,et al.  Reassessment of the epidemiology of amebiasis: state of the art. , 2009, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[32]  M. Tomita,et al.  Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles , 2009, Metabolomics.

[33]  G. H. Coombs,et al.  Two pathways for cysteine biosynthesis in Leishmania major. , 2009, The Biochemical journal.

[34]  Masaru Tomita,et al.  Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry. , 2009, Analytical chemistry.

[35]  R. Wade,et al.  A mechanistic model of the cysteine synthase complex. , 2009, Journal of molecular biology.

[36]  T. Nozaki,et al.  Isoform-dependent feedback regulation of serine O-acetyltransferase isoenzymes involved in L-cysteine biosynthesis of Entamoeba histolytica. , 2009, Molecular and biochemical parasitology.

[37]  S. Gourinath,et al.  Crystal structure of native O‐acetyl‐serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: Structural evidence for cysteine binding and lack of interactions with serine acetyl transferase , 2008, Proteins.

[38]  Alok Bhattacharya,et al.  Inhibitors of Escherichia coli serine acetyltransferase block proliferation of Entamoeba histolytica trophozoites. , 2008, International journal for parasitology.

[39]  T. Nozaki,et al.  Kinetic characterization of methionine γ‐lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis , 2008, The FEBS journal.

[40]  Masaru Tomita,et al.  Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. , 2008, Molecular bioSystems.

[41]  M. Salles,et al.  Invasive amebiasis: an update on diagnosis and management , 2007, Expert review of anti-infective therapy.

[42]  T. Nozaki,et al.  Current Therapeutics, Their Problems, and Sulfur-Containing-Amino-Acid Metabolism as a Novel Target against Infections by “Amitochondriate” Protozoan Parasites , 2007, Clinical Microbiology Reviews.

[43]  Masaru Tomita,et al.  MathDAMP: a package for differential analysis of metabolite profiles , 2006, BMC Bioinformatics.

[44]  S. Ravanel,et al.  Methionine catabolism in Arabidopsis cells is initiated by a γ-cleavage process and leads to S-methylcysteine and isoleucine syntheses , 2006, Proceedings of the National Academy of Sciences.

[45]  M. Tomita,et al.  Differential Metabolomics Reveals Ophthalmic Acid as an Oxidative Stress Biomarker Indicating Hepatic Glutathione Consumption* , 2006, Journal of Biological Chemistry.

[46]  M. Anbar,et al.  Transcriptional Silencing of Multiple Genes in Trophozoites of Entamoeba histolytica , 2006, PLoS pathogens.

[47]  R. Abagyan,et al.  XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. , 2006, Analytical chemistry.

[48]  T. Veziroglu,et al.  Role of Fe-hydrogenase in biological hydrogen production , 2006 .

[49]  T. Nozaki,et al.  Sulfur-containing amino acid metabolism in parasitic protozoa. , 2005, Advances in parasitology.

[50]  G. Garber,et al.  Treatment of Infections Caused by Metronidazole-Resistant Trichomonas vaginalis , 2004, Clinical Microbiology Reviews.

[51]  A. Sirko,et al.  Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects. , 2004, Journal of experimental botany.

[52]  H. Hesse,et al.  Molecular analysis and control of cysteine biosynthesis: integration of nitrogen and sulphur metabolism. , 2004, Journal of experimental botany.

[53]  T. Nozaki,et al.  Identification and characterization of two isoenzymes of methionine gamma-lyase from Entamoeba histolytica: a key enzyme of sulfur-amino acid degradation in an anaerobic parasitic protist that lacks forward and reverse trans-sulfuration pathways. , 2003, The Journal of biological chemistry.

[54]  M. Ghirardi,et al.  Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions. , 2003, European journal of biochemistry.

[55]  D. Mirelman,et al.  Transcriptional Silencing of an Amoebapore Gene in Entamoeba histolytica: Molecular Analysis and Effect on Pathogenicity , 2003, Eukaryotic Cell.

[56]  C. Clark,et al.  Methods for Cultivation of Luminal Parasitic Protists of Clinical Importance , 2002, Clinical Microbiology Reviews.

[57]  Masaru Tomita,et al.  Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry. , 2002, Analytical chemistry.

[58]  T. Soga,et al.  Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry. , 2000, Analytical chemistry.

[59]  T. Nozaki,et al.  Characterization of the gene encoding serine acetyltransferase, a regulated enzyme of cysteine biosynthesis from the protist parasites Entamoeba histolytica and Entamoeba dispar. Regulation and possible function of the cysteine biosynthetic pathway in Entamoeba. , 1999, The Journal of biological chemistry.

[60]  E. Tannich,et al.  Metronidazole Resistance in the Protozoan Parasite Entamoeba histolytica Is Associated with Increased Expression of Iron-containing Superoxide Dismutase and Peroxiredoxin and Decreased Expression of Ferredoxin 1 and Flavin Reductase* , 1999, The Journal of Biological Chemistry.

[61]  J. Sekiya,et al.  Rapid purification and characterization of cystine lyase b from broccoli inflorescence , 1999 .

[62]  Ben Shneiderman,et al.  Applications and implications , 1999 .

[63]  T. Nozaki,et al.  Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasite Entamoeba histolytica. , 1998, Molecular and biochemical parasitology.

[64]  Markus Voelter,et al.  State of the Art , 1997, Pediatric Research.

[65]  G. Stoewsand,et al.  S-Methylcysteine sulfoxide in Brassica vegetables and formation of methyl methanethiosulfinate from Brussels sprouts , 1992 .

[66]  G. Snider.  Protease‐antiprotease imbalance in the pathogensis of emphysema and chronic bronchial injury: A potential target for drug development , 1987 .

[67]  L S Diamond,et al.  A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. , 1978, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[68]  T. Mae,et al.  Metabolism of S-methylcysteine and its sulfoxide in Chinese cabbage, Brassica pekinensis Rupr. , 1971 .