Air sampling for fungi in indoor environments

Abstract Mould growth in buildings is a major health issue, but most investigations of the indoor air spora still employ culture-based methods. These are inadequate for assessing exposure, since culturable organisms comprise a small fraction of the total of potentially allergenic/toxigenic units in air. For epidemiological studies, measurement of airborne fungal biomass over extended periods may be more relevant than total counts. Whilst (1 → 3)-β- d -glucan has been used to assess airborne biomass, ergosterol may be the best indicator of exposure. For case studies, patients' serum has been used to detect specific spores on sampler slides, and both highly specific and less specific antisera could be used either via fluorescent antibody technique or enzyme-linked immunosorbent assays. In the future, solid-phase polymerase chain reaction (PCR) may be used to detect pathogens or other well-characterized potentially harmful species, and in at least some groups mycotoxin/secondary metabolite/volatile profiles may be used in identification.

[1]  B. Brunekreef,et al.  Presence of Viable Mould Propagules in the Indoor Air of Houses , 1990, Toxicology and industrial health.

[2]  E. M. McCabe,et al.  Allergenic and toxigenic micro-organisms in houses. , 1991, Society for Applied Bacteriology symposium series.

[3]  J. Young.  Microwave-assisted extraction of the fungal metabolite ergosterol and total fatty acids , 1995 .

[4]  J. Jaakkola,et al.  Home dampness and molds as determinants of respiratory symptoms and asthma in pre-school children. , 1993, Journal of exposure analysis and environmental epidemiology.

[5]  Shigenori Tanaka,et al.  Airborne Beta-1,3-Glucan May Be Related to Symptoms in Sick Buildings , 1992 .

[6]  J. Miller,et al.  The use of ergosterol to measure exposure to fungal propagules in indoor air. , 1997, American Industrial Hygiene Association journal.

[7]  P. Thorne,et al.  Bioaerosol Concentrations in Noncomplaint, Complaint, and Intervention Homes in the Midwest , 1995 .

[8]  P. Malmberg,et al.  The nuclepore filter method: A technique for enumeration of viable and nonviable airborne microorganisms , 1986 .

[9]  S. Gravesen,et al.  Health implications of fungi in indoor environments. , 1994 .

[10]  Monoclonal antibodies for the detection of spoilage fungi , 1993 .

[11]  C. J. Mirocha,et al.  Chitin as a measure of fungal growth in soured corn and soybean seed , 1977 .

[12]  P. Kozak,et al.  Currently available methods for home mold surveys. II. Examples of problem homes surveyed. , 1980, Annals of allergy.

[13]  R. Burnett,et al.  Respiratory health effects of home dampness and molds among Canadian children. , 1991, American journal of epidemiology.

[14]  R. Burnett,et al.  Adverse health effects among adults exposed to home dampness and molds. , 1991, The American review of respiratory disease.

[15]  J. Miller,et al.  Fungi and fungal products in some Canadian houses , 1988 .

[16]  A A ANDERSEN,et al.  NEW SAMPLER FOR THE COLLECTION, SIZING, AND ENUMERATION OF VIABLE AIRBORNE PARTICLES, , 1958, Journal of bacteriology.

[17]  B. Brunekreef,et al.  Enumeration and identification of airborne viable mould propagules in houses , 1990, Allergy.

[18]  B. Flannigan.  Indoor Microbiological Pollutants — Sources, Species, Characterisation and Evaluation , 1992 .

[19]  Personal spore sampling and indirect immunofluorescent test for exploration of hypersensitivity pneumonitis due to mould spores , 1991, Allergy.

[20]  R. Rylander,et al.  Pulmonary inflammation induced by repeated inhalations of beta(1,3)-D-glucan and endotoxin. , 1994, International journal of experimental pathology.

[21]  T. Novitsky,et al.  Sensitivity of Limulus amebocyte lysate (LAL) to LAL-reactive glucans , 1991, Journal of clinical microbiology.

[22]  Peder Wolkoff,et al.  Chemical, microbiological, health and comfort aspects of indoor air quality : state of the art in SBS , 1992 .

[23]  J. Kay,et al.  Isolation and characterization of beta-glucan receptors on human mononuclear phagocytes , 1991, The Journal of experimental medicine.

[24]  E. M. McCabe,et al.  Quantification of airborne moulds in the homes of children with and without wheeze. , 1990, Thorax.

[25]  Robert A. Samson,et al.  Health Implications of Fungi in Indoor Environments , 1994 .

[26]  K L Josephson,et al.  Polymerase chain reaction detection of nonviable bacterial pathogens , 1993, Applied and environmental microbiology.

[27]  B. Kendrick,et al.  A year-round comparison of fungal spores in indoor and outdoor air , 1995 .

[28]  J. Frisvad,et al.  Chemosystematics of Penicillium based on profiles of volatile metabolites , 1995 .

[29]  P. Malmberg,et al.  Collection of airborne micro-organisms on Nuclepore filters, estimation and analysis--CAMNEA method. , 1986, The Journal of applied bacteriology.

[30]  M. Swift.  The estimation of mycelial biomass by determination of the hexosamine content of wood tissue decayed by fungi , 1973 .

[31]  A. Fradkin.  Sampling of Microbiological Contaminants in Indoor Air , 1987 .

[32]  G. Toranzos,et al.  Use of solid-phase PCR for enhanced detection of airborne microorganisms , 1994, Applied and environmental microbiology.

[33]  A. Fox,et al.  Quantification of Muramic Acid, a Marker for Bacterial Peptidoglycan, in Dust Collected from Hospital and Home Air‐Conditioning Filters using Gas Chromatography — Mass Spectrometry , 1994 .

[34]  J D Spengler,et al.  Home dampness and respiratory morbidity in children. , 1989, The American review of respiratory disease.

[35]  J. Frisvad,et al.  Production of volatiles and presence of mycotoxins in conidia of common indoor penicillia and aspergilli. , 1994 .

[36]  L. Stetzenbach,et al.  Monitoring airborne fungal spores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling , 1993, Applied and environmental microbiology.

[37]  B. Flannigan,et al.  Mould in buildings: the air spora of domestic dwellings , 1988 .

[38]  Y. Sasamoto,et al.  Rapid method for preparing a beta-glucan-specific sensitive fraction from Limulus (Tachypleus tridentatus) amebocyte lysate. , 1991, Journal of chromatography.

[39]  A. Verhoeff,et al.  Health implications of fungi in indoor environments - an overview. , 1994 .

[40]  A. Fox,et al.  Monitoring of Bacterial Sugars and Hydroxy Fatty Acids in Dust from Air Conditioners by Gas Chromatography-Mass Spectrometry , 1993, Applied and environmental microbiology.

[41]  Klaus Willeke,et al.  Performance of bioaerosol samplers: collection characteristics and sampler design considerations , 1992 .

[42]  A. F. Bravery,et al.  The moisture requirements of moulds isolated from domestic dwellings , 1989 .