Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices.

We present a microtiter plate-sized standalone chip holder for precise control of physiological conditions inside closed microfluidic cell culture systems, made from gas-impermeable materials. Specifically, we demonstrate the suitability of the holder to support cell growth in a glass chip, to allow time-lapse imaging of live cells and the creation of a hypoxic environment, all relevant for applications in regenerative medicine research.

[1]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[2]  D. Eddington,et al.  A microfabricated platform for establishing oxygen gradients in 3-D constructs , 2013, Biomedical microdevices.

[3]  Abdallah S Daar,et al.  A proposed definition of regenerative medicine , 2007, Journal of tissue engineering and regenerative medicine.

[4]  Shuichi Takayama,et al.  Quantitative measurement and control of oxygen levels in microfluidic poly(dimethylsiloxane) bioreactors during cell culture , 2007, Biomedical microdevices.

[5]  Chien-Chung Peng,et al.  A microfluidic cell culture array with various oxygen tensions. , 2013, Lab on a chip.

[6]  David Beebe,et al.  Engineers are from PDMS-land, Biologists are from Polystyrenia. , 2012, Lab on a chip.

[7]  Pamela Habibovic,et al.  Regeneration-on-a-chip? The perspectives on use of microfluidics in regenerative medicine. , 2013, Lab on a chip.

[8]  Nicolas Szita,et al.  Oxygen Transfer Characteristics of Miniaturized Bioreactor Systems , 2013, Biotechnology and bioengineering.

[9]  Albert van den Berg.  Quantitative biosciences from nano to macro Indexed in MEDLINE ! , 2012 .

[10]  Lewis D. Griffin,et al.  Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development , 2012, PloS one.

[11]  Jun Liu,et al.  Monitoring nutrient transport in tissue‐engineered grafts , 2015, Journal of tissue engineering and regenerative medicine.

[12]  B. Cui,et al.  A microfluidic positioning chamber for long‐term live‐cell imaging , 2011, Microscopy research and technique.

[13]  J. Tramper,et al.  Oxygen gradients in tissue‐engineered Pegt/Pbt cartilaginous constructs: Measurement and modeling , 2004, Biotechnology and bioengineering.