Reactivation of human placental 17β, 20α-hydroxysteroid dehydrogenase: Affirmation of affinity labeling principles

[1]  R. Strickler,et al.  Inactivation of human placental 17 beta,20 alpha-hydroxysteroid dehydrogenase by 16-methylene estrone, an affinity alkylator enzymatically generated from 16-methylene estradiol-17 beta. , 1983, The Journal of biological chemistry.

[2]  R. Strickler,et al.  Human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. Studies with 6 beta-bromoacetoxyprogesterone. , 1983, The Journal of biological chemistry.

[3]  R. Strickler,et al.  Inactivation of human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase with active site-directed 17 beta-propynyl-substituted progestin analogs. , 1982, The Journal of biological chemistry.

[4]  J. C. Warren,et al.  Isolation of histidyl peptides of the steroid-binding site of human placental estradiol 17β-dehydrogenase , 1982, Steroids.

[5]  R. Strickler,et al.  Affinity labeling of human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase with 5'-[p-(fluorosulfonyl)benzoyl]adenosine. , 1981, Biochemistry.

[6]  R. Strickler,et al.  Human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. Two activities at a single enzyme active site. , 1981, The Journal of biological chemistry.

[7]  R. Strickler,et al.  Estradiol 17β-dehydrogenase and 20α-hydroxysteroid dehydrogenase from human placental cytosol: one enzyme with two activities? , 1980, Steroids.

[8]  F. Sweet,et al.  Bifunctional enzyme activity at the same active site: study of 3 alpha and 20 beta activity by affinity alkylation of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids. , 1980, Biochemistry.

[9]  A. Crastes,et al.  Some new developments in the knowledge of human placental estradiol-17beta dehydrogenase. , 1977, Journal of steroid biochemistry.

[10]  J. B. Dence,et al.  Crystallization of human placental estradiol 17beta-dehydrogenase. A new method for crystallizing labile enzymes. , 1976, The Journal of biological chemistry.

[11]  F. Sweet Reactivation studies of an affinity labeled steroid oxido-reductase I. Inactivation of 20β-hydroxysteroid dehydrogenase with 6β-bromoacetoxyprogesterone vs 6β-bromoprogesterone , 1976, Steroids.

[12]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[13]  E. Groman,et al.  Catalytic competence, a new criterion for affinity labeling. Demonstration of the reversible enzymatic interconversion of estrone and estradiol-17 beta covalently bound to human placental estradiol-17 beta dehydrogenase. , 1975, The Journal of biological chemistry.

[14]  J. C. Warren,et al.  Synthesis and use of affinity labeling steroids for analysis of macromolecular steroid-binding sites. , 1975, Methods in enzymology.

[15]  J. Nicolas,et al.  (II) Enhancement of the catalytic activity of the human placental 17 β‐oestradiol dehydrogenase by carboxymethylation of an histidyl residue of the active site , 1973, FEBS letters.

[16]  J. C. Warren,et al.  Purification of estradiol-17 beta dehydrogenase from human placenta by affinity chromatography. , 1973, Steroids.