Effect of titanium-prepared platelet-rich fibrin treatment on the angiogenic biomarkers in gingival crevicular fluid in infrabony defects of patients with chronic periodontitis: A randomized controlled clinical trial

Aim: The aim of this double-blinded, randomized, controlled clinical study was to investigate the effect of titanium-prepared platelet-rich fibrin (T-PRF) treatment on the angiogenic biomarkers in gingival crevicular fluid (GCF) in infrabony defects of patients with chronic periodontitis. Materials and Methods: Twenty five systemically healthy participants who complied with inclusion criteria with periodontal infrabony defects were recruited. In each patient, the infrabony defect of one side of arch was designated as control group (allograft), whereas the infrabony defect on the contralateral side of same arch was designated as test group (allograft + T-PRF). The therapy methods (test or control) were randomly decided. GCF samples were collected at baseline (presurgery) and then the 3rd, 7th, 14th, and 30th days after surgery. Platelet-derived growth factor (PDGF)-BB, vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2, anjiogenin (ANG), angiostatin (ANT) in the GCF samples were measured using human enzyme-linked immunosorbent assay kits. Results: In both groups, total amounts of PDGF-BB, VEGF-A, FGF-2, ANG, and ANT peaked in the GCF samples obtained at the early postoperative day (day 3) and decreased over time in the samples obtained at the 7th, 14th, and 30th days postsurgery. There were no significant differences between groups for the total amounts of PDGF-BB, VEGF-A, FGF-2, ANG, and ANT at all evaluation periods. Conclusion: Application of T-PRF combined with allograft in infrabony defects of patients with chronic periodontitis had no significant effects on angiogenic biomarkers in GCF.

[1]  R. Miron,et al.  Platelet Rich Fibrin: A Second‐Generation Platelet Concentrate , 2017 .

[2]  C. Martínez,et al.  The influence of platelet-derived products on angiogenesis and tissue repair: a concise update , 2015, Front. Physiol..

[3]  Zafer Küçükodacı,et al.  A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin , 2014, BioMed research international.

[4]  R. Serra,et al.  Application of platelet‐rich gel to enhance healing of transmetatarsal amputations in diabetic dysvascular patients , 2013, International wound journal.

[5]  Zafer Küçükodacı,et al.  In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate. , 2013, The British journal of oral & maxillofacial surgery.

[6]  J. Bernard,et al.  Clinical and Histological Evaluation of Postextraction Platelet-rich Fibrin Socket Filling: A Prospective Randomized Controlled Study , 2012, Implant dentistry.

[7]  V. Bharti,et al.  Evaluation of efficacy of autologous platelet-rich fibrin with demineralized-freeze dried bone allograft in the treatment of periodontal intrabony defects , 2013, Journal of Indian Society of Periodontology.

[8]  Donald R. Hoaglin,et al.  Prevention of Localized Osteitis in Mandibular Third-Molar Sites Using Platelet-Rich Fibrin , 2013, International journal of dentistry.

[9]  Shuko Suzuki,et al.  Gelatin gel as a carrier of platelet-derived growth factors , 2013, Journal of biomaterials applications.

[10]  I. Asahina,et al.  Evaluation of sinus floor augmentation with simultaneous implant placement using platelet-rich fibrin as sole grafting material. , 2013, The International journal of oral & maxillofacial implants.

[11]  G. Atilla,et al.  Platelet‐Rich Fibrin in the Treatment of Bilateral Gingival Recessions , 2012 .

[12]  I. Milinkovic,et al.  Platelet-rich fibrin and bovine porous bone mineral vs. platelet-rich fibrin in the treatment of intrabony periodontal defects. , 2012, Journal of Periodontal Research.

[13]  K. Lu,et al.  Platelet-rich fibrin increases cell attachment, proliferation and collagen-related protein expression of human osteoblasts. , 2012, Australian dental journal.

[14]  D. M. Dohan Ehrenfest,et al.  Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). , 2012, Current pharmaceutical biotechnology.

[15]  D. M. Dohan Ehrenfest,et al.  The use of leukocyte- and platelet-rich fibrin during immediate postextractive implantation and loading for the esthetic replacement of a fractured maxillary central incisor. , 2012, The Journal of oral implantology.

[16]  Kavitarani Rudagi,et al.  One-step apexification in immature tooth using grey mineral trioxide aggregate as an apical barrier and autologus platelet rich fibrin membrane as an internal matrix , 2012, Journal of conservative dentistry : JCD.

[17]  S. Jankovic,et al.  Use of platelet-rich fibrin membrane following treatment of gingival recession: a randomized clinical trial. , 2012, The International journal of periodontics & restorative dentistry.

[18]  A. Pradeep,et al.  Autologous platelet-rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. , 2011, Journal of periodontology.

[19]  P. Boffano,et al.  Platelet-Rich Fibrin and Piezoelectric Surgery: A Safe Technique for the Prevention of Periodontal Complications in Third Molar Surgery , 2011, The Journal of craniofacial surgery.

[20]  L. Stephen,et al.  Alveolar Ridge Preservation Using Leukocyte and Platelet-Rich Fibrin: A Report of a Case , 2011, Case reports in dentistry.

[21]  G. Sammartino,et al.  Simultaneous Sinus-Lift and Implantation Using Microthreaded Implants and Leukocyte- and Platelet-Rich Fibrin as Sole Grafting Material: A Six-Year Experience , 2011, Implant dentistry.

[22]  K. Nagy,et al.  Treatment of class III multiple gingival recessions: a randomized-clinical trial. , 2010, Journal of clinical periodontology.

[23]  D. M. Dohan Ehrenfest,et al.  Sinus floor augmentation with simultaneous implant placement using Choukroun's platelet-rich fibrin as the sole grafting material: a radiologic and histologic study at 6 months. , 2009, Journal of periodontology.

[24]  Ye Lin,et al.  A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. , 2009, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[25]  G. Odin,et al.  In vitro effects of Choukroun's PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures. , 2009, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[26]  I. Springer,et al.  Platelet-rich plasma and platelet-rich fibrin in human cell culture. , 2009, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[27]  C. Su,et al.  In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. , 2009, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[28]  R. Flaumenhaft,et al.  Platelet alpha-granules: basic biology and clinical correlates. , 2009, Blood reviews.

[29]  T. Albrektsson,et al.  Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). , 2009, Trends in biotechnology.

[30]  D. M. Dohan Ehrenfest,et al.  Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies , 2009, Growth factors.

[31]  D. M. Dohan Ehrenfest,et al.  Re: "Clinical evaluation of a modified coronally advanced flap alone or in combination with a platelet-rich fibrin membrane for the treatment of adjacent multiple gingival recessions: a 6-month study". , 2009, Journal of periodontology.

[32]  Olivera Stojadinovic,et al.  PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing , 2008, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[33]  P. Nurden,et al.  Platelets and wound healing. , 2008, Frontiers in bioscience : a journal and virtual library.

[34]  S. O'connell,et al.  Safety issues associated with platelet-rich fibrin method. , 2007, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[35]  T. Bielecki,et al.  Why the platelet-rich gel has antimicrobial activity? , 2007, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[36]  A. Kantarcı,et al.  Platelet-Rich Plasma: Growth Factors and Pro- and Anti-Inflammatory , 2007 .

[37]  B. Eppley,et al.  Platelet-Rich Plasma: A Review of Biology and Applications in Plastic Surgery , 2006, Plastic and reconstructive surgery.

[38]  P. Koolwijk,et al.  Fibrin structure and wound healing , 2006, Journal of thrombosis and haemostasis : JTH.

[39]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[40]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[41]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates? , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[42]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[43]  S. Hayakawa,et al.  Platelet adhesion on titanium oxide gels: effect of surface oxidation. , 2004, Biomaterials.

[44]  Nathan E. Carlson,et al.  Platelet-rich plasma: clinical applications in dentistry. , 2002, Journal of the American Dental Association.

[45]  R. Marx,et al.  Platelet-rich plasma: Growth factor enhancement for bone grafts. , 1998, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.