No evidence of a correlation between BCL10 expression and API2‐MALT1 gene rearrangement in ocular adnexal MALT lymphoma

In the present study, 62 cases of ocular adnexal lymphoproliferative disorders were reviewed clinicopathologically. Of them, 51 were extranodal marginal zone B‐cell lymphoma (MALT lymphoma), five were diffuse large B‐cell lymphoma (DLBCL), one was peripheral T‐cell lymphoma, one was NK/T cell lymphoma, nasal type, and four were reactive lymphoid hyperplasia. These lymphoma cases showed a favorable clinical course and localized disease, except for the case of NK/T cell lymphoma, although 19 cases (32.8%) had a recurrence of disease. To clarify the correlation between BCL10 protein expression and API2–MALT1 gene rearrangement, the 51 cases of MALT lymphoma and 5 cases of DLBCL were analyzed by immunohistochemical and RT‐PCR methods. Nuclear BCL10 expression was identified in 58% of MALT lymphoma cases, but not in any DLBCL cases. There was no evidence of a correlation between aberrant nuclear BCL10 expression and the clinical parameters examined in the present study. API2–MALT1 transcription was not demonstrated in either the MALT lymphoma cases or the DLBCL cases studied using a multiplex one‐tube reverse transcriptase‐PCR method. These findings indicate that the nuclear expression of BCL10 is unlikely to correlate with the API2‐MALT1 fusion gene in ocular adnexal MALT lymphoma.

[1]  W. Lam,et al.  Primary cutaneous marginal zone B-cell lymphoma , 2006 .

[2]  Sindy Hu,et al.  Primary Cutaneous Marginal Zone B-Cell Lymphoma: A Molecular and Clinicopathologic Study of 24 Asian Cases , 2003, The American journal of surgical pathology.

[3]  K. Ohshima,et al.  API2-MALT1 fusion defines a distinctive clinicopathologic subtype in pulmonary extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue. , 2003, American Journal of Pathology.

[4]  G. Ott,et al.  T(14;18)(q32;q21) involving IGH and MALT1 is a frequent chromosomal aberration in MALT lymphoma. , 2003, Blood.

[5]  A. Sánchez-Aguilera,et al.  Cell cycle deregulation in B-cell lymphomas. , 2003, Blood.

[6]  K. Ohshima,et al.  Clinicopathological features of gastric B‐cell lymphoma: A series of 317 cases , 2002, Pathology international.

[7]  I. Bearzi,et al.  A clinicopathological study of 152 surgically treated primary gastric lymphomas with survival analysis of 109 high grade tumours. , 2002, Journal of clinical pathology.

[8]  D. Hossfeld.  E.S. Jaffe, N.L. Harris, H. Stein, J.W. Vardiman (eds). World Health Organization Classification of Tumours: Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues , 2002 .

[9]  C. de Wolf‐Peeters,et al.  BCL10 mutation does not represent an important pathogenic mechanism in gastric MALT-type lymphoma, and the presence of the API2-MLT fusion is associated with aberrant nuclear BCL10 expression. , 2002, Blood.

[10]  M. Du,et al.  Mucosa-associated lymphoid tissue lymphoma , 2002, Current opinion in hematology.

[11]  T. Molina,et al.  T(11;18) is a marker for all stage gastric MALT lymphomas that will not respond to H. pylori eradication. , 2001, Gastroenterology.

[12]  米積 昌克.  Detection of API2-MALT1 chimaeric gene in extranodal and nodal marginal zone B cell lymphoma by reverse transcription-polymerase chain reaction (PCR) and genomic long and accurate PCR analyses , 2002 .

[13]  F. Hosoda,et al.  Detection of t(11;18) in MALT-Type Lymphoma With Dual-Color Fluorescence In Situ Hybridization and Reverse Transcriptase–Polymerase Chain Reaction Analysis , 2001 .

[14]  M. Seto,et al.  Detection of AP12‐MALT1 chimaeric gene in extranodal and nodal marginal zone B‐cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses , 2001, British journal of haematology.

[15]  F. Hosoda,et al.  Detection of t(I 1; 18) in MALT-type lymphoma with dual-color fluorescence in situ hybridization and reverse transcriptase-polymerase chain reaction analysis. , 2001, Diagnostic molecular pathology : the American journal of surgical pathology, part B.

[16]  L. Medeiros,et al.  Marginal-zone B-cell lymphoma of extranodal mucosa-associated lymphoid tissue type: molecular genetics provides new insights into pathogenesis. , 2001, Advances in anatomic pathology.

[17]  R. Hamoudi,et al.  T(11;18)(q21;q21) is associated with advanced mucosa-associated lymphoid tissue lymphoma that expresses nuclear BCL10. , 2001, Blood.

[18]  T. Yoshino,et al.  Clinical, Histopathological, and Immunogenetic Analysis of Ocular Adnexal Lymphoproliferative Disorders: Characterization of MALT Lymphoma and Reactive Lymphoid Hyperplasia , 2001, Modern Pathology.

[19]  M. Seto,et al.  Bcl10 and MALT1, Independent Targets of Chromosomal Translocation in MALT Lymphoma, Cooperate in a Novel NF-κB Signaling Pathway* , 2001, The Journal of Biological Chemistry.

[20]  M. Seto,et al.  API2-MALT1 fusion transcripts involved in mucosa-associated lymphoid tissue lymphoma: multiplex RT-PCR detection using formalin-fixed paraffin-embedded specimens. , 2001, The American journal of pathology.

[21]  M. Dyer,et al.  BCL10 expression in normal and neoplastic lymphoid tissue. Nuclear localization in MALT lymphoma. , 2000, The American journal of pathology.

[22]  E. Koonin,et al.  Identification of paracaspases and metacaspases: two ancient families of caspase-like proteins, one of which plays a key role in MALT lymphoma. , 2000, Molecular cell.

[23]  A. Stoffel,et al.  The API2/MALT1 Fusion Product May Lead to Germinal Center B Cell Lymphomas by Suppression of Apoptosis , 2000, Human Heredity.

[24]  P. Gaulard,et al.  Detection of t(11;18)(q21;q21) by interphase fluorescence in situ hybridization using API2 and MLT specific probes. , 2000, Blood.

[25]  M. Dyer,et al.  BCL10 gene mutation in lymphoma. , 2000, Blood.

[26]  C. James,et al.  Incidence and subtype specificity of API2-MALT1 fusion translocations in extranodal, nodal, and splenic marginal zone lymphomas. , 2000, The American journal of pathology.

[27]  P. Marynen,et al.  The product of the t(11;18), an API2-MLT fusion, marks nearly half of gastric MALT type lymphomas without large cell proliferation. , 2000, The American journal of pathology.

[28]  M. Seto,et al.  API2-MALT1 chimeric transcripts involved in mucosa-associated lymphoid tissue type lymphoma predict heterogeneous products. , 2000, The American journal of pathology.

[29]  T. Yoshino,et al.  Clinicopathological features of gastric mucosa associated lymphoid tissue (MALT) lymphomas: high grade transformation and comparison with diffuse large B cell lymphomas without MALT lymphoma features , 2000, Journal of clinical pathology.

[30]  A. Rosenwald,et al.  Heterogeneity of the API2-MALT1 gene rearrangement in MALT-type lymphoma , 2000, Leukemia.

[31]  A. Rosenwald,et al.  Exclusive detection of the t(11;18)(q21;q21) in extranodal marginal zone B cell lymphomas (MZBL) of MALT type in contrast to other MZBL and extranodal large B cell lymphomas. , 1999, The American journal of pathology.

[32]  P. Marynen,et al.  The apoptosis inhibitor gene API2 and a novel 18q gene, MLT, are recurrently rearranged in the t(11;18)(q21;q21) associated with mucosa-associated lymphoid tissue lymphomas. , 1999, Blood.

[33]  V. Curtin,et al.  Ocular-adnexal lymphoid tumors: a clinicopathologic and molecular genetic study of 77 patients. , 1999, Ophthalmic plastic and reconstructive surgery.

[34]  J. Arends,et al.  Gastric low-grade MALT lymphoma, high-grade MALT lymphoma and diffuse large B cell lymphoma show different frequencies of trisomy , 1999, Leukemia.

[35]  Bernd Hinzmann,et al.  Inactivating mutations and overexpression of BCL10, a caspase recruitment domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32) , 1999, Nature Genetics.

[36]  M. Dyer,et al.  Bcl10 Is Involved in t(1;14)(p22;q32) of MALT B Cell Lymphoma and Mutated in Multiple Tumor Types , 1999, Cell.

[37]  W. R. Lee,et al.  Lymphoproliferative lesions of the ocular adnexa. Analysis of 112 cases. , 1998, Ophthalmology.

[38]  M. Dyer,et al.  Bcl 10 Is Involved in t ( 1 ; 14 ) ( p 22 ; q 32 ) of MALT B Cell Lymphoma and Mutated in Multiple Tumor , 1998 .

[39]  T. Greiner,et al.  t(11;18)(q21;q21) is the most common translocation in MALT lymphomas. , 1997, Annals of oncology : official journal of the European Society for Medical Oncology.

[40]  A. Rosenwald,et al.  The t(11;18)(q21;q21) chromosome translocation is a frequent and specific aberration in low-grade but not high-grade malignant non-Hodgkin's lymphomas of the mucosa-associated lymphoid tissue (MALT-) type. , 1997, Cancer research.

[41]  P. Isaacson,et al.  Genetic evidence for a clonal link between low and high‐grade components in gastric MALT B‐cell lymphoma , 1997, Histopathology.

[42]  P. Isaacson,et al.  c‐myc GENE ABNORMALITIES IN MUCOSA‐ASSOCIATED LYMPHOID TISSUE (MALT) LYMPHOMAS , 1997, The Journal of pathology.

[43]  M. Tsuneyoshi,et al.  Helicobacter pylori and primary gastric lymphoma , 1997, Cancer.

[44]  M. Du,et al.  The accumulation of p53 abnormalities is associated with progression of mucosa-associated lymphoid tissue lymphoma. , 1995, Blood.

[45]  N. Harris,et al.  Ocular adnexal lymphoma. A clinicopathologic study with identification of lymphomas of mucosa-associated lymphoid tissue type. , 1995, Ophthalmology.

[46]  C. Montalbán,et al.  Low grade gastric B‐cell MALT lymphoma progressing into high grade lymphoma. Clonal identity of the two stages of the tumour, unusual bone involvement and leukaemic dissemination , 1995, Histopathology.

[47]  D. Knowles,et al.  Lymphoid hyperplasia and malignant lymphoma occurring in the ocular adnexa (orbit, conjunctiva, and eyelids): a prospective multiparametric analysis of 108 cases during 1977 to 1987. , 1990, Human pathology.

[48]  J. Chan,et al.  Relationship between high-grade lymphoma and low-grade B-cell mucosa-associated lymphoid tissue lymphoma (MALToma) of the stomach. , 1990, The American journal of pathology.

[49]  C. Bloomfield,et al.  Four new recurring translocations in non-Hodgkin lymphoma. , 1989, Blood.

[50]  M Tubiana,et al.  Report of the Committee on Hodgkin's Disease Staging Classification. , 1971, Cancer research.