Atypical reaction of 'essential' sulfhydryl groups of malic enzyme with 2-nitro-5-thiocyanobenzoate and 2,4-dinitrophenylthiocyanate.

[1]  F. Dallocchio,et al.  Evidence for multiple pairs of vicinal thiols in some proteins. , 1981, Journal of Biological Chemistry.

[2]  C. Degani,et al.  Further evidence for nonsymmetric subunit association and intersubunit cooperativity in creatine kinase. Subunit-selective modifications by 2,4-dinitrophenylthiocyanate. , 1980, Journal of Biological Chemistry.

[3]  R. Y. Hsu,et al.  Equilibrium substrate binding studies of the malic enzyme of pigeon liver. Equivalence of nucleotide sites and anticooperativity associated with the binding of L-malate to the enzyme-manganese(II)-reduced nicotinamide adenine dinucleotide phosphate ternary complex. , 1980, Biochemistry.

[4]  C. Degani,et al.  Subunit-selective chemical modifications of creatine kinase. Evidence for asymmetrical association of the subunits. , 1979, Biochemistry.

[5]  K. Dalziel,et al.  Purification of malic enzyme from bovine heart mitochondria by affinity chromatography. , 1979, Analytical biochemistry.

[6]  R. Y. Hsu,et al.  Mechanism of pigeon liver malic enzyme. Reactivity of class II sulfhydryl groups as a conformational probe for the "half-of-the-sites" reactivity of the enzyme with bromopyruvate. , 1978, Biochemistry.

[7]  G. Chang,et al.  Mechanism of pigeon liver malic enzyme: kinetics, specificity, and half-site stoichiometry of the alkylation of a cysteinyl residue by the substrate-inhibitor bromopyruvate. , 1977, Biochemistry.

[8]  N. C. Price.  Alternative products in the reaction of 2-nitro-5-thiocyanatobenzoic acid with thiol groups. , 1976, The Biochemical journal.

[9]  K. Yeang,et al.  Purification of malic enzyme by affinity chromatography on immobilized N6-(6-aminohexyl)-adenosine 2',5'-bisphosphate. , 1976, Analytical biochemistry.

[10]  R. Y. Hsu,et al.  Mechanism of pigeon liver malic enzyme. Modification of sulfhydryl groups by 5,5'-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide. , 1974, The Journal of biological chemistry.

[11]  A. Patchornik,et al.  Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues. , 1974, Biochemistry.

[12]  A. Patchornik,et al.  Selective cyanylation of sulfhydryl groups. II. Synthesis of 2-nitro-5-thiocyanatobenzoic acid , 1971 .

[13]  R. Chalkley,et al.  High resolution acrylamide gel electrophoresis of histones. , 1969, Archives of biochemistry and biophysics.

[14]  R. Y. Hsu,et al.  Pigeon liver malic enzyme. II. Isolation, crystallization, and some properties. , 1967, The Journal of biological chemistry.

[15]  T. A. Mahowald.  THE AMINO ACID SEQUENCE AROUND THE "REACTIVE" SULFHYDRYL GROUPS IN ADENOSINE TRIPHOSPHOCREATINE PHOSPHOTRANSFERASE. , 1965, Biochemistry.

[16]  M. Sokolovsky,et al.  Nonenzymatic Cleavage of Peptide Bonds: The Half-cystine Residues in Bovine Pancreatic Ribonuclease , 1964 .

[17]  A. Patchornik,et al.  Nonenzymatic Cleavages of Peptide Chains at the Cysteine and Serine Residues through Their Conversion to Dehydroalanine (DHAL). II. The Specific Chemical Cleavage of Cysteinyl Peptides , 1964 .

[18]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.

[19]  W. Rutter,et al.  Purification and properties of pigeon liver malic enzyme. , 1958, The Journal of biological chemistry.

[20]  J. Goa.  A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid. , 1953, Scandinavian journal of clinical and laboratory investigation.