Photobiological fundamentals of low-power laser therapy

Quantitative studies of the action of low-power visible monochromatic light on various cells (E. coli, yeasts, HeLa) were performed to find irradiation conditions (wavelength, dose, intensity) conducive to vital activity stimulation. The action spectra of visible light on DNA and RNA synthesis in HeLa cells have maxima near 404, 620, 680, 760, and 830 nm. Growth simulation of E. coli is at a maximum when irradiated at 404, 454, 570, 620, and 750 nm, and biomass accumulation stimulation in yeasts has a maxima at 404, 570, 620, 680, and 760 nm. Absorption of quanta is only a trigger for the rearrangement of cellular metabolism, with photosignal transduction being effected by standard cellular means such as changes in the cAMP level. Respiratory chain components are discussed as primary photoacceptors. It is concluded that "laser biostimulation" is of a photobiological nature, and low-power laser effects can be related to well-known photobiological phenomena.

[1]  R. Parshad,et al.  Intermittent exposure to fluorescent light extends lifespan of human diploid fibroblasts in culture , 1977, Nature.

[2]  B. Epel,et al.  Cytochrome a3: Destruction by Light , 1969, Science.

[3]  R. Tyrrell,et al.  LETHAL ACTION OF ULTRAVIOLET AND VISIBLE (BLUE‐VIOLET) RADIATIONS AT DEFINED WAVELENGTHS ON HUMAN LYMPHOBLASTOID CELLS: ACTION SPECTRA AND INTERACTION SITES , 1984, Photochemistry and photobiology.

[4]  R. Parshad,et al.  Proliferative response of human diploid fibroblasts to intermittent light exposure , 1977, Journal of cellular physiology.

[5]  H. Schairer,et al.  Electron-transport chains of Escherichia coli. Reconstitution of respiration in a 5-aminolaevulinic acid-requiring mutant. , 1973, European journal of biochemistry.

[6]  V. S. Letokhov,et al.  Biostimulation of HeLa cells by low-intensity visible light , 1984 .

[7]  R. Klein,et al.  INTERFERENCE BY NEAR ULTRAVIOLET AND GREEN LIGHT WITH GROWTH OF ANIMAL AND PLANT CELL CULTURES * , 1967, Photochemistry and photobiology.

[8]  V. Letokhov,et al.  Biostimulation of Hela cells by low-intensity visible light , 1984 .

[9]  S. A. Gordon,et al.  Red and far-red action on oxidative phosphorylation. , 1960, Radiation research.

[10]  B L Epel,et al.  Inhibition of growth and respiration by visible and near-visible light. , 1973, Photophysiology.

[11]  B. Aggarwal,et al.  Damage to mitochondrial electron transport and energy coupling by visible light. , 1978, Biochimica et biophysica acta.

[12]  Nikolai F. Gamaleya,et al.  Laser Biomedical Research in the USSR , 1977 .

[13]  G. Moreno,et al.  A study of beating frequency of a single myocardial cell. III. Laser micro-irradiation of mitochondria in the presence of KCN or ATP. , 1979, Experimental cell research.

[14]  J. Basford.  Low-energy laser treatment of pain and wounds: hype, hope, or hokum? , 1986, Mayo Clinic proceedings.

[15]  V. S. Letokhov,et al.  Biostimulation of HeLa cells by low-intensity visible light , 1982 .

[16]  E. Mester,et al.  Hypothetical physical model for laser biostimulation , 1982 .

[17]  W. Briggs,et al.  The Blue Light Receptor(s): Primary Reactions and Subsequent Metabolic Changes , 1981 .

[18]  J. Litwin.  The effect of light on the aging of human diploid fibroblasts. , 1972, Experimental gerontology.

[19]  J. G. Marshall,et al.  He-Ne laser stimulation of human fibroblast proliferation and attachment in vitro , 1986 .

[20]  J. Pouysségur,et al.  Cytoplasmic pH, a key determinant of growth factor‐induced DNA synthesis in quiescent fibroblasts , 1985, FEBS letters.

[21]  D. Rounds,et al.  The effect of intense visible light on cellular respiration. , 1967, Life sciences.

[22]  V. Letokhov,et al.  Effect of the He-Ne Laser Radiation on the Reproduction Rate and Protein Synthesis of the Yeast , 1984 .

[23]  G. Heldmaier,et al.  Photoperiodic control and effects of melatonin on nonshivering thermogenesis and brown adipose tissue. , 1981, Science.

[24]  I. Agrell.  Phytohaemagglutinin as a mitotic stimulator on free-living amoebae. , 1966, Experimental cell research.

[25]  H. Burki,et al.  Comparison of the lethal and mutagenic effects of gold and white fluorescent lights on cultured mammalian cells. , 1978, Mutation research.

[26]  R. Webb,et al.  SENSITIVITY OF STRAINS OF ESCHERICHIA COLI DIFFERING IN REPAIR CAPABILITY TO FAR UV, NEAR UV AND VISIBLE RADIATIONS * , 1976, Photochemistry and photobiology.

[27]  V. S. Letokhov,et al.  Stimulation of E. coli growth by laser and incoherent red light , 1983 .

[28]  J. Jagger.  Physiological Effects of Near-Ultraviolet Radiation on Bacteria , 1983 .

[29]  I. M. Catalano,et al.  Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium‐neon laser , 1984, FEBS letters.