Human endothelial and osteoblast co-cultures on 3D biomaterials.

Increasingly, in vitro experiments are being used to evaluate the cell compatibility of novel biomaterials. Single cell cultures have been used to determine how well cells attach, grow, and exhibit characteristic functions on these materials and the outcome of such tests is generally accepted as an indicator of biocompatibility. However, organs and tissues are not made up of one cell type and the interaction of cells is known to be an essential factor for physiological cell function. To more accurately examine biomaterials for bone regeneration, we have developed methods to coculture osteoblasts, which are the primary cell type making up bone, and endothelial cells, which form the vasculature supplying cells in the bone with oxygen and nutrients to survive on 2- and 3-D biomaterials.

[1]  D. Paul,et al.  Growth of human cells on polyethersulfone (PES) hollow fiber membranes. , 2005, Biomaterials.

[2]  Kirsten Peters,et al.  Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering. , 2004, Biomaterials.

[3]  Rui L Reis,et al.  Response of micro- and macrovascular endothelial cells to starch-based fiber meshes for bone tissue engineering. , 2007, Biomaterials.

[4]  C James Kirkpatrick,et al.  Microvessel-like structures from outgrowth endothelial cells from human peripheral blood in 2-dimensional and 3-dimensional co-cultures with osteoblastic lineage cells. , 2007, Tissue engineering.

[5]  J. L. Gomez Ribelles,et al.  Analysis of the biological response of endothelial and fibroblast cells cultured on synthetic scaffolds with various hydrophilic/hydrophobic ratios: influence of fibronectin adsorption and conformation. , 2009, Tissue engineering. Part A.

[6]  Quan Huang,et al.  Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes. , 2005, Biomaterials.

[7]  Claudio Migliaresi,et al.  Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells. , 2009, Biomaterials.

[8]  Veerle Cnudde,et al.  Porous gelatin hydrogels: 2. In vitro cell interaction study. , 2007, Biomacromolecules.

[9]  Kirsten Peters,et al.  Cell culture models of higher complexity in tissue engineering and regenerative medicine. , 2007, Biomaterials.

[10]  C. Kirkpatrick,et al.  Biocompatibility studies of endothelial cells on a novel calcium phosphate/SiO2-xerogel composite for bone tissue engineering , 2008, Biomedical materials.

[11]  C James Kirkpatrick,et al.  Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. , 2007, Biomaterials.

[12]  C James Kirkpatrick,et al.  In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R. , 2002, Microvascular research.

[13]  Erhan Piskin,et al.  Endothelial cell colonization and angiogenic potential of combined nano- and micro-fibrous scaffolds for bone tissue engineering. , 2008, Biomaterials.

[14]  C. Kirkpatrick,et al.  Functionality of endothelial cells on silk fibroin nets: comparative study of micro- and nanometric fibre size. , 2008, Biomaterials.

[15]  Rui L Reis,et al.  Contribution of outgrowth endothelial cells from human peripheral blood on in vivo vascularization of bone tissue engineered constructs based on starch polycaprolactone scaffolds. , 2009, Biomaterials.