Effects of Dietary Cholesterol and Its Oxidation Products on Pathological Lesions and Cholesterol and Lipid Oxidation in the Rabbit Liver

This study was conducted to determine the effects of dietary cholesterol (CHO) and cholesterol oxidation products (COPs) on the induction of pathological lesions in rabbit liver tissues. Liver lesions were induced only when the levels of CHO and COPs in the diet were very high. The amount of CHO measured in the liver increased when dietary CHO was increased; by comparison, dietary COPs affected liver CHO amounts to a lesser extent. The TBARS (thiobarbituric acid reactive substances) value measured for the liver samples also increased when dietary CHO and COP levels were elevated, and the TBARS value was more strongly affected by the amount of COPs in the diet than by the amount of CHO. At 6 and 12 weeks, COP levels were the highest in the group that received 1.2 g CHO + 0.8 g COPs, followed by the 0.5 g CHO + 0.5 g COPs and 1.6 g CHO + 0.4 g COPs groups; the control (0 g) group showed the lowest COP levels among all groups. In this study, we found that not only dietary CHO but also COPs were involved in hypercholesterolemia induced liver lesions when the amount of CHO and COPs was high.

[1]  Eun Joo Lee,et al.  Effect of Dietary Cholesterol and Cholesterol Oxides on Blood Cholesterol, Lipids, and the Development of Atherosclerosis in Rabbits , 2013, International journal of molecular sciences.

[2]  D. Ahn,et al.  Dietary cholesterol affects lipid metabolism in rabbits , 2013, Food Science and Biotechnology.

[3]  W. Marsden.  I and J , 2012 .

[4]  S. Subramanian,et al.  Dietary cholesterol exacerbates hepatic steatosis and inflammation in obese LDL receptor-deficient mice , 2011, Journal of Lipid Research.

[5]  A. Aghaei,et al.  A novel capacitive biosensor for cholesterol assay that uses an electropolymerized molecularly imprinted polymer , 2010 .

[6]  A. Vejux,et al.  Cytotoxic effects of oxysterols associated with human diseases: Induction of cell death (apoptosis and/or oncosis), oxidative and inflammatory activities, and phospholipidosis. , 2009, Molecular aspects of medicine.

[7]  M. Rekhter,et al.  Chemical analysis of atherosclerotic plaque cholesterol combined with histology of the same tissue Published, JLR Papers in Press, March 18, 2008. , 2008, Journal of Lipid Research.

[8]  Age K Smilde,et al.  Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis , 2007, Genome Biology.

[9]  K. Feingold,et al.  The role of dietary oxidized cholesterol and oxidized fatty acids in the development of atherosclerosis. , 2005, Molecular nutrition & food research.

[10]  M. V. Magni,et al.  The presence and the role of chromatin cholesterol in rat liver regeneration. , 2002, Journal of hepatology.

[11]  A. Girotti,et al.  Hyperresistance to cholesterol hydroperoxide-induced peroxidative injury and apoptotic death in a tumor cell line that overexpresses glutathione peroxidase isotype-4. , 2001, Free radical biology & medicine.

[12]  J. Sell,et al.  Analysis of cholesterol oxides in egg yolk and turkey meat. , 1999, Poultry science.

[13]  E. C. Lin,et al.  Regulation of guinea pig hepatic acyl-coa:cholesterol acyltransferase activity by dietary fat saturation and cholesterol. , 1999, The Journal of nutritional biochemistry.

[14]  J. Sawyer,et al.  Dietary monounsaturated fatty acids promote aortic atherosclerosis in LDL receptor-null, human ApoB100-overexpressing transgenic mice. , 1998, Arteriosclerosis, thrombosis, and vascular biology.

[15]  D. Ahn,et al.  Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. , 1998, Poultry science.

[16]  A. D. Jones,et al.  Cholesterol Oxides in Foods of Animal Origin , 1995 .

[17]  Rao Kn,et al.  The significance of the cholesterol biosynthetic pathway in cell growth and carcinogenesis (review). , 1995 .

[18]  K N Rao,et al.  The significance of the cholesterol biosynthetic pathway in cell growth and carcinogenesis (review). , 1995, Anticancer research.

[19]  J. Dietschy,et al.  Role of liver in the maintenance of cholesterol and low density lipoprotein homeostasis in different animal species, including humans. , 1993, Journal of lipid research.

[20]  H. Weisman,et al.  Reduction of atherosclerosis by administration of dehydroepiandrosterone. A study in the hypercholesterolemic New Zealand white rabbit with aortic intimal injury. , 1988, The Journal of clinical investigation.

[21]  B. Hennig,et al.  Cholestan-3gb,5α,6β-triol decreases barrier function of cultured endothelial cell monolayers , 1987 .

[22]  L. Smith,et al.  Cholesterol autoxidation 1981-1986. , 1987, Chemistry and physics of lipids.

[23]  Leland L. Smith.  Cholesterol Autoxidation , 1981, Springer US.

[24]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[25]  H. Weisman,et al.  Reduction of Atherosclerosis by Administration of Dehydroepiandrosterone , 2022 .