High Level Expression of Recombinant Genes in Aspergillus Oryzae

We have modified a method for transformation of Aspergillus nidulans1 to work for Aspergillus oryzae using selection for the amdS and argB genes from Aspergillus nidulans. To direct the expression of recombinant genes in A. oryzae we have used an alpha-amylase promoter cloned from a high yielding strain of A. oryzae. The amounts of heterologous protein obtained make this system attractive for even moderately priced industrial enzymes. As an example, the Rhizomucor miehei derived aspartic proteinase was secreted with yields in excess of three grams per liter. The proteinase was slightly overglycosylated, which did not, however, alter the specific activity of the enzyme.

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