Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope

Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.

[1]  Jacob N. Israelachvili,et al.  Intermolecular and surface forces : with applications to colloidal and biological systems , 1985 .

[2]  Galen D. Stucky,et al.  Progress in sequencing deoxyribonucleic acid with an atomic force microscope , 1991 .

[3]  C. Bustamante,et al.  Circular DNA molecules imaged in air by scanning force microscopy. , 1992, Biochemistry.

[4]  C F Quate,et al.  Imaging crystals, polymers, and processes in water with the atomic force microscope. , 1989, Science.

[5]  Gerd Binnig,et al.  Atomic Resolution with Atomic Force Microscope , 1987 .

[6]  G. Binnig,et al.  Two-dimensional ordering of the DNA base guanine observed by scanning tunneling microscopy. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[7]  H. Murakami,et al.  New scanning tunneling microscopy tip for measuring surface topography , 1990 .

[8]  T. Beebe,et al.  Graphite: a mimic for DNA and other biomolecules in scanning tunneling microscope studies. , 1991, Science.

[9]  T. Thundat,et al.  Images of the DNA double helix in water. , 1989, Science.

[10]  R Balhorn,et al.  Direct observation of native DNA structures with the scanning tunneling microscope. , 1989, Science.

[11]  C. Bustamante,et al.  Images of single-stranded nucleic acids by scanning tunnelling microscopy , 1989, Nature.

[12]  Tersoff,et al.  Tunneling microscopy study of the graphite surface in air and water. , 1986, Physical review. B, Condensed matter.

[13]  J. Baldeschwieler,et al.  Atomic-scale imaging of DNA using scanning tunnelling microscopy , 1990, Nature.

[14]  H. Bujard,et al.  An electron microscopic method for studying nucleic acid–protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3 , 1974, Biopolymers.

[15]  A. Bard,et al.  Large Scale Hexagonal Domainlike Structures Superimposed on the Atomic Corrugation of a Graphite Surface Observed by Scanning Tunneling Microscopy , 1991 .

[16]  Scanning tunneling microscopy of nucleic acids. , 1989, Methods in enzymology.

[17]  B. Levi,et al.  Special Issue: Communicating Physics to the Public , 1990 .

[18]  Paul K. Hansma,et al.  Molecular-resolution images of Langmuir-Blodgett films and DNA by atomic force microscopy , 1991 .

[19]  R. Lal,et al.  Atomic force microscopy and dissection of gap junctions , 1991, Science.

[20]  M. J. Vasile,et al.  Scanning probe tips formed by focused ion beams , 1991 .

[21]  T. Kunisada,et al.  Rapid microscale procedure for visualizing intracellular plasmid DNA by electron microscopy. , 1983, Plasmid.

[22]  C. Quate,et al.  Atomic resolution with the atomic force microscope on conductors and nonconductors , 1988 .

[23]  C. Quate,et al.  Forces in atomic force microscopy in air and water , 1989 .

[24]  K. L. Lee,et al.  Direct electron‐beam patterning for nanolithography , 1989 .