Fabrication and characters of a corneal endothelial cells scaffold based on chitosan

A novel chitosan-based membrane that made of hydroxyethyl chitosan, gelatin and chondroitin sulfate was used as a carrier of corneal endothelial cells. The characteristics of the blend membrane including transparency, equilibrium water content, ion and glucose permeability were determined. The results showed that the optical transparency of the membrane was as good as the natural human cornea. The water content of this scaffold was 81.32% which was remarkably close to the native cornea. The membrane had a good ion permeability and its glucose permeability was even higher than natural human cornea. The cultured rabbit corneal endothelial cells formed a monolayer on the membrane. The results demonstrated that the membrane was suitable for corneal endothelial cells to attach and grow on it. In addition, the membranes in vivo could be degraded steadily with less inflammation and showed a good histocompatibility. These results demonstrated that the hydroxyethyl chitosan-chondroitin sulfate-gelatin blend membrane can potentially be used as a carrier for corneal endothelial cell transplantation.

[1]  B. Strates,et al.  Chemically modified collagen: a natural biomaterial for tissue replacement. , 1987, Journal of biomedical materials research.

[2]  S. Keipert,et al.  In vitro permeation studies comparing bovine nasal mucosa, porcine cornea and artificial membrane: androstenedione in microemulsions and their components. , 2004, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[3]  T V Chirila,et al.  An overview of the development of artificial corneas with porous skirts and the use of PHEMA for such an application. , 2001, Biomaterials.

[4]  Jau-Song Yu,et al.  Regulation of corneal angiogenesis in limbal stem cell deficiency , 2006, Progress in Retinal and Eye Research.

[5]  C. Siedlecki,et al.  Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. , 2007, Biomaterials.

[6]  S. Tokura,et al.  Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro. , 1997, Biomaterials.

[7]  B. Han,et al.  Study on a hydroxypropyl chitosan–gelatin based scaffold for corneal stroma tissue engineering , 2009 .

[8]  Masayuki Yamato,et al.  Reconstruction of functional tissues with cell sheet engineering. , 2007, Biomaterials.

[9]  I J Constable,et al.  Cell viability and inflammatory response in hydrogel sponges implanted in the rabbit cornea. , 1998, Biomaterials.

[10]  Chad Johnson,et al.  The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. , 2004, Biomaterials.

[11]  E. Orwin,et al.  In vitro culture characteristics of corneal epithelial, endothelial, and keratocyte cells in a native collagen matrix. , 2000, Tissue engineering.

[12]  J. Suh,et al.  Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. , 2000, Biomaterials.

[13]  A. Lloyd,et al.  Ocular biomaterials and implants. , 2001, Biomaterials.

[14]  B. Han,et al.  Preparation and properties of a chitosan-based carrier of corneal endothelial cells , 2008, Journal of materials science. Materials in medicine.

[15]  P. Eggli,et al.  Morphological and functional analysis of immortalized human corneal endothelial cells after transplantation. , 1999, Experimental eye research.

[16]  D. Drexler,et al.  Transplantation of adult human or porcine corneal endothelial cells onto human recipients in vitro. Part I: Cell culturing and transplantation procedure. , 1999, Cornea.

[17]  T. Wood,et al.  Transplantation of Corneal Endothelial Cells Using a Cell Carrier Device , 1994, Cornea.

[18]  M. Araie,et al.  Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model. , 2004, Investigative ophthalmology & visual science.

[19]  W. Friess,et al.  Collagen – biomaterial for drug delivery 1 , 1998 .

[20]  N. Peppas,et al.  Structure and Interactions in Covalently and Ionically Crosslinked Chitosan Hydrogels for Biomedical Applications , 2003 .

[21]  N. Fullwood,et al.  Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation. , 2004, Investigative ophthalmology & visual science.

[22]  M. Menger,et al.  New experimental approach to study host tissue response to surgical mesh materials in vivo. , 2005, Journal of biomedical materials research. Part A.

[23]  W. Liu,et al.  Application of scaffold materials in tissue reconstruction in immunocompetent mammals: our experience and future requirements. , 2007, Biomaterials.

[24]  J. Vacanti,et al.  Ocular cell monolayers cultured on biodegradable substrates. , 1999, Tissue engineering.

[25]  K. Dobbins,et al.  Trends in the Indications for Penetrating Keratoplasty in the Midwestern United States , 2000, Cornea.

[26]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[27]  T. Okano,et al.  Structural characterization of bioengineered human corneal endothelial cell sheets fabricated on temperature-responsive culture dishes. , 2006, Biomaterials.

[28]  Yao-xiong Huang,et al.  Study on biocompatibility of complexes of collagen-chitosan-sodium hyaluronate and cornea. , 2005, Artificial organs.

[29]  Kanji Tsuru,et al.  Synthesis and cytocompatibility of porous chitosan–silicate hybrids for tissue engineering scaffold application , 2008 .

[30]  Fengfu Li,et al.  PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering. , 2008, Biomaterials.

[31]  B. McCarey,et al.  Modeling glucose distribution in the cornea. , 1990, Current eye research.

[32]  J. Heller,et al.  Lysozyme degradation of partially deacetylated chitin, its films and hydrogels. , 1982, Biomaterials.