Macroporous nanowire nanoelectronic scaffolds for synthetic tissues.

The development of three-dimensional (3D) synthetic biomaterials as structural and bioactive scaffolds is central to fields ranging from cellular biophysics to regenerative medicine. As of yet, these scaffolds cannot electrically probe the physicochemical and biological microenvironments throughout their 3D and macroporous interior, although this capability could have a marked impact in both electronics and biomaterials. Here, we address this challenge using macroporous, flexible and free-standing nanowire nanoelectronic scaffolds (nanoES), and their hybrids with synthetic or natural biomaterials. 3D macroporous nanoES mimic the structure of natural tissue scaffolds, and they were formed by self-organization of coplanar reticular networks with built-in strain and by manipulation of 2D mesh matrices. NanoES exhibited robust electronic properties and have been used alone or combined with other biomaterials as biocompatible extracellular scaffolds for 3D culture of neurons, cardiomyocytes and smooth muscle cells. Furthermore, we show the integrated sensory capability of the nanoES by real-time monitoring of the local electrical activity within 3D nanoES/cardiomyocyte constructs, the response of 3D-nanoES-based neural and cardiac tissue models to drugs, and distinct pH changes inside and outside tubular vascular smooth muscle constructs.

[1]  G. Smirnov,et al.  Possibilities and Limitations , 1970 .

[2]  John A. Kiernan,et al.  Histological and Histochemical Methods: Theory and Practice , 1981 .

[3]  M. Lings,et al.  Articles , 1967, Soil Science Society of America Journal.

[4]  O. Prohaska,et al.  Thin-Film Multiple Electrode Probes: Possibilities and Limitations , 1986, IEEE Transactions on Biomedical Engineering.

[5]  J. Jalife,et al.  Cardiac Electrophysiology: From Cell to Bedside , 1990 .

[6]  F A Auger,et al.  A completely biological tissue‐engineered human blood vessel , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  Miguel A. L. Nicolelis,et al.  Methods for Neural Ensemble Recordings , 1998 .

[8]  I. LeGrice,et al.  3‐Dimensional configuration of perimysial collagen fibres in rat cardiac muscle at resting and extended sarcomere lengths , 1999, The Journal of physiology.

[9]  Rebecca S. Shawgo,et al.  Biocompatibility and biofouling of MEMS drug delivery devices. , 2003, Biomaterials.

[10]  Yi Cui,et al.  Controlled Growth and Structures of Molecular-Scale Silicon Nanowires , 2004 .

[11]  Charles M. Lieber,et al.  Encoding Electronic Properties by Synthesis of Axial Modulation-Doped Silicon Nanowires , 2005, Science.

[12]  Resident neuroelectrochemical interfacing using carbon nanofiber arrays. , 2006, The journal of physical chemistry. B.

[13]  N. L'Heureux,et al.  Human tissue-engineered blood vessels for adult arterial revascularization , 2007, Nature Medicine.

[14]  H J Adrogué,et al.  Metabolic acidosis: pathophysiology, diagnosis and management. , 2006, Journal of nephrology.

[15]  Charles M. Lieber,et al.  Nanoelectronics from the bottom up. , 2007, Nature materials.

[16]  M. Ericson,et al.  Vertically aligned carbon nanofiber arrays record electrophysiological signals from hippocampal slices. , 2007, Nano letters.

[17]  Miguel A. L. Nicolelis.  Methods for Neural Ensemble Recordings, Second Edition , 2007 .

[18]  L. Cauller,et al.  Biocompatible SU-8-Based Microprobes for Recording Neural Spike Signals From Regenerated Peripheral Nerve Fibers , 2008, IEEE Sensors Journal.

[19]  T. Someya,et al.  A Rubberlike Stretchable Active Matrix Using Elastic Conductors , 2008, Science.

[20]  Lisa E. Freed,et al.  Accordion-Like Honeycombs for Tissue Engineering of Cardiac Anisotropy , 2008, Nature materials.

[21]  B. Ziaie,et al.  Enhanced 3-D Folding of Silicon Microstructures via Thermal Shrinkage of a Composite Organic/Inorganic Bilayer , 2008, Journal of Microelectromechanical Systems.

[22]  Claire Wyart,et al.  Colloid-guided assembly of oriented 3D neuronal networks , 2008, Nature Methods.

[23]  John A. Kiernan,et al.  Histological and Histochemical Methods: Theory and Practice, 4th edition , 2008 .

[24]  Bozhi Tian,et al.  Single crystalline kinked semiconductor nanowire superstructures , 2009, Nature nanotechnology.

[25]  Charles M Lieber,et al.  Flexible electrical recording from cells using nanowire transistor arrays , 2009, Proceedings of the National Academy of Sciences.

[26]  T. Boland,et al.  Electrophysiological characterization of embryonic hippocampal neurons cultured in a 3D collagen hydrogel. , 2009, Biomaterials.

[27]  Kristi S. Anseth,et al.  Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties , 2009, Science.

[28]  Charles M. Lieber,et al.  Electrical recording from hearts with flexible nanowire device arrays. , 2009, Nano letters.

[29]  Molly M Stevens,et al.  Synthetic polymer scaffolds for tissue engineering. , 2009, Chemical Society reviews.

[30]  Dielectric Layers.  Highly Sensitive Flexible Pressure Sensors with Micro-structured Rubber , 2010 .

[31]  D. Ingber,et al.  Reconstituting Organ-Level Lung Functions on a Chip , 2010, Science.

[32]  Nicholas V. Annetta,et al.  A Conformal, Bio-Interfaced Class of Silicon Electronics for Mapping Cardiac Electrophysiology , 2010, Science Translational Medicine.

[33]  C. Lieber,et al.  Design and Implementation of Functional Nanoelectronic Interfaces With Biomolecules, Cells, and Tissue Using Nanowire Device Arrays , 2010, IEEE Transactions on Nanotechnology.

[34]  Benjamin C. K. Tee,et al.  Highly sensitive flexible pressure sensors with microstructured rubber dielectric layers. , 2010, Nature materials.

[35]  Bozhi Tian,et al.  Nanowire transistor arrays for mapping neural circuits in acute brain slices , 2010, Proceedings of the National Academy of Sciences.

[36]  Dietmar W Hutmacher,et al.  Biomaterials offer cancer research the third dimension. , 2010, Nature materials.

[37]  Charles M. Lieber,et al.  Three-Dimensional, Flexible Nanoscale Field-Effect Transistors as Localized Bioprobes , 2010, Science.

[38]  Andrew G. Gillies,et al.  Nanowire active-matrix circuitry for low-voltage macroscale artificial skin. , 2010, Nature materials.

[39]  S. Downes,et al.  Aligned electrospun polymer fibres for skeletal muscle regeneration. , 2010, European cells & materials.

[40]  L. Goldstein,et al.  Decellularized porcine brain matrix for cell culture and tissue engineering scaffolds. , 2011, Tissue engineering. Part A.

[41]  Kyungsu Na,et al.  Directing Zeolite Structures into Hierarchically Nanoporous Architectures , 2011, Science.

[42]  Tal Dvir,et al.  Nanotechnological strategies for engineering complex tissues. , 2020, Nature nanotechnology.

[43]  Monya Baker,et al.  Tissue models: A living system on a chip , 2011, Nature.

[44]  J. Rogers,et al.  Materials for multifunctional balloon catheters with capabilities in cardiac electrophysiological mapping and ablation therapy. , 2011, Nature materials.

[45]  Claudiu T. Supuran,et al.  Interfering with pH regulation in tumours as a therapeutic strategy , 2011, Nature Reviews Drug Discovery.

[46]  Robert Langer,et al.  Three-dimensional biomaterials for the study of human pluripotent stem cells , 2011, Nature Methods.

[47]  L. Valdevit,et al.  Ultralight Metallic Microlattices , 2011, Science.

[48]  Seeram Ramakrishna,et al.  Electrospun biocomposite nanofibrous patch for cardiac tissue engineering , 2011, Biomedical materials.

[49]  Hao Yan,et al.  Programmable nanowire circuits for nanoprocessors , 2011, Nature.

[50]  Tal Dvir,et al.  Nanowired three dimensional cardiac patches , 2011, Nature nanotechnology.

[51]  Raeed H. Chowdhury,et al.  Epidermal Electronics , 2011, Science.

[52]  J. Collins,et al.  Synthetic Biology Moving into the Clinic , 2011, Science.

[53]  P. Schwille.  Bottom-Up Synthetic Biology: Engineering in a Tinkerer’s World , 2011, Science.

[54]  Smadar Cohen,et al.  Integration of multiple cell-matrix interactions into alginate scaffolds for promoting cardiac tissue regeneration. , 2011, Biomaterials.

[55]  Brian Litt,et al.  Flexible, Foldable, Actively Multiplexed, High-Density Electrode Array for Mapping Brain Activity in vivo , 2011, Nature Neuroscience.

[56]  Cindi M Morshead,et al.  Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels. , 2011, Nature materials.