New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples

ABSTRACT Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

[1]  A. Pospischil,et al.  Murine model of pneumonia caused by Parachlamydia acanthamoebae. , 2008, Microbial pathogenesis.

[2]  Gilbert GREUB,et al.  Protochlamydia naegleriophila as Etiologic Agent of Pneumonia , 2008, Emerging infectious diseases.

[3]  Gilbert GREUB,et al.  Criblamydia sequanensis, a new intracellular Chlamydiales isolated from Seine river water using amoebal co-culture. , 2006, Environmental microbiology.

[4]  Gilbert GREUB,et al.  Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. , 2006, Microbes and infection.

[5]  Gilbert GREUB,et al.  Pathogenic Potential of Novel Chlamydiae and Diagnostic Approaches to Infections Due to These Obligate Intracellular Bacteria , 2006, Clinical Microbiology Reviews.

[6]  D. Raoult,et al.  Intracellular trafficking of Parachlamydia acanthamoebae , 2005, Cellular microbiology.

[7]  Gilbert Greub,et al.  Amoebae-resisting bacteria isolated from human nasal swabs by amoebal coculture. , 2004, Emerging infectious diseases.

[8]  D. Raoult,et al.  Parachlamydia acanthamoebae Enters and Multiplies within Human Macrophages and Induces Their Apoptosis , 2004, Infection and Immunity.

[9]  D. Raoult,et al.  Parachlamydia acanthamoeba Enters and Multiplies within Human Macrophages and Induces Their Apoptosis , 2003, Infection and Immunity.

[10]  D. Raoult,et al.  Serological Hint Suggesting That Parachlamydiaceae Are Agents of Pneumonia in Polytraumatized Intensive Care Patients , 2003, Annals of the New York Academy of Sciences.

[11]  D. Raoult,et al.  Parachlamydiaceae as Rare Agents of Pneumonia , 2003, Emerging infectious diseases.

[12]  D. Greenberg,et al.  High Rate of Simkania negevensis among Canadian Inuit Infants Hospitalized with Lower Respiratory Tract Infections , 2003, Scandinavian journal of infectious diseases.

[13]  D. Raoult,et al.  Parachlamydiaceae: Potential Emerging Pathogens , 2002, Emerging infectious diseases.

[14]  T. Marrie,et al.  Legionella-like and other amoebal pathogens as agents of community-acquired pneumonia. , 2001, Emerging infectious diseases.

[15]  A. L. Le Faou,et al.  A new chlamydia-like 16S rDNA sequence from a clinical sample. , 2001, Microbiology.

[16]  M. Shimada,et al.  High Prevalence of Wolbachia in the Azuki Bean Beetle Callosobruchus chinensis (Coleoptera, Bruchidae) , 1999 .

[17]  D. Greenberg,et al.  High prevalence of "Simkania Z," a novel Chlamydia-like bacterium, in infants with acute bronchiolitis. , 1998, The Journal of infectious diseases.

[18]  T. Marrie,et al.  Chlamydia-like obligate parasite of free-living amoebae , 1997, The Lancet.

[19]  R. Amann,et al.  Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp , 1997, Applied and environmental microbiology.