In vitro infection of aortic endothelial cells by caprine arthritis encephalitis virus enhances in vitro transmigration of peripheral blood leukocytes and modulates their phenotypic expression.

Infection of goats by caprine arthritis-encephalitis virus (CAEV) provides a convenient example of the infiltration of various tissues by leukocytes following a natural lentiviral infection. This event is important in determining organ susceptibility and local immunity. Caprine vascular endothelial cells are susceptible to infection by CAEV in vitro, so we have investigated the consequences of this infection on the transmigration of uninfected leukocytes in an in vitro model. After in vitro infection by CAEV or stimulation by TNFalpha, the endothelial cells allowed the passage of tenfold more leukocytes from uninfected donors than did the uninfected endothelial cells. The transmigrating leukocytes were enriched in CD8+ lymphocytes, and the leukocytes appeared to have been activated during transmigration, as demonstrated by their expression of IL2R, MHC class II antigens and gamma-delta T-lymphocyte markers. CD4+, CD8+ and B-lymphocytes all proliferated in culture after transmigration. These results suggest that any possible infection or specific stimulation of endothelia in an infected animal could profoundly influence the choice of target organs and could activate the cells involved in local mucosal immune responses.

[1]  H. Garnett,et al.  Interleukin-1 production and cell-activation response to cytomegalovirus infection of vascular endothelial cells , 2005, Archives of Virology.

[2]  D. Kreisel,et al.  Multiparameter flow cytometric approach for simultaneous evaluation of T lymphocyte-endothelial cell interactions. , 2001, Cytometry.

[3]  C. Baldwin,et al.  Evaluation of cell replication by bovine T cells in polyclonally activated cultures using carboxyfluorescein succinimidyl ester (CFSE) loading and flow cytometric analysis. , 2000, Research in veterinary science.

[4]  E. Peterhans,et al.  B-cell epitopes of the envelope glycoprotein of caprine arthritis-encephalitis virus and antibody response in infected goats. , 2000, The Journal of general virology.

[5]  T. Greenland,et al.  Activation of small ruminant aortic endothelial cells after in vitro infection by caprine arthritis encephalitis virus. , 2000, Research in veterinary science.

[6]  A. Oxenius,et al.  Cytotoxic T Lymphocyte Responses to Human Immunodeficiency Virus: Control and Escape , 2000, Stem cells.

[7]  S. Carding,et al.  The importance of gd T cells in the resolution of pathogen‐induced inflammatory immune responses , 2000 .

[8]  S. Carding,et al.  The importance of gamma delta T cells in the resolution of pathogen-induced inflammatory immune responses. , 2000, Immunological reviews.

[9]  S. Kostense,et al.  Longitudinal Phenotypic Analysis of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes: Correlation with Disease Progression , 1999, Journal of Virology.

[10]  M. Yáñez-Mó,et al.  Activation of peripheral blood T cells by interaction and migration through endothelium: role of lymphocyte function antigen-1/intercellular adhesion molecule-1 and interleukin-15. , 1999, Blood.

[11]  M. Yacoub,et al.  The effects of monocytes on the transendothelial migration of T lymphocytes , 1998, Immunology.

[12]  E. Peterhans,et al.  Maedi-visna virus infection in sheep: a review. , 1998, Veterinary research.

[13]  R. Brezinschek,et al.  Interleukin 15 is produced by endothelial cells and increases the transendothelial migration of T cells In vitro and in the SCID mouse-human rheumatoid arthritis model In vivo. , 1998, The Journal of clinical investigation.

[14]  H. Gudmundsdottir,et al.  Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. , 1997, The Journal of clinical investigation.

[15]  N. King,et al.  Early E-selectin, VCAM-1, ICAM-1, and late major histocompatibility complex antigen induction on human endothelial cells by flavivirus and comodulation of adhesion molecule expression by immune cytokines , 1997, Journal of virology.

[16]  E. A. Wright,et al.  In vitro transendothelial migration of blood T lymphocytes from HIV‐infected individuals , 1997, AIDS.

[17]  Y. Chebloune,et al.  Variations in lentiviral gene expression in monocyte-derived macrophages from naturally infected sheep. , 1996, The Journal of general virology.

[18]  M. Hamilton,et al.  Analysis of monoclonal antibodies specific for the gamma delta TcR. , 1996, Veterinary immunology and immunopathology.

[19]  J. Ellis,et al.  Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes. , 1996, Veterinary immunology and immunopathology.

[20]  I. Hewlett,et al.  HIV-1-Tat protein promotes chemotaxis and invasive behavior by monocytes. , 1996, Journal of immunology.

[21]  O. Bagasra,et al.  Cellular reservoirs of HIV‐1 in the central nervous system of infected individuals: identification by the combination of in situ polymerase chain reaction and immunohistochemistry , 1996, AIDS.

[22]  H. Gendelman,et al.  Mechanisms for the transendothelial migration of HIV-1-infected monocytes into brain. , 1996, Journal of immunology.

[23]  J. Clements,et al.  Molecular biology and pathogenesis of animal lentivirus infections , 1996, Clinical microbiology reviews.

[24]  F. Guiguen,et al.  Histogenesis of the pulmonary lesions in the course of visna maedi virus-induced pneumonia. , 1996, Veterinary research.

[25]  J. Clements,et al.  Neurovirulent simian immunodeficiency virus replicates productively in endothelial cells of the central nervous system in vivo and in vitro , 1994, Journal of virology.

[26]  M. Zink,et al.  Pathobiology of lentivirus infections of sheep and goats. , 1994, Virus research.

[27]  C. Lichtensteiger,et al.  CD8+ cytotoxic T lymphocytes against antigenic variants of caprine arthritis-encephalitis virus. , 1993, The Journal of general virology.

[28]  C. Wiley,et al.  Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. , 1986, Proceedings of the National Academy of Sciences of the United States of America.