Adhesion of different bacterial strains to low-temperature plasma treated biomedical PVC catheter surfaces.

In this study, firstly five different bacteria (i.e. Coagulase positive and negative staphylococcus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa) with their different strains were isolated and used. The contact angle, surface free energy, p-xylene adhesion, and zeta potential of these bacteria were in the range of 43-69 deg, 45.4-61.8 erg cm(-2), 2.3-80.3%, and from -650.2 to + 17.5 mV, respectively. Most of the bacteria were negatively charged. Attachment of these bacteria to PVC catheter and its DMAEMA- and AAc-plasma treated forms were investigated. Bacterial attachment to the hydrophobic PVC catheter was high. Both plasma treatments caused significant drops in bacterial attachment in most of the cases. The effects of AAc-plasma treatment was more significant.

[1]  Paul Rouxhet,et al.  Methods for Measuring Hydrophobicity of Microorganisms , 1987 .

[2]  P. Sohnle,et al.  In vitro quantitative adherence of bacteria to intravascular catheters. , 1983, The Journal of surgical research.

[3]  D. Williams,et al.  Effects of physical configuration and chemical structure of suture materials on bacterial adhesion. A possible link to wound infection. , 1984, American journal of surgery.

[4]  P. Sohnle,et al.  Persistent in vitro survival of coagulase-negative staphylococci adherent to intravascular catheters in the absence of conventional nutrients , 1986, Journal of clinical microbiology.

[5]  F. Millero,et al.  Electrolyte Effects on Attachment of an Estuarine Bacterium , 1984, Applied and environmental microbiology.

[6]  T. Stenström,et al.  Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles , 1989, Applied and environmental microbiology.

[7]  J. Feijen,et al.  Adhesion of Escherichia coli on to a series of poly(methacrylates) differing in charge and hydrophobicity. , 1991, Biomaterials.

[8]  G. Peters,et al.  Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. , 1982, The Journal of infectious diseases.

[9]  H. Busscher,et al.  A COMPARISON OF VARIOUS METHODS TO DETERMINE HYDROPHOBIC PROPERTIES OF STREPTOCOCCAL CELL-SURFACES , 1987 .

[10]  W. Zingg,et al.  Surface thermodynamics of bacterial adhesion , 1983, Applied and environmental microbiology.

[11]  Á. Pascual,et al.  Effect of plastic catheter material on bacterial adherence and viability. , 1991, Journal of medical microbiology.

[12]  Y. Miyake,et al.  Hydrophobic interaction in Candida albicans and Candida tropicalis adherence to various denture base resin materials , 1985, Infection and immunity.

[13]  J. Feijen,et al.  Adhesion of coagulase-negative staphylococci to methacrylate polymers and copolymers. , 1986, Journal of biomedical materials research.

[14]  A. Fattom,et al.  Hydrophobicity as an Adhesion Mechanism of Benthic Cyanobacteria , 1984, Applied and environmental microbiology.

[15]  W. Stamm,et al.  Prevention of catheter-associated urinary tract infection with a silver oxide-coated urinary catheter: clinical and microbiologic correlates. , 1990, The Journal of infectious diseases.

[16]  S. Ashkenazi,et al.  Adherence of Bacteria to Pediatric Intravenous Catheters and Needles and Its Relation to Phlebitis in Animals , 1984, Pediatric Research.

[17]  H. C. van der Mei,et al.  Surface properties of Streptococcus salivarius HB and nonfibrillar mutants: measurement of zeta potential and elemental composition with X-ray photoelectron spectroscopy , 1988, Journal of bacteriology.

[18]  Eugene Rosenberg,et al.  Adherence of bacteria to hydrocarbons: A simple method for measuring cell‐surface hydrophobicity , 1980 .

[19]  G. Erdos,et al.  The production of antibacterial tubing, sutures, and bandages by in situ precipitation of metallic salts. , 1991, Canadian journal of microbiology.

[20]  H. Busscher,et al.  Effect of Zeta Potential and Surface Energy on Bacterial Adhesion to Uncoated and Saliva-coated Human Enamel and Dentin , 1988, Journal of dental research.

[21]  Y. Miyake,et al.  Cell-surface hydrophobicity of Candida species as determined by the contact-angle and hydrocarbon-adherence methods. , 1986, Journal of general microbiology.

[22]  G. Golomb,et al.  Prevention of bacterial colonization on polyurethane in vitro by incorporated antibacterial agent. , 1991, Journal of Biomedical Materials Research.

[23]  M. V. van Loosdrecht,et al.  Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion , 1987, Applied and environmental microbiology.

[24]  G. Veenstra,et al.  Ultrastructural organization and regulation of a biomaterial adhesin of Staphylococcus epidermidis , 1996, Journal of bacteriology.

[25]  L. Peterson,et al.  Effect of the ratio of surface area to volume on the penetration of antibiotics in to extravascular spaces in an in vitro model. , 1982, The Journal of infectious diseases.

[26]  A. Neumann,et al.  An equation-of-state approach to determine surface tensions of low-energy solids from contact angles , 1974 .