Effect of Medium Composition and Growth Regulators on Mass Propagation of Virus-Free Plant from the Meristem Cultures of 'Jarang' Grape

Abstract - This study was performed to clarify the effect of medium compositions and plant growth regulators on the shoot, root formation and growth of ‘Jarang’ grape for mass propagation of virus‐free plant. The formation and growth of shoot were considerably favorable in half ‐concentration of MS medium. However, the fo rmation of adventitious root per explants (avg. 2.1) was effective in higher concentration (two times) of MS medium. For sucrose concentration, 1% for the shoot formation, 3% for the adventitious root formation and 1% for the growth were observed as yield significant results. With the addition of 0.05% of activated carbon, the shoot growth was improved, and it was effective for the adventitious root formation and growth as well. A pH of 6.8 in the medium was the most suitable for mass propagation; the results showed significant enhancement in the number of nodes and the length of the shoot, 3.9 and 1.3 cm, respectively. The shoot growth was the most vigorous in BA 1.0 mg/L due to the impact of the growth regulator on the mass propagation in it. Consequently, 16.9 shoots per explant were formed in NAA 1.0 mg/L so good results were obtained.

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