Antibacterial and cytotoxicity activity in macroalgae extracts: perspectives for the use against pathogenic bacteria from shrimp farms ( Litopenaeus vannamei )

Some infections caused by pathogenic microorganisms might shows high prevalence in farmed shrimp environments, compromising production and causing economic losses. Therefore, the search for compounds with antibiotic activity has become intensive, following the record of new antimicrobial-resistant bacteria. The study of those bioactive compounds in marine macroalgae has produced satisfactory results, such as the discovery of antibacterial activity against multiresistant strains. Accordingly, this study aims to research antibiotic activity in macroalgae extracts of Chlorophyta, Phaeophyta and Rhodophyta found in the coast of Ceara and also to evaluate the cytotoxicity activity against bacterial strains ( Vibrio sp.) from shrimp farms ( Litopenaeus vannamei ). The extracts cytotoxicity was also evaluated. The results prove that there was antibacterial activity in ethanolic, acetonic, hexanic and methanolic extracts against bacterial strains of Vibrio with multiple resistance profile as well as displaying low cytotoxicity.

[1]  Hanaâ Zbakh,et al.  ANTIBACTERIAL ACTIVITY OF BENTHIC MARINE ALGAE EXTRACTS FROM THE MEDITERRANEAN COAST OF MOROCCO , 2020 .

[2]  Celso Alves,et al.  In vitro activities of kappa-carrageenan isolated from red marine alga Hypnea musciformis: Antimicrobial, anticancer and neuroprotective potential. , 2018, International journal of biological macromolecules.

[3]  P. Mittraparp-arthorn,et al.  Antibacterial and antioxidant activities of differential solvent extractions from the green seaweed Ulva intestinalis , 2017 .

[4]  D. Lenz,et al.  Brown seaweed Padina gymnospora is a prominent natural wound-care product , 2016 .

[5]  S. M. Mohy El-Din,et al.  Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea) , 2016 .

[6]  M. Lacroix,et al.  Antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens. , 2016, Food chemistry.

[7]  M. El-sheekh,et al.  Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria , 2016 .

[8]  A. Higuchi,et al.  Characterization and biotoxicity of Hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against Aedes aegypti and Plutella xylostella. , 2015, Ecotoxicology and environmental safety.

[9]  A. Vasconcelos,et al.  POLISSACARÍDEOS EXTRAÍDOS DE ALGAS MARINHAS E SUAS APLICAÇÕES BIOTECNOLÓGICAS: UMA REVISÃO , 2015 .

[10]  M. Guedes,et al.  Antiviral and Antioxidant Activities of Sulfated Galactomannans from Plants of Caatinga Biome , 2015, Evidence-based complementary and alternative medicine : eCAM.

[11]  P. Vos,et al.  Characterization of the virulence of Harveyi clade vibrios isolated from a shrimp hatchery in vitro and in vivo, in a brine shrimp (Artemia franciscana) model system , 2015 .

[12]  Xiaojing Zhang,et al.  Isolation and Characterization of Vibrio parahaemolyticus and Vibrio rotiferianus Associated with Mass Mortality of Chinese Shrimp (Fenneropenaeus chinensis) , 2014 .

[13]  N. Abdel-Raouf,et al.  Antibacterial substances from marine algae isolated from Jeddah coast of Red sea, Saudi Arabia. , 2014, Saudi journal of biological sciences.

[14]  E. Kamrani,et al.  The effects of brown alga, Sargassum glaucescensas (Agardeh, 1948) against selected bacterial, fungal and yeast pathogens of shrimp. , 2014 .

[15]  R. Paulert,et al.  Potencialidades das Macroalgas Marinhas na Agricultura: Revisão , 2013 .

[16]  G. C. Silva,et al.  Tropical Atlantic marine macroalgae with bioactivity against virulent and antibiotic resistant Vibrio , 2013 .

[17]  J. Imhoff,et al.  Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea , 2012 .

[18]  S. Sakthivelkumar,et al.  Screening of selected marine algae from the coastal Tamil Nadu, South India for antibacterial activity , 2012 .

[19]  P. Lawlor,et al.  Extraction and bioautographic-guided separation of antibacterial compounds from Ulva lactuca , 2012, Journal of Applied Phycology.

[20]  Li-Li Chen,et al.  Dietary administration of a Gracilaria tenuistipitata extract enhances the immune response and resistance against Vibrio alginolyticus and white spot syndrome virus in the white shrimp Litopenaeus vannamei. , 2011, Fish & shellfish immunology.

[21]  G. Pierre,et al.  Antibacterial activity of a sulfated galactan extracted from the marine alga Chaetomorpha aerea against Staphylococcus aureus , 2011 .

[22]  M. Won,et al.  Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. , 2011, Food chemistry.

[23]  M. Guedes,et al.  Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus , 2011 .

[24]  B. Withyachumnarnkul,et al.  Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon. , 2011, Fish & shellfish immunology.

[25]  Rafael dos Santos Rocha,et al.  Vibrio na água e sedimento de viveiros de quatro fazendas de carcinicultura no estado do Ceará, Brasil , 2010 .

[26]  Bouhlal Rhimou,et al.  The antibacterial potential of the seaweeds (Rhodophyceae) of the Strait of Gibraltar and the Mediterranean Coast of Morocco , 2010 .

[27]  F. Machado,et al.  Atividade biológica de metabólitos secundários de algas marinhas do gênero Laurencia , 2010 .

[28]  Z. Sekawi,et al.  Antibacterial Activity of Marine Source Extracts Against Multidrug Resistance Organisms , 2010 .

[29]  E. Ibáñez,et al.  Screening for bioactive compounds from algae. , 2010, Journal of pharmaceutical and biomedical analysis.

[30]  Ali A. El Gamal.  Biological importance of marine algae , 2009, Saudi Pharmaceutical Journal.

[31]  N. Abu-Ghannam,et al.  An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds , 2010 .

[32]  R. Seenivasan,et al.  The Antibacterial Activity of Some Marine Algae from South East Coast of India , 2010 .

[33]  S. Nabavi,et al.  Cytotoxic activity of some marine brown algae against cancer cell lines. , 2010, Biological research.

[34]  D. Stella,et al.  Antibacterial activity of marine macro algae against human pathogens , 2009 .

[35]  K. Mohamed,et al.  Screening of antibacterial activity in marine green and brown macroalgae from the coast of Morocco , 2009 .

[36]  T. Molinski,et al.  Drug development from marine natural products , 2009, Nature Reviews Drug Discovery.

[37]  Margareth Diniz,et al.  Constituintes químicos, avaliação das atividades citotóxica e antioxidante de Mimosa paraibana Barneby (Mimosaceae) , 2008 .

[38]  B. Baker,et al.  The Chemistry of Algal Secondary Metabolism , 2008 .

[39]  P. Jensen,et al.  Antimicrobial activities of extracts from tropical Atlantic marine plants against marine pathogens and saprophytes , 2006 .

[40]  A. L. Mathias,et al.  Chemical composition of Ulvaria oxysperma (Kützing) bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile) , 2004 .

[41]  Eliane C. Vidotti,et al.  Algas: da economia nos ambientes aquáticos à bioremediação e à química analítica , 2004 .

[42]  C. Vairappan.  Potent antibacterial activity of halogenated metabolites from Malaysian red algae, Laurencia majuscula (Rhodomelaceae, Ceramiales). , 2003, Biomolecular engineering.

[43]  M. Littler,et al.  Caribbean Reef Plants , 2000 .