Growth of Listeria monocytogenes in modified atmosphere packed cooked meat products: a predictive model

Abstract Predictive models for the influence of intrinsic and extrinsic parameters relevant for gas-packed, cooked meat products on the growth parameters (maximum specific growth rate and the lag phase ) of Listeria monocytogenes were developed. Two types of models were compared, i.e. extended Ratkowsky models and response surface models. Temperature, water activity, concentration of sodium lactate (SL) and dissolved CO 2 in the aqueous phase were considered as the relevant parameters influencing the growth of L. monocytogenes in gas-packed, cooked meat products and were included in the models. The concentration of CO 2 in the aqueous phase of the food was chosen as the determinative inhibitory factor of modified atmospheres. All four investigated independent variables did influence significantly the growth parameters of L. monocytogenes . Interactions between temperature and CO 2 and especially between CO 2 and Na–lactate were observed. The model was validated in real gas-packed, cooked meat products and was then combined with previously developed models for spoilage of gas-packed, cooked meat products to determine the ‘Risk Areas’ for these type of food products. Low water activity cooked meat products supported outgrowth of L. monocytogenes before spoilage had occurred, unless Na–lactate and CO 2 were applied as extra hurdles.

[1]  M C te Giffel,et al.  Validation of predictive models describing the growth of Listeria monocytogenes. , 1999, International journal of food microbiology.

[2]  H. Nissen,et al.  Addition of 2.5% lactate and 0.25% acetate controls growth of Listeria monocytogenes in vacuum-packed, sensory-acceptable servelat sausage and cooked ham stored at 4 degrees C. , 1997, International journal of food microbiology.

[3]  A H Geeraerd,et al.  Shelf life of modified atmosphere packed cooked meat products: addition of Na-lactate as a fourth shelf life determinative factor in a model and product validation. , 2000, International journal of food microbiology.

[4]  R. Beumer,et al.  Growth of Listeria monocytogenes on sliced cooked meat products. , 1996 .

[5]  J. Farber,et al.  Modelling the effects of various parameters on the growth of Listeria monocytogenes on liver pâté , 1995 .

[6]  J Debevere,et al.  Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. , 1999, International journal of food microbiology.

[7]  D W Schaffner,et al.  Evaluation of data transformations used with the square root and schoolfield models for predicting bacterial growth rate , 1992, Applied and environmental microbiology.

[8]  M. H. Zwietering,et al.  Evaluation of Data Transformations and Validation of a Model for the Effect of Temperature on Bacterial Growth , 1994, Applied and environmental microbiology.

[9]  J. Farber,et al.  Predictive modeling of the growth of Listeria monocytogenes in CO2 environments. , 1996, International journal of food microbiology.

[10]  B M Mackey,et al.  The effect of the growth environment on the lag phase of Listeria monocytogenes. , 1998, International journal of food microbiology.

[11]  M. Doyle,et al.  Fate of Listeria monocytogenes in processed meat products during refrigerated storage , 1989, Applied and environmental microbiology.

[12]  M. Rea,et al.  Development of a predictive model for growth of Listeria monocytogenes in a skim milk medium and validation studies in a range of dairy products. , 1996, The Journal of applied bacteriology.

[13]  John G Phillips,et al.  Response Surface Model for Predicting the Effects of Temperature pH, Sodium Chloride Content, Sodium Nitrite Concentration and Atmosphere on the Growth of Listeria monocytogenes. , 1990, Journal of food protection.

[14]  D. Portnoy,et al.  GROWTH OF LISTERIA MONOCYTOGENES , 1988 .

[15]  P. Vanderlinde,et al.  Aerobio Growth of Listeria monocytogenes on Beef Lean and Fatty Tissue: Equations Describing the Effects of Temperature and pH. , 1993, Journal of food protection.

[16]  Grahame W. Gould,et al.  Mechanisms of action of food preservation procedures. , 1989 .

[17]  A. Magnin,et al.  Growth of Aeromonas hydrophila in modified-atmosphere-packed cooked meat products , 2000 .

[18]  F. Rombouts,et al.  Modeling of the Bacterial Growth Curve , 1990, Applied and environmental microbiology.

[19]  J. Troller,et al.  Minimal water activity levels for growth and survival of Listeria monocytogenes and Listeria innocua. , 1992, International journal of food microbiology.

[20]  U. Schmidt,et al.  Behaviour of L. monocytogenes in vacuum-packed sliced frankfurter-type sausage. , 1990 .

[21]  N. Chen,et al.  Relationship Between Water Activity, Salts of Lactic Acid, and Growth of Listeria monocytogenes in a Meat Model System. , 1992, Journal of food protection.

[22]  Tom Ross,et al.  Predictive Microbiology : Theory and Application , 1993 .

[23]  T. A. Roberts,et al.  Predictive modelling of growth of Listeria monocytogenes. The effects on growth of NaCl, pH, storage temperature and NaNO2. , 1997, International journal of food microbiology.

[24]  A. P. Damoglou,et al.  Effects of irradiation dose and storage temperature on the growth of Listeria monocytogenes on poultry meat , 1993 .

[25]  P. Fernández,et al.  Predictive model of the effect of CO2, pH, temperature and NaCl on the growth of Listeria monocytogenes. , 1997, International journal of food microbiology.

[26]  F Devlieghere,et al.  Concentration of carbon dioxide in the water-phase as a parameter to model the effect of a modified atmosphere on microorganisms. , 1998, International journal of food microbiology.

[27]  C. E. Wolf,et al.  Improved method for quantification of the bacteriocin nisin. , 1996, The Journal of applied bacteriology.

[28]  H. Korkeala,et al.  Lactic acid and pH as indicators of spoilage for vacuum-packed cooked ring sausages. , 1990, International journal of food microbiology.

[29]  R. Ahvenainen,et al.  Interrelationship between microbial numbers and other parameters in the spoilage of vacuum-packed cooked ring sausages , 1987 .

[30]  F Devlieghere,et al.  Shelf life of modified atmosphere packed cooked meat products: a predictive model. , 1999, International journal of food microbiology.

[31]  V. Razavilar,et al.  Prediction of Listeria spp. growth as affected by various levels of chemicals, pH, temperature and storage time in a model broth. , 1998, Journal of food microbiology.

[32]  E. Borch,et al.  A model based on absorbance data on the growth rate of Listeria monocytogenes and including the effects of pH, NaCl, Na-lactate and Na-acetate. , 1999, International journal of food microbiology.

[33]  Donald W Schaffner,et al.  Modeling the Effect of Temperature on the Growth Rate and Lag Time of Listeria innocua and Listeria monocytogenes. , 1993, Journal of food protection.

[34]  L. Harris,et al.  Growth of Listeria monocytogenes and Yersinia enterocolitica on Cooked Modified-Atmosphere-Packaged Poultry in the Presence and Absence of a Naturally Occurring Microbiota , 1999, Applied and Environmental Microbiology.

[35]  J. V. Van Impe,et al.  Effect of dissolved carbon dioxide and temperature on the growth of Lactobacillus sake in modified atmospheres. , 1998, International journal of food microbiology.

[36]  T. Ross.  Indices for performance evaluation of predictive models in food microbiology. , 1996, The Journal of applied bacteriology.

[37]  M. Peck,et al.  Predictive models of the effect of temperature, pH and acetic and lactic acids on the growth of Listeria monocytogenes. , 1996, International journal of food microbiology.

[38]  A. Lebert,et al.  Development of twoListeria monocytogenesgrowth models in a meat broth and their application to beef meat , 1998 .

[39]  H. Korkeala,et al.  Differences in microbial growth in the surface layer and at the centre of vacuum-packed cooked ring sausages , 1987 .