A Simple LC-MS/MS Method for Determination of Magnolol in Rat Blood and its Application in a Pharmacokinetic Study

Abstract A simple LC-MS/MS method was developed for determination and pharmacokinetic study of magnolol in rat blood. Blood sample pretreatment involved a one-step extraction with methanol of 100 µL blood. The chromatographic separation was carried out on a Agilent Zobax SB C18 column with a mobile phase consisting of acetonitrile-0.2% formic acid (55:45, v/v) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via electro spray ionization source with positive mode. A high throughput was achieved with a run time of 4 min per sample. The standard curve for magnolol was linear (r > 0.999) over the concentration range of 2–1 000 ng/mL, with a lower limit of quantification of 2 ng/mL. The intra- and inter-day precision (relative standard deviation) values were not higher than 12% and the accuracy (relative error) was <5% at three quality control levels. This simple, fast and highly sensitive method was fully validated and successfully applied to a clinical pharmacokinetic study of magnolol in rats after oral administration.

[1]  Shiuan-Pey Lin,et al.  Pharmacokinetics, Bioavailability, and Tissue Distribution of Magnolol Following Single and Repeated Dosing of Magnolol to Rats , 2011, Planta medica.

[2]  Hyung‐Min Kim,et al.  Anticancer potential of magnolol for lung cancer treatment , 2011, Archives of pharmacal research.

[3]  S. Cho,et al.  Magnolol Inhibits LPS-induced NF-κB/Rel Activation by Blocking p38 Kinase in Murine Macrophages. , 2010, The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology.

[4]  Kwang-Kyun Park,et al.  Magnolol Suppresses Metastasis via Inhibition of Invasion, Migration, and Matrix Metalloproteinase-2/-9 Activities in PC-3 Human Prostate Carcinoma Cells , 2010, Bioscience, biotechnology, and biochemistry.

[5]  Yu-Tse Wu,et al.  Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection. , 2006, Biomedical chromatography : BMC.

[6]  A. Kotani,et al.  Determination of honokiol and magnolol by micro HPLC with electrochemical detection and its application to the distribution analysis in branches and leaves of Magnolia obovata. , 2005, Chemical & pharmaceutical bulletin.

[7]  T. Tsai,et al.  Effector mechanism of magnolol‐induced apoptosis in human lung squamous carcinoma CH27 cells , 2003, British journal of pharmacology.

[8]  H. Nagase,et al.  Magnolol has the ability to induce apoptosis in tumor cells. , 2002, Biological & pharmaceutical bulletin.

[9]  T. Tsai,et al.  Down-modulation of Bcl-XL, release of cytochrome c and sequential activation of caspases during honokiol-induced apoptosis in human squamous lung cancer CH27 cells. , 2002, Biochemical pharmacology.

[10]  Y. Ho,et al.  Molecular mechanisms of apoptosis induced by magnolol in colon and liver cancer cells , 2001, Molecular carcinogenesis.

[11]  Jongsung Lee,et al.  Anti-inflammatory effects of magnolol and honokiol are mediated through inhibition of the downstream pathway of MEKK-1 in NF-kappaB activation signaling. , 2005, Planta medica.

[12]  Chien-Huang Lin,et al.  Magnolol suppresses proliferation of cultured human colon and liver cancer cells by inhibiting DNA synthesis and activating apoptosis , 2002, Journal of cellular biochemistry.