Abstract An experimental research on magnetic concentrate of a hematite using the anionic reverse flotation technology in the flotation column was conducted. Through systematic studies in the lab, the optimal technological and operational conditions had been obtained as follows: the circulation pressure of 0.055 MPa, the feeding quantity of 160 g/min, the collector dosage of 800 g/t and the starch dosage of 900 g/t. The results show that the iron concentrate with grade of 67.37% can be achieved according to the flow sheet of one roughing and one scavenging under the optimal conditions, and also reflects the advantages of excellent selectivity and high recovery. The application of cyclonic static micro-bubble flotation column in hematite reverse flotation provides a new approach for hematite reverse flotation in China.