WBC subset analysis of WBC‐reduced platelet components

BACKGROUND: WBC‐reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content <5 × 106 per component. However, there may be differences in the efficacy of these techniques for removing certain WBC subsets.

[1]  J. Nelson,et al.  Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. , 1985, Science.

[2]  M. Loken,et al.  Routine quantitation of white cells as low as 0.001 per microL in platelet components , 1993, Transfusion.

[3]  H. Reiser,et al.  Replication of human cytomegalovirus in human peripheral blood T cells , 1986, Journal of virology.

[4]  Standards for blood banks and transfusion services. , 1977, QRB. Quality review bulletin.

[5]  S. Sowemimo-Coker,et al.  White cell subsets in apheresis and filtered platelet concentrates , 1998, Transfusion.

[6]  D. Epps,et al.  A flow cytometric method for counting very low levels of white cells in blood and blood components , 1993, Transfusion.

[7]  Leukocyte reduction and ultraviolet B irradiation of platelets to prevent alloimmunization and refractoriness to platelet transfusions. , 1997, The New England journal of medicine.

[8]  G. Schoch,et al.  A comparison of filtered leukocyte-reduced and cytomegalovirus (CMV) seronegative blood products for the prevention of transfusion-associated CMV infection after marrow transplant. , 1995, Blood.

[9]  E. Anaissie,et al.  Protection of human polymorphonuclear leukocyte function from the deleterious effects of isolation, irradiation, and storage by interferon‐γ and granulocyte‐colony‐stimulating factor , 1995, Transfusion.

[10]  H. Meryman Transfusion-induced alloimmunization and immunosuppression and the effects of leukocyte depletion. , 1989, Transfusion medicine reviews.

[11]  A. Aguzzi,et al.  A crucial role for B cells in neuroinvasive scrapie , 1997, Nature.

[12]  L. Dumont,et al.  Enhanced flow cytometric method for counting very low numbers of white cells in platelet products. , 1996, Cytometry.

[13]  A. Bitsch,et al.  Failure to detect human cytomegalovirus DNA in peripheral blood leukocytes of healthy blood donors by the polymerase chain reaction , 1992, Transfusion.

[14]  L. Goodnough,et al.  Reducing the Risk for Transfusion-transmitted Cytomegalovirus Infection , 1992, Annals of Internal Medicine.

[15]  A. Bitsch,et al.  Cytomegalovirus transcripts in peripheral blood leukocytes of actively infected transplant patients detected by reverse transcription-polymerase chain reaction. , 1993, The Journal of infectious diseases.