Inhibition of subtilisin BPN' with peptide chloromethyl ketones.

[1]  J. Kraut,et al.  Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. X-ray crystallographic comparison of lysine- and phenylalanine-polypeptide chloromethyl ketone-inhibited subtilisin. , 1976, The Journal of biological chemistry.

[2]  J. Coggins,et al.  Affinity labelling of proteinases with tryptic specificity by peptides with C-terminal lysine chloromethyl ketone. , 1974, The Biochemical journal.

[3]  J. Powers,et al.  Active-site specific inhibitors of elastase. , 1973, Biochemistry.

[4]  K. Morihara,et al.  Effect of secondary interaction on the enzymatic activity of subtilisin BPN′: Comparison with α‐chymotrypsin, trypsin, and elastase , 1973, FEBS letters.

[5]  K. Kurachi,et al.  Kinetics of the reaction of chymotrypsin A with peptide chloromethyl ketones in relation to its subsite specificity. , 1973, Biochemistry.

[6]  J. Kraut,et al.  Subtilisin; a stereochemical mechanism involving transition-state stabilization. , 1972, Biochemistry.

[7]  G. Cohen,et al.  Substrate binding site in bovine chymotrypsin A-gamma. A crystallographic study using peptide chloromethyl ketones as site-specific inhibitors. , 1971, Biochemistry.

[8]  A. Henderson.  Determination of initial enzyme reaction rates from spectrophotometric recordings: a rapid method. , 1971, Analytical biochemistry.

[9]  H. Tsuzuki,et al.  Comparison of various types of subtilisins: size and properties of the active site. , 1971, Biochemical and biophysical research communications.

[10]  H. Tsuzuki,et al.  Subtilisin BPN': kinetic study with oligopeptides. , 1970, Archives of biochemistry and biophysics.

[11]  J. Powers,et al.  Design and synthesis of inhibitors for crystallographic studies on the active site of chymotrypsin. , 1970, Journal of the American Chemical Society.

[12]  E. Shaw,et al.  The essentiality of histidine in the catalytic action of subtilisin. Covalent modification by a specific reagent. , 1968, The Journal of biological chemistry.

[13]  A. Berger,et al.  On the size of the active site in proteases. I. Papain. , 1967, Biochemical and biophysical research communications.

[14]  C. Kasper,et al.  The complete amino acid sequence of two types of subtilisin, BPN' and Carlsberg. , 1966, The Journal of biological chemistry.

[15]  E. Shaw,et al.  Direct evidence for the presence of histidine in the active center of chymotrypsin. , 1963, Biochemistry.

[16]  R. Kitz,et al.  Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase. , 1962, The Journal of biological chemistry.