Incorporation of Specific Fatty Acid Precursors During Spore Germination and Outgrowth in Bacillus thuringiensis

The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [14C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-numbered iso-isomers, odd-numbered normal-chain acids, and odd-numbered isohomologs, respectively. This preferential incorporation by B. thuringiensis allows the terminal carbons of specific normal and branched-chain fatty acids, contained within the cytoplasmic membrane, to be labeled with 14C and, potentially, 13C.

[1]  L. Ingram,et al.  Propionate-induced synthesis of odd-chain-length fatty acids by Escherichia coli , 1977, Journal of bacteriology.

[2]  T. Kaneda.  Fatty acids of the genus Bacillus: an example of branched-chain preference. , 1977, Bacteriological reviews.

[3]  K. Nickerson,et al.  Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis , 1975, Journal of bacteriology.

[4]  K. Nickerson,et al.  Lipid metabolism during bacterial growth, sporulation, and germination: kinetics of fatty acid and macromolecular synthesis during spore germination and outgrowth of Bacillus thuringiensis , 1975, Journal of bacteriology.

[5]  H. Daron.  Nutritional Alteration of the Fatty Acid Composition of a Thermophilic Bacillus Species , 1973, Journal of bacteriology.

[6]  K. Willecke,et al.  Fatty acid-requiring mutant of bacillus subtilis defective in branched chain alpha-keto acid dehydrogenase. , 1971, The Journal of biological chemistry.

[7]  K. Bloch,et al.  Comparative aspects of fatty acid synthesis in Bacillus subtilis and Escherichia coli. , 1970, European journal of biochemistry.

[8]  M. Daniels.  Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coli. , 1969, The Biochemical journal.

[9]  A Kornberg,et al.  Biochemical Studies of Bacterial Sporulation and Germination XV. Fatty Acids in Growth, Sporulation, and Germination of Bacillus megaterium , 1969, Journal of bacteriology.

[10]  T. Kaneda.  Biosynthesis of branched chain fatty acids. II. Microbial synthesis of branched long chain fatty acids from certain short chain fatty acid substrates. , 1963, The Journal of biological chemistry.

[11]  G. Wegner,et al.  INCORPORATION OF ISOBUTYRATE AND VALERATE INTO CELLULAR PLASMALOGEN BY BACTEROIDES SUCCINOGENES , 1963, Journal of bacteriology.

[12]  K. Bloch.  Fatty acid synthases from Mycobacterium phlei. , 1975, Methods in enzymology.

[13]  H. J. Dutton,et al.  Improved resolution in high-sensitivity dual-label gas radiochromatography , 1969 .