Chapter 1 Measurement of Cell Volumes by Electric Sensing Zone Instruments

Publisher Summary This chapter discusses the accuracy of measurement of volume distributions by electric-sensing zone instruments currently in use. The various electric sensing zone instruments are the Coulter Counter Model B (Coulter, 1953, Coulter Electronics Inc., Hialeah, Florida), (2) the instrument described by Harvey and Marr, (3) the Celloscope 101 (Particle Data Inc., Elmhurst, Illinois), (4) the Nuclear Chicago particle measurement system (Nuclear Chicago Corp., Des Plaines, Illinois). The measurement of particle concentrations and volumes by electric-sensing zone instruments has found wide application in recent years. Such instruments are capable of rapid and accurate measurement of particle concentrations, and of more or less accurate determinations of volume distributions of particle populations. The accuracy and fidelity of measurement of volume is by no means as certain nor is it easily determined. The only attempt at rigorous evaluation of the fidelity of measurement of volume distribution is that of Harvey and Marr. The transducer is a cylindrical aperture separating two-electrode chambers filled with electrolyte.