Airborne Transmission of a Serotype 4 Fowl Adenovirus in Chickens

Serotype 4 fowl adenovirus (FAdV-4) is the main pathogen for hydropericardium syndrome (HPS) in chickens. It has caused major economic losses in the global poultry industry. Currently, FAdV-4′s transmission routes in chickens remain unclear. Here we investigate the airborne transmission routes of FAdV-4 in chickens. A total of 45 ten-day-old chickens were equally divided into three groups (infected group/isolator A, airborne group/isolator B, and control group/isolator C). Of note, isolators A and B were connected by a leak-free pipe. The results showed that the virus could form a viral aerosol, detected in isolators two days post infection (dpi). The viral aerosol reached a peak at 4 dpi in the infected group. Healthy chickens in the airborne group were infected by the virus at 8 dpi. The chickens of the airborne group demonstrated subclinical symptoms capable of shedding the virus for some time. This finding suggests that FAdV-4 can be efficiently transmitted among chickens by aerosol transmission. These findings have significant implications for developing strategies to control this infectious disease epidemic.

[1]  Y. Diao,et al.  Pathogenic, Phylogenetic, and Serological Analysis of Group I Fowl Adenovirus Serotype 4 SDSX Isolated From Shandong, China , 2018, Front. Microbiol..

[2]  Hongning Wang,et al.  Complete genome sequence and pathogenicity of fowl adenovirus serotype 4 involved in hydropericardium syndrome in Southwest China. , 2018, Microbial pathogenesis.

[3]  Sidang Liu,et al.  Epidemiological investigation of outbreaks of fowl adenovirus infections in commercial chickens in China , 2018, Transboundary and emerging diseases.

[4]  Q. Luo,et al.  Fowl adenovirus serotype 4: Epidemiology, pathogenesis, diagnostic detection, and vaccine strategies , 2017, Poultry science.

[5]  Xiaomei Wang,et al.  The first whole genome sequence and pathogenicity characterization of a fowl adenovirus 4 isolated from ducks associated with inclusion body hepatitis and hydropericardium syndrome , 2017, Avian pathology : journal of the W.V.P.A.

[6]  Jun Luo,et al.  Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China , 2016, Virology Journal.

[7]  Jun Luo,et al.  Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China , 2016, Virology Journal.

[8]  Shengwang Liu,et al.  Fowl adenovirus species C serotype 4 is attributed to the emergence of hepatitis-hydropericardium syndrome in chickens in China. , 2016, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[9]  Yang Li,et al.  Isolation, identification, and hexon gene characterization of fowl adenoviruses from a contaminated live Newcastle disease virus vaccine , 2016, Poultry science.

[10]  Wei Sun,et al.  Hydropericardium syndrome outbreak caused by fowl adenovirus serotype 4 in China in 2015. , 2016, The Journal of general virology.

[11]  Dong-sheng Gao,et al.  Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China , 2016, Emerging Microbes & Infections.

[12]  J. Niczyporuk.  Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland , 2015, Archives of Virology.

[13]  Zejun Li,et al.  Airborne Transmission of a Novel Tembusu Virus in Ducks , 2015, Journal of Clinical Microbiology.

[14]  Fangshan Wang,et al.  Generation, transmission and infectiosity of chicken MDV aerosols under experimental conditions. , 2014, Veterinary microbiology.

[15]  M. Hess,et al.  Recombinant FAdV-4 fiber-2 protein protects chickens against hepatitis-hydropericardium syndrome (HHS). , 2014, Vaccine.

[16]  S. Sharif,et al.  Pathogenicity and Cytokine Gene Expression Pattern of a Serotype 4 Fowl Adenovirus Isolate , 2013, PloS one.

[17]  S. Luo,et al.  Detection of antibodies specific to the non-structural proteins of fowl adenoviruses in infected chickens but not in vaccinated chickens , 2013, Avian pathology : journal of the W.V.P.A.

[18]  Yuwei Gao,et al.  Experimental transmission in guinea pigs of H9N2 avian influenza viruses from indoor air of chicken houses. , 2012, Virus research.

[19]  E. Nagy,et al.  Coding potential and transcript analysis of fowl adenovirus 4: insight into upstream ORFs as common sequence features in adenoviral transcripts. , 2011, The Journal of general virology.

[20]  Cuiping Song,et al.  Occurrence and transmission of Newcastle disease virus aerosol originating from infected chickens under experimental conditions. , 2009, Veterinary microbiology.

[21]  J. Valarcher,et al.  Airborne transmission of foot-and-mouth disease in pigs: evaluation and optimisation of instrumentation and techniques. , 2009, Veterinary journal.

[22]  John Steel,et al.  High Temperature (30°C) Blocks Aerosol but Not Contact Transmission of Influenza Virus , 2008, Journal of Virology.

[23]  D. Ojkic,et al.  Study of vertical transmission of fowl adenoviruses. , 2006, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[24]  C. Tseng,et al.  Collection efficiencies of aerosol samplers for virus-containing aerosols , 2005, Journal of Aerosol Science.

[25]  Alan M. Jones,et al.  The effects of meteorological factors on atmospheric bioaerosol concentrations--a review. , 2004, The Science of the total environment.

[26]  C. S. Kristensen,et al.  Experimental airborne transmission of PRRS virus. , 2004, Veterinary microbiology.

[27]  J. Kataria,et al.  The Hydropericardium Syndrome in Poultry – A Current Scenario , 2004, Veterinary Research Communications.

[28]  K. Lager,et al.  Experimental airborne transmission of porcine reproductive and respiratory syndrome virus and Bordetella bronchiseptica. , 2002, Veterinary microbiology.

[29]  M. D. de Jong,et al.  Airborne transmission of bovine herpesvirus 1 infections in calves under field conditions. , 2000, Veterinary microbiology.

[30]  K. Naeem,et al.  Hydropericardium syndrome outbreak in a pigeon flock , 1995, Veterinary Record.

[31]  D. Alexander.  The epidemiology and control of avian influenza and Newcastle disease. , 1995, Journal of comparative pathology.

[32]  M. Hugh-jones,et al.  The evidence for the airborne spread of Newcastle disease , 1973, Journal of Hygiene.

[33]  R. Cumming.  Studies on Australian infectious bronchitis virus. IV. Apparent farm-to-farm airborne transmission of infectious bronchitis virus. , 1970, Avian diseases.

[34]  J. Niczyporuk.  Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland , 2015, Archives of Virology.

[35]  R. Kumar,et al.  Isolation and Identification of a Fowl Adenovirus from Wild Black Kites (Milvus migrans) , 2010, Journal of wildlife diseases.

[36]  M. Hess,et al.  Fowl Adenovirus Serotype 4 Associated with Outbreaks of Infectious Hydropericardium in Haryana, India , 2002, Avian diseases.

[37]  R. T. Dubose.  Quail Bronchitis1 , 1967 .