A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity.

[1]  D. Hazuda,et al.  Inhibition of HIV-1 Ribonuclease H by a Novel Diketo Acid, 4-[5-(Benzoylamino)thien-2-yl]-2,4-dioxobutanoic Acid* , 2003, The Journal of Biological Chemistry.

[2]  A M Gewirtz,et al.  Chimeric RNA-DNA molecular beacon assay for ribonuclease H activity. , 2002, Molecular and cellular probes.

[3]  L. Menéndez-Arias Targeting HIV: antiretroviral therapy and development of drug resistance. , 2002, Trends in pharmacological sciences.

[4]  M. Wainberg,et al.  Nonradioactive detection of retroviral-associated RNase H activity in a microplate-based, high-throughput format. , 2002, BioTechniques.

[5]  N. Sluis-Cremer,et al.  Destabilization of the HIV-1 reverse transcriptase dimer upon interaction with N-acyl hydrazone inhibitors. , 2002, Molecular pharmacology.

[6]  M. Hattori,et al.  Inhibitory effects of quinones on RNase H activity associated with HIV‐1 reverse transcriptase , 2002, Phytotherapy research : PTR.

[7]  S. L. Le Grice,et al.  Evaluation of retroviral ribonuclease H activity. , 2001, Methods in molecular biology.

[8]  D. Hupe,et al.  Inhibitors of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. , 1999, Biochemistry.

[9]  Thomas D. Y. Chung,et al.  A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays , 1999, Journal of biomolecular screening.

[10]  W. Ma,et al.  Ribonuclease H renaturation gel assay using a fluorescent-labeled substrate. , 1997, BioTechniques.

[11]  M. Parniak,et al.  Inhibition of the ribonuclease H and DNA polymerase activities of HIV-1 reverse transcriptase by N-(4-tert-butylbenzoyl)-2-hydroxy-1-naphthaldehyde hydrazone. , 1997, Biochemistry.

[12]  P. Štrop,et al.  Development of a nonradioactive ribonuclease H assay. , 1996, Analytical biochemistry.

[13]  M. Wainberg,et al.  Single-step purification of recombinant wild-type and mutant HIV-1 reverse transcriptase. , 1996, Protein expression and purification.

[14]  S. Goff,et al.  Assays for retroviral reverse transcriptase. , 1995, Methods in enzymology.

[15]  J. Toulmé,et al.  Sensitive detection of low levels of ribonuclease H activity by an improved renaturation gel assay. , 1993, Biochemical and biophysical research communications.

[16]  A. Hizi,et al.  The interaction of illimaquinone, a selective inhibitor of the RNase H activity, with the reverse transcriptases of human immunodeficiency and murine leukemia retroviruses. , 1993, The Journal of biological chemistry.

[17]  B. Larder,et al.  Mutations within the RNase H domain of human immunodeficiency virus type 1 reverse transcriptase abolish virus infectivity. , 1991, The Journal of general virology.

[18]  S. L. Le Grice,et al.  HIV‐1 RT‐associated ribonuclease H displays both endonuclease and 3′‐‐‐‐5′ exonuclease activity. , 1990, The EMBO journal.

[19]  Y. Cheng,et al.  Human immunodeficiency virus reverse transcriptase-associated RNase H activity. , 1989, The Journal of biological chemistry.

[20]  P. Ponka,et al.  Synthesis of new acylhydrazones as iron-chelating compounds , 1988 .