Gradient Elution Centrifugal Partition Chromatography: Comparison with HPLC Gradients and use of Ternary Diagrams to Build Gradients

Abstract Just as with HPLC, gradient elution provides to CPC an easy way to fractionate solutes of widely differing polarities and partition coefficients, and to reduce run times. The easiest way to design a gradient for CPC is to refer to ternary diagrams corresponding to the ternary liquid mixtures generally encountered in centrifugal partition chromatography.