Characterization of a Novel YnSKn Class of Dehydrin-Like cDNAs from Cold Acclimated Red-Osier Dogwood (Cornus sericea L.) Xylem

Summary Red-osier dogwood (Cornus sericea L.) is among the most freeze-tolerant plant species. Four cDNAs were isolated from cold acclimated red-osier dogwood xylem and found to encode novel dehydrin-like proteins. The four deduced amino acid sequences were extremely hydrophilic and contained sequence motifs characteristic of the dehydrin family. ROD (red-osier dehydrin) 44, 48 and 60 sequences each contained the Y, S, and K motifs characteristic of dehydrins. Whereas ROD 25 contained the Y and S motifs but lacked the lysine-rich repeat (K segment). A unique aspect of these sequences was that ROD 25, 44, 48 and 60 included 14, 33, 33 and 35 copies, respectively, of a sequence similar to DEYGNP. This is quite different from other dehydrin-like proteins, which typically contained only one to three Y motifs. The function of these highly repeated sequences is not known. Alignment of the deduced amino acid sequences of ROD 44 and ROD 48 revealed 69% identity. Alignment of overlapping regions of ROD 25 and ROD 60 revealed 89% identity. Transcript expression of Rod genes changed seasonally in red-osier dogwood and was correlated to periods of cold acclimation.

[1]  E. Ashworth,et al.  Seasonal regulation of a 24-kDa protein from red-osier dogwood (Cornus sericea) xylem. , 2002, Tree physiology.

[2]  R. Arora,et al.  A 25-kDa dehydrin associated with genotype- and age-dependent leaf freezing-tolerance in Rhododendron: a genetic marker for cold hardiness? , 1999, Theoretical and Applied Genetics.

[3]  P. Rinne,et al.  Dehydrins in cold-acclimated apices of birch (Betula pubescens Ehrh.): production, localization and potential role in rescuing enzyme function during dehydration , 1999, Planta.

[4]  R. Pearce.  Molecular analysis of acclimation to cold , 1999, Plant Growth Regulation.

[5]  Michael F. Thomashow,et al.  PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms. , 1999, Annual review of plant physiology and plant molecular biology.

[6]  D. Takezawa,et al.  Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation. , 1999, Plant physiology.

[7]  T. Close,et al.  Purification, immunolocalization, cryoprotective, and antifreeze activity of PCA60: A dehydrin from peach (Prunus persica) , 1999 .

[8]  M. Thomashow.  Role of cold-responsive genes in plant freezing tolerance. , 1998, Plant physiology.

[9]  P. Rinne,et al.  Onset of freezing tolerance in birch (Betula pubescens Ehrh.) involves LEA proteins and osmoregulation and is impaired in an ABA‐deficient genotype , 1998 .

[10]  C. Guy,et al.  Association of Proteins with the Stress 70 Molecular Chaperones at Low Temperature: Evidence for the Existence of Cold Labile Proteins in Spinach☆ , 1998 .

[11]  T. Close,et al.  Temporal accumulation and ultrastructural localization of dehydrins in Zea mays , 1997 .

[12]  T. Close,et al.  Dehydrins: genes, proteins, and associations with phenotypic traits , 1997 .

[13]  T. Close.  Dehydrins: A commonalty in the response of plants to dehydration and low temperature , 1997 .

[14]  P. Rinne,et al.  Photoperiodic induction of dormancy and freezing tolerance in Betula pubescens. Involvement of ABA and dehydrins , 1997 .

[15]  M. Wisniewski,et al.  Accumulation of a 60-kD Dehydrin Protein in Peach Xylem Tissues and Its Relationship to Cold Acclimation , 1996 .

[16]  M. Wisniewski,et al.  Cold Acclimation and Alterations in Dehydrin-like and Bark Storage Proteins in the Leaves of Sibling Deciduous and Evergreen Peach , 1996 .

[17]  T. Close.  Dehydrins: Emergence of a biochemical role of a family of plant dehydration proteins , 1996 .

[18]  R. Bressan,et al.  Programmed accumulation of LEA-like proteins during desiccation and cold acclimation of overwintering grape buds , 1996 .

[19]  T. Close,et al.  Seasonal patterns of dehydrins and 70-kDa heat-shock proteins in bark tissues of eight species of woody plants , 1996 .

[20]  F. Allard,et al.  Immunolocalization of freezing-tolerance-associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues. , 1995, The Plant journal : for cell and molecular biology.

[21]  S. Hill,et al.  Cold-Induced Accumulation of hsp90 Transcripts in Brassica napus , 1995, Plant physiology.

[22]  S. Torres-Schumann,et al.  Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants , 1994, Plant Molecular Biology.

[23]  T. Close,et al.  Nuclear and cytoplasmic localization of maize embryo and aleurone dehydrin , 1994, Protoplasma.

[24]  M. Wisniewski,et al.  Cold Acclimation in Genetically Related (Sibling) Deciduous and Evergreen Peach (Prunus persica [L.] Batsch) (II. A 60-Kilodalton Bark Protein in Cold-Acclimated Tissues of Peach Is Heat Stable and Related to the Dehydrin Family of Proteins) , 1994, Plant physiology.

[25]  C. Guy,et al.  Structural Organization of the Spinach Endoplasmic Reticulum-Luminal 70-Kilodalton Heat-Shock Cognate Gene and Expression of 70-Kilodalton Heat-Shock Genes during Cold Acclimation , 1994, Plant physiology.

[26]  L. Rowland,et al.  Identification of Dehydrin-Like Proteins Responsive to Chilling in Floral Buds of Blueberry (Vaccinium, section Cyanococcus) , 1994, Plant physiology.

[27]  M. Mar Albà,et al.  The maize abscisic acid-responsive protein Rab17 is located in the nucleus and interacts with nuclear localization signals. , 1994, The Plant cell.

[28]  F. Hartl,et al.  Identification of nucleotide-binding regions in the chaperonin proteins GroEL and GroES , 1993, Nature.

[29]  T. Close,et al.  A view of plant dehydrins using antibodies specific to the carboxy terminal peptide , 1993, Plant Molecular Biology.

[30]  Timothy J. Close,et al.  Plant responses to cellular dehydration during environmental stress , 1993 .

[31]  H. Ougham,et al.  Gene expression under temperature stress. , 1993, The New phytologist.

[32]  J. Cairney,et al.  A simple and efficient method for isolating RNA from pine trees , 1993, Plant Molecular Biology Reporter.

[33]  L. Dure.  A repeating 11-mer amino acid motif and plant desiccation. , 1993, The Plant journal : for cell and molecular biology.

[34]  M. Werner-Washburne,et al.  The translation machinery and 70 kd heat shock protein cooperate in protein synthesis , 1992, Cell.

[35]  W. Welch,et al.  Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly. , 1990, Science.

[36]  L. Dure,et al.  Sequence and characterization of 6 Lea proteins and their genes from cotton , 1988, Plant Molecular Biology.

[37]  G. Galau,et al.  Developmental biochemistry of cottonseed embryogenesis and germination , 1983, Plant Molecular Biology.

[38]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[39]  G. Galau,et al.  Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. , 1981, Biochemistry.

[40]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[41]  D. Kemp,et al.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[42]  M. Burke,et al.  Freezing and Injury in Plants , 1976 .

[43]  P. Kramer,et al.  Responses of Plants to Environmental Stresses , 1973 .

[44]  P. Leder,et al.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[45]  C. J. Weiser,et al.  Cold Resistance and Injury in Woody Plants , 1970, Science.

[46]  A. Wood.  Molecular, biochemical and physiological responses of sorghum to pre-flowering drought stress: The role of dehydrins, glycinebetaine and osmotic adjustment as protective mechanisms , 1994 .

[47]  M. Thomashow.  Molecular Genetics of Cold Acclimation in Higher Plants , 1990 .

[48]  Charles L. Guy,et al.  Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism , 1990 .

[49]  J. Levitt,et al.  Responses of Plants to Environmental Stress, 2nd Edition, Volume 1: Chilling, Freezing, and High Temperature Stresses. , 1980 .